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Advances in NMR Data Acquisition and Processing for Protein Structure Determination

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Experimental Approaches of NMR Spectroscopy

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Abstract

Solution NMR has become an indispensable technique in studying the physical properties and functions of biomacromolecules at near physiological conditions. However, biomolecular systems are often extraordinarily large, dynamic, and possess low solubility, which complicates the acquisition and analysis of NMR spectra. Together this results in severe signal overlapping and low signal to noise, which demands longer total experimental time. NMR spectroscopists must consider how to improve spectral resolution and sensitivity to extract the maximum structural information from imperfect data within reasonable measurement times. Recent advances in multi-dimensional NMR acquisition and computation methods overcome these problems and enable the investigation of larger and more complicated biomolecular systems. This chapter first reviews conventional and then state-of-the-art methodologies for NMR data collection, signal processing, and protein structure data analysis. In the first section, we survey the basics of multi-dimensional NMR and present rapid measurement techniques such as non-uniform sampling and spectrum reconstruction algorithms. In the second half, we illustrate the conventions of protein structure determination using chemical shift and NOE data to obtain interatomic distances, dihedral angles, and dynamics information. We also briefly introduce the currently popular hybridisation of NMR with solution angle scattering for 3D structure analysis.

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Acknowledgements

The authors thank Dr. Youhei Kawabata for useful discussions regarding solution scattering and Dr. Joshua James Ziarek and Prof. Peter Güntert for a critical reading of this manuscript. We gratefully acknowledge financial supports by Scientific Research on Innovative Areas (JP26102538, JP25120003, JP16H00779 to T.I., and JP15H01645, JP16H00847 to Y.I.) and Grants-in-Aid for Scientific Research (JP15K06979 to T.I.) from the Japan for the Promotion of Science (JSPS) and by the Funding Program for Core Research for Evolutional Science and Technology (CREST JPMJCR13M3) from Japan Science and Technology Agency (JST).

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    Teppei Ikeya & Yutaka Ito

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Ikeya, T., Ito, Y. (2018). Advances in NMR Data Acquisition and Processing for Protein Structure Determination. In: The Nuclear Magnetic Resonance Society of Japan (eds) Experimental Approaches of NMR Spectroscopy. Springer, Singapore. https://doi.org/10.1007/978-981-10-5966-7_3

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