Abstract
Melting of sea-ice samples is an inevitable step in obtaining reliable and representative measurements for biogeochemical parameters such as inorganic nutrients and particulate matter. The impact of the sea-ice melting procedure has been previously evaluated for biological parameters such as chlorophyll a and cell abundance. For nutrient and biomass concentrations in sea ice, it is generally considered to be best practice to melt samples fast; however, no systematic evaluation exists in literature. The impact of melting temperature and buffer addition to avoid osmotic shock was tested on ice samples in Saroma-ko Lagoon on the northeastern coast of Hokkaido, Japan. The focus was on inorganic nutrient concentrations (NO3−, NO2−, PO4−, NH4+, Si(OH)4) and particulate organic carbon and nitrogen concentrations and their isotope ratios. Coherent small changes have been observed for the parameter related to nitrogen, suggesting marginal cell lysis of a specific part of the microbial community. When differences are statistically significant, they are close to the uncertainty of the measurements and small in regard to the expected natural variation in sea ice. Our study suggest a minimal effect between melting treatments on biomass (POC, PN, and Chl a) and nutrient measurements in diatom-dominated sea ice and should be repeated where the sympagic community is dominated by flagellates.
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References
Altabet MA (1988) Variations in nitrogen isotopic composition between sinking and suspended particles: implications for nitrogen cycling and particle transformation in the open ocean. Deep Sea Res Part A 35:535–554. https://doi.org/10.1016/0198-0149(88)90130-6
Baer SE, Connelly TL, Bronk DA (2015) Nitrogen uptake dynamics in landfast sea ice of the Chukchi Sea. Polar Biol 38:781–797. https://doi.org/10.1007/s00300-014-1639-y
Cota GF, Anning JL, Harris LR et al (1990) Impact of ice algae on inorganic nutrients in seawater and sea ice in Barrow Strait, NWT, Canada, during spring. Can J Fish Aquat Sci 47:1402–1415
Dore JE, Houlihan T, Hebel DV et al (1996) Freezing as a method of sample preservation for the analysis of dissolved inorganic nutrients in seawater. Mar Chem 53:173–185. https://doi.org/10.1016/0304-4203(96)00004-7
Dortch Q, Clayton JR, Thoresen SS, Ahmed SI (1984) Species differences in accumulation of nitrogen pools in phytoplankton. Mar Biol 81:237–250. https://doi.org/10.1007/BF00393218
Fawcett SE, Lomas M, Casey JR et al (2011) Assimilation of upwelled nitrate by small eukaryotes in the Sargasso Sea. Nat Geosci 4:717–722. https://doi.org/10.1038/Ngeo1265
Fripiat F, Sigman DM, Fawcett SE et al (2014) New insights into sea ice nitrogen biogeochemical dynamics from the nitrogen isotopes. Global Biogeochem Cycles 28:115–130. https://doi.org/10.1002/2013GB004729
Fripiat F, Meiners KM, Vancoppenolle M et al (2017) Macro-nutrient concentrations in Antarctic pack ice : overall patterns and overlooked processes. Elem Sci Anthr 5:13. https://doi.org/10.1525/elementa.217
Fripiat F, Sigman DM, Massé G, Tison J-L (2015) High turnover rates indicated by changes in the fixed N forms and their stable isotopes in Antarctic landfast sea ice. J Geophys Res 120:3079–3097. https://doi.org/10.1002/2014JC010583
Garrison DL, Buck KR (1986) Organism losses during ice melting: a serious bias in sea ice community studies. Polar Biol 6:237–239
Guerrero MA, Jones RD (1996) Photoinhibition of marine nitrifying bacteria. I. Wavelength-dependent response. Mar Ecol Prog Ser 141:183–192. https://doi.org/10.3354/meps141183
Garrison DL, Sullivan CW, Ackley SF, Sullivan W (1986) Microbial Communitie in Antarctica These communities may provide an important. Bioscience 36:243–250
Kovacs A (1996) Part I. Bulk salinity versus ice floe thickness. CRREL Rep 96:1–16
Kottmeier ST, Sullivan CW (1987) Late winter primary production and bacterial production in sea ice and seawater west of the Antarctic Peninsula. Mar Ecol Prog Ser 36:287–298. https://doi.org/10.3354/Meps036287
Kaartokallio H, Kuosa H, Thomas DN et al (2007) Biomass, composition and activity of organism assemblages along a salinity gradient in sea ice subjected to river discharge in the Baltic Sea. Polar Biol 30:183–197. https://doi.org/10.1007/s00300-006-0172-z
Kristiansen S, Syvertsen EE, Farbrot T (1992) Nitrogen uptake in the Weddell Sea during late winter and spring. Polar Biol 12:245–251. https://doi.org/10.1007/BF00238266
