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Acidic pH shock induced overproduction of ε-poly-l-lysine in fed-batch fermentation by Streptomyces sp. M-Z18 from agro-industrial by-products

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Abstract

ε-Poly-l-lysine (ε-PL) is produced by Streptomyces as a secondary metabolite with wide industrial applications, but its production still needs to be further enhanced. Environmental stress is an important approach for the promotion of secondary metabolites production by Streptomyces. In this study, the effect of acidic pH shock on enhancing ε-PL production by Streptomyces sp. M-Z18 was investigated in a 5-L fermenter. Based on the evaluation of acidic pH shock on mycelia metabolic activity and shock parameters optimization, an integrated pH-shock strategy was developed as follows: pre-acid-shock adaption at pH 5.0 to alleviate the damage caused by the followed pH shock, and then acidic pH shock at 3.0 for 12 h (including pH decline from 4.0 to 3.0) to positively regulate mycelia metabolic activity, finally restoring pH to 4.0 to provide optimal condition for ε-PL production. After 192 h of fed-batch fermentation, the maximum ε-PL production and productivity reached 54.70 g/L and 6.84 g/L/day, respectively, which were 52.50 % higher than those of control without pH shock. These results demonstrated that acidic pH shock is an efficient approach for improving ε-PL production. The information obtained should be useful for ε-PL production by other Streptomyces.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (31301556), the Jiangsu Key Project of Scientific and Technical Supporting Program (BE2012616), the Cooperation Project of Jiangsu Province among Industries, Universities and Institutes (BY2013015-11), the Jiangsu Province “Collaborative Innovation Center for Advanced Industrial Fermentation” Industry Development Program, the Priority Academic Program Development of Jiangsu Higher Education Institutions, the 111 Project (111-2-06) and the National Natural Science Foundation of China (21376106).

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China

    Xi-Dong Ren, Xu-Sheng Chen, Xin Zeng, Liang Wang, Lei Tang & Zhong-Gui Mao

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  1. Xi-Dong Ren
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  2. Xu-Sheng Chen
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  3. Xin Zeng
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  4. Liang Wang
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  5. Lei Tang
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Correspondence to Xu-Sheng Chen or Zhong-Gui Mao.

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Ren, XD., Chen, XS., Zeng, X. et al. Acidic pH shock induced overproduction of ε-poly-l-lysine in fed-batch fermentation by Streptomyces sp. M-Z18 from agro-industrial by-products. Bioprocess Biosyst Eng 38, 1113–1125 (2015). https://doi.org/10.1007/s00449-015-1354-2

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