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Halobium Salinum sp. nov., Isolated from a Marine Solar Saltern

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Abstract

A halophilic archaeal strain YJ-8-ST was isolated from Yangjiang marine solar saltern, China. Cells from strain YJ-8-ST were pleomorphic, lysed in distilled water, stained Gram-negative, and formed red-pigmented colonies on agar plate. Optimal growth of the strain was obtained at 3.1 M NaCl (range 1.4–4.8 M), 0.1 M MgCl2 (range 0.005–1.0 M), 37 °C (range 20–50 °C), and pH 7.5 (range 5.5–9.5). The major polar lipids are phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1), and mannosyl glucosyl diether (DGD-1). The 16S rRNA gene and rpoB′ gene of strain YJ-8-ST were phylogenetically related to the corresponding genes of Halobium palmae (96.9–97.2 and 92.7% similarities, respectively). The DNA G+C content of strain YJ-8-ST was determined to be 68.9 mol%. The phenotypic, chemotaxonomic, and phylogenetic characteristics suggested that strain YJ-8-ST (=CGMCC 1.12553T = JCM 30029T) represents a new species of Halobium, for which the name Halobium salinum sp. nov. is proposed.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31370054) and the 11th “Six Talents Peak” Project of Jiangsu Province (No. 2014-SWYY-021). We are grateful to Dr. Koji Mori (NITE Biological Resource Center, Japan) for kindly providing the type strain (Halobium palmae NBRC 111368T).

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Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People’s Republic of China

    Yang Li, Yao Zhou, Zhen-Zhen Lü & Heng-Lin Cui

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  1. Yang Li
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  2. Yao Zhou
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  3. Zhen-Zhen Lü
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  4. Heng-Lin Cui
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Correspondence to Heng-Lin Cui.

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Additional information

The Digital Protologue database Taxon Number for strain YJ-8-ST is TA00230. The GenBank/EMBL/DDBJ accession numbers for the rrnA and rrnB of 16S rRNA, and rpoB′ gene sequences of strain YJ-8-ST are KC918818, KX171225, and KX171226, respectively.

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Li, Y., Zhou, Y., Lü, ZZ. et al. Halobium Salinum sp. nov., Isolated from a Marine Solar Saltern. Curr Microbiol 75, 6–10 (2018). https://doi.org/10.1007/s00284-017-1343-6

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  • DOI: https://doi.org/10.1007/s00284-017-1343-6

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