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An improved method for the determination of phytoplankton chlorophyll using N, N-dimethylformamide

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Abstract

Non volatile N, N-dimethylformamide (DMF) at room temperature is proposed as a new solvent for determining the chlorophylla in cultured and natural phytoplankton samples. Advantages of DMF compared to the solvents ordinarily used are (1) higher extraction efficiency, (2) rapid extraction time of 30 min, (3) simple extraction procedure requiring neither extra heating nor grinding, but soaking, and (4) a long stable life of chlorophylla (at least one month at 5°C in the dark).

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References

  • Guillard, R.R.L. and J.H. Ryther (1962): Studies of marine planktonic diatoms. I.Cyclotella nana Hustedt andDetonula confervacea (Cleve) Gran. Can. J. Microbiol.,8, 229–239.

    PubMed  Google Scholar 

  • Inskeep, W.P. and P.R. Bloom (1985): Extinction coefficients of chlorophylla andb in N, N-dimethylformamide and 80% Acetone. Plant Physiol.,77, 483–485.

    Google Scholar 

  • MacKinney, G. (1941): Absorption of light by chlorophyll solution. J. Biol. Chem.,140, 315–322.

    Google Scholar 

  • Moran, R. (1982): Formulae for determination of chlorophyllous pigments extracted with N, N-dimethylformamide. Plant Physiol.,69, 1376–1381.

    Google Scholar 

  • Moran, R. and D. Porath (1980): Chlorophyll determination in intact tissues using N, N, -dimethylformamide. Plant Physiol.,65, 478–479.

    Google Scholar 

  • Porra, R.J., W.A. Thompson, and P.E. Kriedemann (1989): Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophyllsa andb extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim. Biophys. Acta,975, 384–394.

    Google Scholar 

  • Strickland, J.D.H. and T.R. Parsons (1972): A practical handbook of seawater analysis. Bull. Fish. Res. Bd. Can.,167, 310 pp.

    Google Scholar 

  • Suzuki, R. and Y. Fujita (1986): Chlorophyll decomposition onSkeletonema costatum: a problem in chlorophyll determination of water samples. Mar. Ecol. Prog. Ser.,28, 81–85.

    Google Scholar 

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Authors and Affiliations

  1. Ocean Research Institute, University of Tokyo, Nakano-ku, 164, Tokyo, Japan

    Reiko Suzuki

  2. Tokyo University of Fisheries, Konan, Minato-ku, 108, Tokyo, Japan

    Takashi Ishimaru

Authors
  1. Reiko Suzuki
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  2. Takashi Ishimaru
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Suzuki, R., Ishimaru, T. An improved method for the determination of phytoplankton chlorophyll using N, N-dimethylformamide. Journal of the Oceanographical Society of Japan 46, 190–194 (1990). https://doi.org/10.1007/BF02125580

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  • DOI: https://doi.org/10.1007/BF02125580

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