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Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern

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Abstract

Two halophilic archaeal strains TBN4T and TBN5 were isolated from Taibei marine solar saltern in Jiangsu, China. Both strains showed light red-pigmented colonies and their cells were rod, motile and Gram-stain-negative. They were able to grow at 25–50°C (optimum 37°C), at 1.4–4.3 M NaCl (optimum 2.1 M NaCl), at 0–1.0 M MgCl2 (optimum 0.005 M MgCl2) and at pH 6.0–9.0 (optimum pH 7.0). Their cells lyse in distilled water and minimal NaCl concentration to prevent cell lysis is 8% (w/v). The major polar lipids of the two strains were PG (phosphatidylglycerol), PGP-Me (phosphatidylglycerol phosphate methyl ester), PGS (phosphatidylglycerol sulfate) and five glycolipids chromatographically identical to S-TGD-1 (sulfated galactosyl mannosyl glucosyl diether), S-DGD-1 (sulfated mannosyl glucosyl diether), TGD-1 (galactosyl mannosyl glucosyl diether), DGD-1 (mannosyl glucosyl diether) and DGD-2 (an unknown diglycosyl diether). Phylogenetic analysis revealed that TBN4T and strain TBN5 formed a distinct clade with genus Haladaptatus (showing 90.0–90.9% 16S rRNA gene similarities). The DNA G + C content of strain TBN4T and strain TBN5 are 66.1 and 65.4 mol%, respectively. The DNA–DNA hybridization value between strain TBN4T and strain TBN5 was 94.3%. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain TBN4T and strain TBN5 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halorussus rarus gen. nov., sp. nov. is proposed. The type strain is TBN4T (=CGMCC 1.10122T = JCM 16429T).

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References

  • Antón J, Oren A, Benlloch S, Rodríguez-Valera F, Amann R, Rosselló-Mora R (2002) Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. Int J Syst Evol Microbiol 52:485–491

    PubMed  Google Scholar 

  • Antunes A, Taborda M, Huber R, Moissl C, Nobre MF, da Costa MS (2008) Halorhabdus tiamatea sp. nov., a non-pigmented, extremely halophilic archaeon from a deep-sea, hypersaline anoxic basin of the Red Sea, and emended description of the genus Halorhabdus. Int J Syst Evol Microbiol 58:215–220

    Article  CAS  PubMed  Google Scholar 

  • Castillo AM, Gutiérrez MC, Kamekura M, Ma YH, Cowan DA, Jones BE, Grant WD, Ventosa A (2006) Halovivax asiaticus gen. nov., sp. nov., a novel extremely halophilic archaeon isolated from Inner Mongolia, China. Int J Syst Evol Microbiol 56:765–770

    Article  CAS  PubMed  Google Scholar 

  • Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    Article  CAS  PubMed  Google Scholar 

  • Cui HL, Lin ZY, Dong Y, Zhou PJ, Liu SJ (2007) Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 57:2204–2206

    Article  CAS  PubMed  Google Scholar 

  • Cui HL, Zhou PJ, Oren A, Liu SJ (2009) Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. Extremophiles 13:31–37

    Article  CAS  PubMed  Google Scholar 

  • Cui HL, Sun FF, Gao X, Dong Y, Xu XW, Zhou YG, Liu HC, Oren A, Zhou PJ (2010) Haladaptatus litoreus sp. nov., an extremely halophilic archaeon from a marine solar saltern, and emended description of the genus Haladaptatus. Int J Syst Evol Microbiol 60:1085–1089

    Article  CAS  PubMed  Google Scholar 

  • De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142

    Article  PubMed  Google Scholar 

  • Dussault HP (1955) An improved technique for staining red halophilic bacteria. J Bacteriol 70:484–485

    CAS  PubMed  Google Scholar 

  • Euzéby J (2010) Notification that new names and new combinations have appeared in volume 60, part 5, of the IJSEM. Int J Syst Evol Microbiol 60:1715–1716

    Article  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  CAS  PubMed  Google Scholar 

  • Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Gonzalez C, Gutierrez C, Ramirez C (1978) Halobacterium vallismortis sp. nov. an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715

    Article  CAS  PubMed  Google Scholar 

  • Gutiérrez C, González C (1972) Method for simultaneous detection of proteinase and esterase activities in extremely halophilic bacteria. Appl Microbiol 24:516–517

    PubMed  Google Scholar 

  • Gutiérrez MC, Castillo AM, Kamekura M, Ventosa A (2008) Haloterrigena salina sp. nov., an extremely halophilic archaeon isolated from a salt lake. Int J Syst Evol Microbiol 58:2880–2884

    Article  PubMed  Google Scholar 

  • Huß VAR, Festl H, Schleifer KH (1983) Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192

