Abstract
Nodal segments of Hibiscus moscheutos (hardy hibiscus) were excised from proliferating axillary shoot cultures and encapsulated in high density sodium alginate hardened by 50 mM CaCl2. Nodal segments 4 mm long grew as well as and were easier to encapsulate than 8 mm long nodal segments. Although nodal segments grew regardless of the concentration of sodium alginate, 2.75% was determined to produce the highest quality encapsulated nodal segments beads (sufficient alginate coating and ease of use) because of the viscosity produced by the 2.75% sodium alginate solution. When encapsulated segments were stored at 5°C they did not grow in light or darkness. During the first month on fresh proliferation medium under normal incubation conditions following 5°C storage in the dark for up to 24 weeks, root number and root and shoot elongation were inhibited linearly as storage time increased. All encapsulated nodal segments survived 24 weeks of 5°C storage in two separate experiments. In fact, 80% of encapsulated hardy hibiscus nodal segments survived refrigerated storage for 1½ years (78 weeks) and after 3 months on proliferation medium, the nodal segments produced nearly the same length axillary shoots with the same number of axillary nodes per shoot as compared to encapsulated segments either not stored at 5°C or stored for 24 weeks at 5°C. Growth from encapsulated and cold-stored ‘Lord Baltimore’ nodal segments was more vigorous than from ‘Southern Belle’ nodal segments.
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Ballester A, Janeiro LV, Vieitez AM (1997) Cold storage of shoot cultures and alginate encapsulation of shoot tips of Camellia japonica L. and Camellia reticulata Lindley. Sci Hort 71:67–78
Driver JA, Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock. Hortscience 19:507–509
Miaja ML, Gribaudo I, Vallania R, Fernandez LF (2000) Low temperature storage and cryopreservation of a Vitis vinifera L. germplasm collection: first results. Acta Hort 538:177–181
Monette PL (1987) Organogenesis and plantlet regeneration following in vitro cold storage of kiwifruit shoot tip cultures. Sci Hortic 31:101–106
Orlikowska T (1992) Effect of in vitro storage at 4°C on surviving and proliferation of two apple rootstocks. Plant Cell Tiss Org Cult 31:1–7
Piccioni E, Standardi A (1995) Encapsulation of micropropagated buds of six woody species. Plant Cell Tiss Org Cult 42:221–226
Standardi A, Piccioni E (1998) Recent perspectives on synthetic seed technology using nonembryogenic in vitro-derived explants. Int J Plant Sci 159(6):968–978
Steel RGD, Torrie JH (1980) Principles and Procedures of Statistics. In: A biometrical approach, 2nd edn. McGraw-Hill, New York
Timbert R, Barbotin J-N, Kersulec A, Bazinet C, Thomas D (1995) Physico-chemical properties of the encapsulation matrix and germination of carrot somatic embryos. Biotechnol Bioeng 46:573–578
West TP, Preece JE (2004) Effects of thidiazuron and nutrient salt formulations on micropropagation of hardy hibiscus (Hibiscus moscheutos L.). Acta Hort 630:293–297
West TP, Preece JE (2006) Use of acephate, benomyl and alginate encapsulation for eliminating culture mites and fungal contamination from in vitro cultures of hardy hibiscus (Hibiscus moscheutos L.). In Vitro Cell Dev Biol Plant 42:301–304
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West, T.P., Ravindra, M.B. & Preece, J.E. Encapsulation, cold storage, and growth of Hibiscus moscheutos nodal segments. Plant Cell Tiss Organ Cult 87, 223–231 (2006). https://doi.org/10.1007/s11240-006-9155-6
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DOI: https://doi.org/10.1007/s11240-006-9155-6