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In Vitro Conservation and Cryopreservation of Clonally Propagated Horticultural Species

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Conservation and Utilization of Horticultural Genetic Resources

Abstract

Rapid strides have been made in plant biotechnology, including plant tissue culture and conservation techniques. The newly acquired knowledge is now being commonly applied in collection, exchange, multiplication and short- to long-term conservation of genetic resources of crops, especially horticultural crops. Several protocols for in vitro conservation and/or cryopreservation are reported in both temperate and tropical species of fruits, spices, tubers and ornamental and medicinal species. Techniques for rapid multiplication and production of plant material using tissue culture methods are important prerequisites for a successful programme on in vitro conservation and cryopreservation. In case of clones to be maintained under conservation, monitoring genetic stability becomes an integral activity to ensure avoidance of somaclonal variation. In vitro conservation of horticultural germplasm has been successfully applied by means of in vitro slow growth storage in several national and international gene banks/laboratories. Cryopreservation of germplasm (normally using liquid nitrogen, −196 °C) is currently advocated as the safest and cost-effective long-term strategy for conservation of horticultural genetic resources. Although large-scale, routine application of cryopreservation is still limited to a few laboratories, there are an ever-increasing number of publications reporting successful protocols in many horticultural species. This has been possible due to advances in development of more ‘generic’ new-age cryopreservation protocols like droplet-vitrification.

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Acknowledgements

The authors are grateful to the Director(s) and all staff of NBPGR/TCCU who have contributed towards the above-mentioned activities. All photographs used in the chapter are copyright of ICAR-NBPGR.

Disclaimer: The views, opinions or recommendations expressed are strictly those of the authors and do represent those of the organization they are affiliated to.

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Authors and Affiliations

  1. ICAR-National Bureau of Plant Genetic Resources, New Delhi, India

    Anuradha Agrawal, Shivani Singh, Era Vaidya Malhotra & D. P. S. Meena

  2. Asia-Pacific Consortium on Agricultural Biotechnology and Bioresources, Asia-Pacific Association of Agricultural Research Institutions, Bangkok, Thailand

    R. K. Tyagi

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  1. Anuradha Agrawal
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  2. Shivani Singh
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  3. Era Vaidya Malhotra
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  4. D. P. S. Meena
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Correspondence to Anuradha Agrawal .

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  1. Division of Plant Genetic Resources, ICAR-Indian Institute of Horticultural Research, Bengaluru, Karnataka, India

    P.E. Rajasekharan

  2. Global Research for Development Support Ventures (GRSV), Bengaluru, Karnataka, India

    V. Ramanatha Rao

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Agrawal, A., Singh, S., Malhotra, E.V., Meena, D.P.S., Tyagi, R.K. (2019). In Vitro Conservation and Cryopreservation of Clonally Propagated Horticultural Species. In: Rajasekharan, P., Rao, V. (eds) Conservation and Utilization of Horticultural Genetic Resources. Springer, Singapore. https://doi.org/10.1007/978-981-13-3669-0_18

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