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SOFAST-HMQC Experiments for Recording Two-dimensional Deteronuclear Correlation Spectra of Proteins within a Few Seconds

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Abstract

Fast multidimensional NMR with a time resolution of a few seconds provides a new tool for high throughput screening and site-resolved real-time studies of kinetic molecular processes by NMR. Recently we have demonstrated the feasibility to record protein 1H–15N correlation spectra in a few seconds of acquisition time using a new SOFAST-HMQC experiment (Schanda and Brutscher (2005) J. Am. Chem. Soc. 127, 8014). Here, we investigate in detail the performance of SOFAST-HMQC to record 1H–15N and 1H−13C correlation spectra of proteins of different size and at different magnetic field strengths. Compared to standard 1H–15N correlation experiments SOFAST-HMQC provides a significant gain in sensitivity, especially for fast repetition rates. Guidelines are provided on how to set up SOFAST-HMQC experiments for a given protein sample. In addition, an alternative pulse scheme, IPAP-SOFAST-HMQC is presented that allows application on NMR spectrometers equipped with cryogenic probes, and fast measurement of one-bond 1H–13C and 1H–15N scalar and residual dipolar coupling constants.

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Authors and Affiliations

  1. Institut de Biologie Structurale – Jean-Pierre Ebel, UMR5075 CNRS-CEA-UJF, 41, rue Jules Horowitz, 38027, Grenoble Cedex, France

    Paul Schanda & Bernhard Brutscher

  2. Varian Ltd., Walton-on-Thames, Surrey, KT12 2QF, United Kingdom

    Ēriks Kupče

Authors
  1. Paul Schanda
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  2. