Skip to main content
SpringerLink
Log in

Halorubrum laminariae sp. nov., isolated from the brine of salted brown alga Laminaria

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

Two halophilic archaeal strains, R60T and R61, were isolated from the brine of salted brown alga Laminaria. Cells of the two strains were observed to be rod-shaped, stain Gram-negative and to lyse in distilled water. Strain R60T was found to contain gas vacuoles and to produce pink-pigmented colonies, while strain R61 lacked gas vacuoles and produces red-pigmented colonies. Both strains were found to be able to grow at 20–50 °C (optimum 30 °C), at 1.7–4.8 M NaCl (optimum 2.6–3.1 M NaCl), at 0–1.0 M MgCl2 (optimum 0.005–0.1 M MgCl2) and at pH 6.0–9.5 (optimum pH 7.0). The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to a sulfated mannosyl glucosyl diether produced by Halorubrum members of the Halobacteriaceae. The 16S rRNA gene sequences of the two strains were 99.9 % identical, showing 94.6–98.0 % similarity to those of members of the genus Halorubrum. The EF-2 gene similarity between strains R60T and R60 was 100 % and showed 84.6–94.5 % similarity to those of members of the genus Halorubrum. The DNA G+C contents of the two strains were determined to be 63.0 mol %. The DNA–DNA hybridization value between strain R60T and strain R61 was 92 % and the two strains showed low DNA–DNA relatedness with the most related members of Halorubrum. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain R60T (= CGMCC 1.12689T = JCM 30040T) and strain R61 (= CGMCC 1.12696) represent a novel species of the genus Halorubrum, for which the name Halorubrum laminariae sp. nov. is proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Abriouel H, Benomar N, Lucas R, Gálvez A (2011) Culture-independent study of the diversity of microbial populations in brines during fermentation of naturally-fermented Aloreña green table olives. Int J Food Microbiol 144:487–496

    Article  CAS  PubMed  Google Scholar 

  • Cui HL, Lin ZY, Dong Y, Zhou PJ, Liu SJ (2007) Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 57:2204–2206

    Article  CAS  PubMed  Google Scholar 

  • Cui HL, Zhou PJ, Oren A, Liu SJ (2009) Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. Extremophiles 13:31–37

    Article  CAS  PubMed  Google Scholar 

  • Cui HL, Gao X, Yang X, Xu XW (2010) Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. Extremophiles 14:493–499

    Article  CAS  PubMed  Google Scholar 

  • Cui HL, Yang X, Mou YZ (2011) Salinarchaeum laminariae gen. nov., sp. nov.: a new member of the family Halobacteriaceae isolated from salted brown alga Laminaria. Extremophiles 15:625–631

    Article  CAS  PubMed  Google Scholar 

  • De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142

    Article  PubMed  Google Scholar 

  • Dussault HP (1955) An improved technique for staining red halophilic bacteria. J Bacteriol 70:484–485

    CAS  PubMed Central  PubMed  Google Scholar 

  • Gonzalez C, Gutierrez C, Ramirez C (1978) Halobacterium vallismortis sp. nov. an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715

    Article  CAS  PubMed  Google Scholar 

  • Gutiérrez C, González C (1972) Method for simultaneous detection of proteinase and esterase activities in extremely halophilic bacteria. Appl Microbiol 24:516–517

    PubMed Central  PubMed  Google Scholar 

  • Han D, Cui HL (2014a) Halosimplex pelagicum sp. nov. and Halosimplex rubrum sp. nov., isolated from salted brown alga Laminaria, and emended description of the genus Halosimplex. Int J Syst Evol Microbiol 64:169–173

    Article  PubMed  Google Scholar 

  • Han D, Cui H-L (2014b) Haloplanus litoreus sp. nov. and Haloplanus ruber sp. nov., from a marine solar saltern and an aquaculture farm, respectively. Antonie Van Leeuwenhoek 105:679–685

    Article  CAS  PubMed  Google Scholar 

  • Huß VAR, Festl H, Schleifer KH (1983) Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192

    Article  PubMed  Google Scholar 

  • Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118

    Article  CAS  PubMed  Google Scholar 

  • McDade JJ, Weaver RH (1959) Rapid methods for the detection of gelatin hydrolysis. J Bacteriol 77:60–64

