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In vitro propagation of Allium tuberosum Rottl. ex. Spreng. by shoot proliferation

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Abstract

Seeds of trifoliate orange (Poncirus trifoliata (L.) Raf.) are sensitive to desiccation, and could not withstand reduction in moisture level below 20%, whereas the excised embryonic axes could be easily desiccated to moisture levels as low as 14% without much loss in viability. Axes could be successfully cryopreserved in liquid nitrogen (−196°C) for eight months. The viable embryonic axes exhibited good growth on modified Murashige and Skoog medium supplemented with 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP). Growth of cryopreserved axes was promoted in the presence of charcoal in the medium allowing for plant recovery.

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Abbreviations

NAA:

Napthaleneacetic acid

BAP:

6-Benzylamino-purine

MS:

Murashige and Skoog (1962)

LN:

Liquid nitrogen

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Authors and Affiliations

  1. National Plant Tissue Culture Repository, NBPGR Campus, 110012, New Delhi, India

    Ruchira Pandey, K. P. S. Chandel & S. Rama Rao

Authors
  1. Ruchira Pandey
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  2. K. P. S. Chandel
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  3. S. Rama Rao
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Additional information

Communicated by G. C. Phillips

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Pandey, R., Chandel, K.P.S. & Rama Rao, S. In vitro propagation of Allium tuberosum Rottl. ex. Spreng. by shoot proliferation. Plant Cell Reports 11, 211–214 (1992). https://doi.org/10.1007/BF00232536

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  • DOI: https://doi.org/10.1007/BF00232536

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