Abstract
OsLOX1 is a novel full-length cDNA isolated from developing rice seeds. We have examined its biochemical properties and expression patterns. The protein has dual positional specificity, as it releases both C-9 and C-13 oxidized products in a 4:3 ratio. OsLOX1 transcripts were detected at low abundance in immature seeds and newly germinated seedlings, but accumulate rapidly and transiently in response to wounding or brown planthopper (BPH) attack, reaching a peak 3 h after wounding and 6 h after insect feeding. We produced transgenic rice lines carrying either sense or antisense constructs under the control of a cauliflower mosaic virus 35S promoter, and these rice lines showed altered OsLOX1 activity. In all of the antisense lines and more than half of the sense lines the expression levels of OsLOX1, the levels of enzyme activity, and the levels of the endogenous OsLOX1 products (jasmonic acid, (Z)-3-hexenal and colneleic acid) at 6, 48, and 48 h after BPH feeding respectively, were below the levels found in non-transgenic control plants; yet, the levels in the remaining sense transformants were enhanced relative to controls. Transformants with a lower level of OsLOX1 expression were less able to tolerate BPH attack, while those with enhanced OsLOX1 expression were more resistant. Our data suggest that the OsLOX1 product is involved in tolerance of the rice plant to wounding and BPH attack.
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Abbreviations
- LOX:
-
Lipoxygenase
- UTR:
-
Untranslated region
- BPH:
-
Brown planthopper
- BTH:
-
Benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester
- CA:
-
Colneleic acid
- ORF:
-
Open reading frame
- JA:
-
Jasmonic acid
- HPOD:
-
Hydroperoxyoctadecadienoic acid
- GFP:
-
Green fluorescent protein
- RACE:
-
Rapid amplification of cDNA end
- HPLC:
-
High performance liquid chromatography
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Acknowledgements
This work was supported by the National Key Basic Research “973” Program of China (Grant No. 2004CB117204), by the National “863” program (Grant No. 2006AA100101, 2006AA10Z1A5 and 2006AA10A102), by the National Natural Science Foundation of China (Grant No. 30270817), and by the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT). We owe special thanks to Prof. Bing Xia and Dr. Yu Shan at Institute of Botany Jiangsu Province & Chinese Academy of Sciences for assaying levels of JA and (Z)-3-hexenal; we also thank Dr. Genji Qin from the College Life Sciences at Peking University for assistance in northern blot analysis, and Prof. Shihu Yang in Nanjing Agricultural University for providing the rice transformation vectors pPZP2Ha3(+) and pPZP2Ha3(-).
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Wang, R., Shen, W., Liu, L. et al. A novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack. Plant Mol Biol 66, 401–414 (2008). https://doi.org/10.1007/s11103-007-9278-0
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DOI: https://doi.org/10.1007/s11103-007-9278-0