Summary
When ice samples are melted, microorganisms living within the brine inclusions are subjected to rapid and extreme changes in salinities. This procedure results in substantial losses of flagellates and ciliates. Most of these losses can be prevented if ice samples are melted in larger volumes of sterile sea water to buffer salinity and osmotic changes. Since most studies on the ice biota have ignored, or have been unable to avoid this bias, current views of the composition and activity of sea ice communities are based on assemblages over-representing organisms with rigid cell material.
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Garrison, D.L., Buck, K.R. Organism losses during ice melting: A serious bias in sea ice community studies. Polar Biol 6, 237–239 (1986). https://doi.org/10.1007/BF00443401
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DOI: https://doi.org/10.1007/BF00443401