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Halorubrum rutilum sp. nov. isolated from a marine solar saltern

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Abstract

A halophilic archaeal strain, YJ-18-S1T, was isolated from Yangjiang marine solar saltern, Guangxi Province, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-18-S1T was able to grow at 20–55 °C (optimum 37 °C), at 0.9–4.8 M NaCl (optimum 2.6 M NaCl), at 0.005–1.0 M MgCl2 (optimum 0.3 MgCl2) and at pH 5.5–8.5 (optimum pH 7.0). The cells were lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The 16S rRNA gene and rpoB′ gene of strain YJ-18-S1T were phylogenetically related to the corresponding genes of Halorubrum members (94.3–98.0 and 86.7–96.1 % similarities, respectively). The DNA G+C content of strain YJ-18-S1T was 66.2 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ-18-S1T (=CGMCC 1.12554T = JCM 30030T) represents a new species of Halorubrum, for which the name Halorubrum rutilum sp. nov. is proposed.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31370054), the 11th “Six Talents Peak” Project of Jiangsu Province (No. 2014-SWYY-021) and the Qinglan Project of Jiangsu Province and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People’s Republic of China

    Shuai Yin, Zhao Wang, Jia-Qi Xu, Wen-Mei Xu, Pan-Pan Yuan & Heng-Lin Cui

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  1. Shuai Yin
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  2. Zhao Wang
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  3. Jia-Qi Xu
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  4. Wen-Mei Xu
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Correspondence to Heng-Lin Cui.

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Communicated by Erko Stackebrandt.

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Yin, S., Wang, Z., Xu, JQ. et al. Halorubrum rutilum sp. nov. isolated from a marine solar saltern. Arch Microbiol 197, 1159–1164 (2015). https://doi.org/10.1007/s00203-015-1159-3

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