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Halomarina salina sp. nov., isolated from a marine solar saltern

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Abstract

A halophilic archaeal strain, designated ZS-57-ST, was isolated from Zhoushan marine solar saltern, China. Cells were observed to be pleomorphic, stained Gram-negative and formed red pigmented colonies on agar plates. Optimal growth was obtained at 3.9 M NaCl (range 1.4–4.8 M), 0.3 M MgCl2 (range 0–1.0 M), 30 °C (range 20–55 °C) and pH 6.5–7.5 (range 5.5–9.0). The cells were found to lyse in distilled water and the minimal NaCl concentration to prevent cell lysis was determined to be 5 % (w/v). The major polar lipids were identified as C20C20 and C20C25 diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, glucosyl mannosyl glucosyl diether and two unidentified glycolipids. The 16S rRNA gene and rpoB′ gene of strain ZS-57-ST were phylogenetically related to the corresponding genes of Halomarina oriensis JCM 16495T (98.2 and 93.7 % similarities, respectively). The DNA G+C content of strain ZS-57-ST was determined to be 67.1 mol% (T m). The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-57-ST (=CGMCC 1.12543T = JCM 30039T) represents a new species of the genus Halomarina, for which the name Halomarina salina sp. nov. is proposed.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31370054), the opening project of State key Laboratory of Microbial Resources (No. SKLMR-20150603, Institute of Microbiology, Chinese Academy of Sciences) and the 11th “Six Talents Peak” Project of Jiangsu Province (No. 2014-SWYY-021).

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  1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People’s Republic of China

    Wen-Mei Xu, Jia-Qi Xu, Yao Zhou, Yang Li, Zhen-Zhen Lü, Jing Hou, Lin Zhu & Heng-Lin Cui

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  1. Wen-Mei Xu
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  2. Jia-Qi Xu
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  3. Yao Zhou
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  7. Lin Zhu
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  8. Heng-Lin Cui
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Correspondence to Heng-Lin Cui.

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Xu, WM., Xu, JQ., Zhou, Y. et al. Halomarina salina sp. nov., isolated from a marine solar saltern. Antonie van Leeuwenhoek 109, 1121–1126 (2016). https://doi.org/10.1007/s10482-016-0714-5

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