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Molecular characterization of lipoxygenase genes on chromosome 4BS in Chinese bread wheat (Triticum aestivum L.)

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This study cloned two novel TaLox genes on chromosome of 4BS and developed a co-dominant marker, Lox-B23, in bread wheat that showed highly significant association with lipoxygenase activity.

Abstract

Lipoxygenase (Lox), a critical enzyme in the carotenoid biosynthetic pathway, significantly influences the color and processing quality of wheat-based products. Two novel Lox genes, designated TaLox-B2 and TaLox-B3, were cloned on chromosome 4BS of Chinese bread wheat. The deduced amino acid sequence showed that both TaLox-B2 and TaLox-B3 genes encoded an 861-aa protein and possessed a lipoxygenase superfamily domain at the 170–838 interval. Two different TaLox-B2 alleles, designated TaLox-B2a and TaLox-B2b, were subsequently discovered. A co-dominant marker, Lox-B23, was developed based on sequences of TaLox-B2a, TaLox-B2b, and TaLox-B3 genes to precisely distinguish these three alleles in Chinese bread cultivars. Among five allelic combinations of Lox genes at Lox-B1, Lox-B2, and Lox-B3 loci, wheat cultivars with TaLox-B1a/TaLox-B2a/TaLox-B3a combination exhibited the highest Lox activity, whereas those with TaLox-B1a/TaLox-B2b/TaLox-B3b combination significantly showed the lowest Lox activity. A RIL population was used to evaluate the influence of TaLox-B3a gene on Lox activity. Results showed that TaLox-B3a gene could significantly increase the Lox activity in bread wheat. Physical mapping indicated that both TaLox-B2 and TaLox-B3 genes were located on chromosome 4BS in bread wheat. This study provides useful information to further understand the molecular and genetic bases of Lox activity in bread wheat.

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Acknowledgments

This project was funded by the 973 projects (2014CB138105 and 2014CB160303), National Natural Science Foundation (31370031) and Program for New Century Excellent Talents in University (NCET-13-0776) of China.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Collaborative Innovation Center of Henan Grain Crops, Zhengzhou, 450002, China

    Fuyan Zhang, Feng Chen, Peipei Wu, Ning Zhang & Dangqun Cui

  2. National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450002, China

    Feng Chen & Dangqun Cui

  3. Agronomy College, Henan Agricultural University, Zhengzhou, 450002, China

    Feng Chen & Dangqun Cui

Authors
  1. Fuyan Zhang
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  2. Feng Chen
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  5. Dangqun Cui
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Corresponding authors

Correspondence to Feng Chen or Dangqun Cui.

Additional information

Communicated by I. D. Godwin.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplemental 1. Name, origin and TaLox-Bgenotype of Chinese bread wheat cultivars surveyed (XLSX 15 kb)

122_2015_2518_MOESM2_ESM.docx

Supplemental 2. Full alignment of genomic DNA sequences of TaLox-B2a, TaLox-B2b and TaLox-B3 alleles in bread wheat (DOCX 26 kb)

Supplemental 3. Polygenetic tree of different lipoxygenase genes from tetraploid and hexaploid wheat (TIFF 139 kb)

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Zhang, F., Chen, F., Wu, P. et al. Molecular characterization of lipoxygenase genes on chromosome 4BS in Chinese bread wheat (Triticum aestivum L.). Theor Appl Genet 128, 1467–1479 (2015). https://doi.org/10.1007/s00122-015-2518-9

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  • DOI: https://doi.org/10.1007/s00122-015-2518-9

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