    CAS  PubMed Central  PubMed  Google Scholar 

  • McGenity TJ, Grant WD (1995) Transfer of Halobacterium saccharovorum, Halobacterium sodomense, Halobacterium trapanicum NRC 34021 and Halobacterium lacusprofundi to the genus Halorubrum gen. nov., as Halorubrum saccharovorum comb. nov., Halorubrum sodomense comb. nov., Halorubrum trapanicum comb. nov., and Halorubrum lacusprofundi comb. nov. Syst Appl Microbiol 18:237–243

    Article  Google Scholar 

  • Minegishi H, Kamekura M, Itoh T, Echigo A, Usami R, Hashimoto T (2010) Further refinement of Halobacteriaceae phylogeny based on the full-length RNA polymerase subunit B’ (rpoB ’) gene. Int J Syst Evol Microbiol 60:2398–2408

    Article  PubMed  Google Scholar 

  • Mou YZ, Qiu XX, Zhao ML, Cui HL, Oh D, Dyall-Smith ML (2012) Halohasta litorea gen. nov. sp. nov., and Halohasta litchfieldiae sp. nov., isolated from the Daliang aquaculture farm, China and from Deep Lake, Antarctica, respectively. Extremophiles 16:895–901

    Article  CAS  PubMed  Google Scholar 

  • Mwatha WE, Grant WD (1993) Natronobacterium vacuolata sp. nov., a haloalkaliphilic archaeon isolated from Lake Magadi, Kenya. Int J Syst Bacteriol 43:401–404

    Article  Google Scholar 

  • Oren A, Ventosa A, Grant WD (1997) Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47:233–238

    Article  Google Scholar 

  • Oren A, Arahal DR, Ventosa A (2009) Emended descriptions of genera of the family Halobacteriaceae. Int J Syst Evol Microbiol 59:637–642

    Article  PubMed  Google Scholar 

  • Qiu X-X, Zhao M-L, Han D, Zhang W-J, Dyall-Smith ML, Cui H-L (2013) Taxonomic study of the genera Halogeometricum and Halosarcina: transfer of Halosarcina limi and Halosarcina pallida to the genus Halogeometricum as Halogeometricum limi comb. nov. and Halogeometricum pallidum comb. nov., respectively. Int J Syst Evol Microbiol 63:3915–3919

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Yim KJ, Cha I-T, Lee H-W, Song HS, Kim K-N, Lee S-J, Nam Y-D, Hyun D-W, Bae J-W, Rhee S-K, Seo M-J, Choi J-S, Choi H-J, Roh SW, Kim D (2014) Halorubrum halophilum sp. nov., an extremely halophilic archaeon isolated from a salt-fermented seafood. Antonie Van Leeuwenhoek 105:603–612

    Article  CAS  PubMed  Google Scholar 

  • Zhang W-J, Cui H-L (2014) Halorubrum salinum sp. nov., isolated from a marine solar saltern. Arch Microbiol 196:395–400

    Article  CAS  PubMed  Google Scholar 

  • Zhaxybayeva O, Stepanauskas R, Mohan NR, Papke RT (2013) Cell sorting analysis of geographically separated hypersaline environments. Extremophiles 17:265–275

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31370054), the grant from China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (DY125-15-R-03), the Qinglan Project of Jiangsu province and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Author information

Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People’s Republic of China

    Dong Han & Heng-Lin Cui

Authors
  1. Dong Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Heng-Lin Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Heng-Lin Cui.

Electronic supplementary material

Phase-contrast micrographs of strains R60T and R61, thin-layer chromatograms of strains R60T, R61, Halorubrum saccharovorum CGMCC 1.2147T and Halorubrum aidingense CGMCC 1.2670T, are available as supplementary materials.

Supplementary material 1 (DOC 2805 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Han, D., Cui, HL. Halorubrum laminariae sp. nov., isolated from the brine of salted brown alga Laminaria . Antonie van Leeuwenhoek 107, 217–223 (2015). https://doi.org/10.1007/s10482-014-0319-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-014-0319-9

Keywords

Search

Navigation