    Google Scholar 

  • Kates M (1986) Techniques of lipidology, 2nd edn. Elsevier, Amsterdam, pp 106–107, 241–246

  • Kumar S, Dudley J, Nei M, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Briefings Bioinf 9:299–306

    Article  CAS  Google Scholar 

  • McDade JJ, Weaver RH (1959) Rapid methods for the detection of gelatin hydrolysis. J Bacteriol 77:60–64

    CAS  PubMed  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Evol Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • Namwong S, Tanasupawat S, Visessanguan W, Kudo T, Itoh T (2007) Halococcus thailandensis sp. nov., from fish sauce in Thailand. Int J Syst Evol Microbiol 57:2199–2203

    Article  CAS  PubMed  Google Scholar 

  • Ng WL, Yang CF, Halladay JT, Arora P, DasSarma S (1995) Protocol 25: isolation of genomic and plasmid DNAs from Halobacterium halobium. In: DasSarma S, Fleischmann EM (eds) Archaea: a laboratory manual: halophiles. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 179–180

    Google Scholar 

  • Oren A (2006) The order Halobacteriales. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The prokaryotes, vol 3, 3rd edn. Springer, New York, pp 113–164

    Google Scholar 

  • Oren A, Ventosa A, Grant WD (1997) Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47:233–238

    Article  Google Scholar 

  • Oren A, Arahal DR, Ventosa A (2009) Emended descriptions of genera of the family Halobacteriaceae. Int J Syst Evol Microbiol 59:637–642

    Article  PubMed  Google Scholar 

  • Purdy KJ, Cresswell-Maynard TD, Nedwell DB, McGenity TJ, Grant WD, Timmis KN, Embley TM (2004) Isolation of haloarchaea that grow at low salinities. Environ Microbiol 6:591–595

    Article  CAS  PubMed  Google Scholar 

  • Roh SW, Nam YD, Chang HW, Sung Y, Kim KH, Lee HJ, Oh HM, Bae JW (2007) Halalkalicoccus jeotgali sp. nov., a halophilic archaeon from shrimp jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 57:2296–2298

    Article  CAS  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Savage KN, Krumholz LR, Oren A, Elshahed MS (2007) Haladaptatus paucihalophilus gen. nov., sp. nov., a halophilic archaeon isolated from a low-salt, sulfide-rich spring. Int J Syst Evol Microbiol 57:19–24

    Article  CAS  PubMed  Google Scholar 

  • Savage KN, Krumholz LR, Oren A, Elshahed MS (2008) Halosarcina pallida gen. nov., sp. nov., a halophilic archaeon from a low-salt, sulfide-rich spring. Int J Syst Evol Microbiol 58:856–860

    Article  PubMed  Google Scholar 

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  • Wang QF, Li W, Yang H, Liu YL, Cao HH, Dornmayr-Phaffenhuemer M, Stan-Lotter H, Guo GQ (2007) Halococcus qingdaonensis sp. nov., a halophilic archaeon isolated from a crude sea-salt sample. Int J Syst Evol Microbiol 57:600–604

    Article  CAS  PubMed  Google Scholar 

  • Xin H, Itoh T, Zhou P, Suzuki K, Kamekura M, Nakase T (2000) Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50:1297–1303

    CAS  PubMed  Google Scholar 

  • Xu Y, Zhou P, Tian X (1999) Characterization of two novel haloalkaliphilic archaea Natronorubrum bangense gen. nov., sp. nov. and Natronorubrum tibetense gen. nov., sp. nov. Int J Syst Bacteriol 49:261–266

    Article  CAS  PubMed  Google Scholar 

  • Xu XW, Wu YH, Wang CS, Oren A, Zhou PJ, Wu M (2007) Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 57:717–720

    Article  CAS  PubMed  Google Scholar 

  • Xue YF, Fan HP, Ventosa A, Grant WD, Jones BE, Cowan DA, Ma YH (2005) Halalkalicoccus tibetensis gen. nov., sp. nov., representing a novel genus of haloalkaliphilic archaea. Int J Syst Evol Microbiol 55:2501–2505

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30970006) and the opening project of State key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (No. SKLMR-20100604).

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Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People’s Republic of China

    Heng-Lin Cui, Xia Gao & Xin Yang

  2. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Heng-Lin Cui

  3. Second Institute of Oceanography, State Oceanic Administration, Hangzhou, 310012, People’s Republic of China

    Xue-Wei Xu

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  1. Heng-Lin Cui
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  2. Xia Gao
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Correspondence to Heng-Lin Cui.

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Communicated by A. Oren.

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Cui, HL., Gao, X., Yang, X. et al. Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. Extremophiles 14, 493–499 (2010). https://doi.org/10.1007/s00792-010-0329-0

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