Kamp A, Høgslund S, Risgaard-Petersen N, Stief P (2015) Nitrate storage and dissimilatory nitrate reduction by eukaryotic microbes. Front Microbiol 6:1–15. https://doi.org/10.3389/fmicb.2015.01492
Langway CC (1958) Ice fabrics and the universal stage. CRREL Rep 62:1–16
Lomas MW, Glibert PM (2000) Comparisons of nitrate uptake, storage, and reduction in marine diatoms and flagellates. J Phycol 913:903–913
Mikkelsen DM, Witkowski A (2010) Melting sea ice for taxonomic analysis: a comparison of four melting procedures. Polar Res 29:451–454. https://doi.org/10.1111/j.1751-8369.2010.00162.x
McMinn A, Gradinger R, Nomura D (2009) Biogeochemical properties of sea ice. In: Eicken H, Gradinger R, Shirasawa K, et al. (eds) Field techniques for sea ice research. University of Alaska Press, Fairbanks, pp 259–282
Merbt SN, Stahl DA, Casamayor EO et al (2012) Differential photoinhibition of bacterial and archaeal ammonia oxidation. FEMS Microbiol Lett 327:41–46. https://doi.org/10.1111/j.1574-6968.2011.02457.x
Munro DR, Dunbar RB, Mucciarone DA et al (2010) Stable isotope composition of dissolved inorganic carbon and particulate organic carbon in sea ice from the Ross Sea, Antarctica. J Geophys Res Ocean 115:1–14. https://doi.org/10.1029/2009JC005661
McMinn A, Hattori H, Hirawake T, Iwamoto A (2008) Preliminary investigation of Okhotsk Sea ice algae; taxonomic composition and photosynthetic activity. Polar Biol 31:1011–1015. https://doi.org/10.1007/s00300-008-0433-0
Meiners KM, Brinkmeyer R, Granskog MA, Lindfors A (2004) Abundance, size distribution and bacterial colonization of exopolymer particles in Antarctic sea ice (Bellingshausen Sea). Aquat Microb Ecol 35:283–296. https://doi.org/10.3354/ame035283
Nomura D, Takatsuka T, Ishikawa M et al (2009) Transport of chemical components in sea ice and under-ice water during melting in the seasonally ice-covered Saroma-ko Lagoon, Hokkaido, Japan. Estuar Coast Shelf Sci 81:201–209. https://doi.org/10.1016/j.ecss.2008.10.012
Nomura D, McMinn A, Hattori H et al (2011) Incorporation of nitrogen compounds into sea ice from atmospheric deposition. Mar Chem 127:90–99
Parsons TR, Maita Y, Lalli CM (1985) A manual of chemical and biological methods for seawater analysis. Pergamon Press, Oxford
Rintala J-MM, Piiparinen J, Blomster J et al (2014) Fast direct melting of brackish sea-ice samples results in biologically more accurate results than slow buffered melting. Polar Biol 37:1811–1822. https://doi.org/10.1007/s00300-014-1563-1
Robineau B, Legendre L, Kishino M, Kudoh S (1997) Horizontal heterogeneity of microalgal biomass in the first-year sea ice of Saroma-ko Lagoon (Hokkaido, Japan). J Mar Syst 11:81–91. https://doi.org/10.1016/S0924-7963(96)00030-9
Shirasawa K, Leppäranta M (2003) Hydrometeorological and sea ice conditions at Saroma-ko lagoon, Hokkaido, Japan. Proceedings of the Seminar “Sea Ice Climate and Marine Environments in the Okhotsk and Baltic Seas – The Present Status and Prospects” , Seili, Finland, 10-13 September 2001. Div Geophys Univ Helsinki, 46:161–168.
Suzuki R, Ishimaru T (1990) An improved method for the determination of phytoplankton chlorophyll using N, N-dimethylformamide. J Oceanogr Soc Japan 46:190–194. https://doi.org/10.1007/BF02125580
Shirasawa K, Leppäranta M, Saloranta T et al (2005) The thickness of coastal fast ice in the Sea of Okhotsk. Cold Reg Sci Technol 42:25–40. https://doi.org/10.1016/j.coldregions.2004.11.003
Thomas DN, Lara RJ, Haas C et al (1998) Biological soup within decaying summer sea ice in the Amundsen Sea. Antarct Res Ser 73:161–171. https://doi.org/10.1029/AR073p0161
Acknowledgements
We express our heartfelt thanks to Dr. Aoki Shigeru for their support in the fieldwork. This research was supported by funds from the Japan Society for the Promotion of Science (grant numbers 15K16135 and 17H0471507), International Antarctic Institute, Institute of Low Temperature Sciences, and BEPSII (SCOR). This study is a contribution to SCOR Working Group 152 ECV-Ice (Measuring Essential Climate Variables in Sea Ice).
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Roukaerts, A., Nomura, D., Deman, F. et al. The effect of melting treatments on the assessment of biomass and nutrients in sea ice (Saroma-ko lagoon, Hokkaido, Japan). Polar Biol 42, 347–356 (2019). https://doi.org/10.1007/s00300-018-2426-y
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DOI: https://doi.org/10.1007/s00300-018-2426-y