Abstract
ε-Poly-l-lysine (ε-PL), one of the only two homo-poly amino acids known in nature, is used as a preservative. In this study, strategies of feeding precursor l-lysine into 5 L laboratory scale fermenters, including optimization of l-lysine concentration and time, was investigated to optimize the production of ε-PL by Streptomyces sp. M-Z18. The optimized strategy was then used in ε-PL fed-batch fermentation in which glucose and glycerol served as mixed carbon sources. In this way, a novel ε-PL production strategy involving precursor l-lysine coupled with glucose–glycerol co-fermentation was developed. Under optimal conditions, ε-PL production reached 37.6 g/l, which was 6.2 % greater than in a previous study in which glucose and glycerol co-fermentation was performed without added l-lysine (35.14 g/l). To the best of our knowledge, this is the first report of the enhancement of ε-PL production through l-lysine feeding to evaluate the use of fermenters. Meanwhile, the role of l-lysine in the promotion of ε-PL production, participating ε-PL synthesis as a whole, was first determined using the l-[U–13C] lysine labeling method. It has been suggested that the bottleneck of ε-PL synthesis in Streptomyces sp. M-Z18 is in the biosynthesis of precursor l-lysine. The information obtained in the present work may facilitate strain improvement and efficient large-scale ε-PL production.
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Acknowledgments
This work was financially supported by the Jiangsu Key Project of Scientific and Technical Supporting Program (BE2012616), the Fundamental Research Funds for the Central Universities (JUSRP11217), the Wuxi Science and Technology Support Program (CYE21N1107), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the 111 Project (111-2-06).
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Chen, XS., Ren, XD., Zeng, X. et al. Enhancement of ε-poly-l-lysine production coupled with precursor l-lysine feeding in glucose–glycerol co-fermentation by Streptomyces sp. M-Z18. Bioprocess Biosyst Eng 36, 1843–1849 (2013). https://doi.org/10.1007/s00449-013-0958-7
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DOI: https://doi.org/10.1007/s00449-013-0958-7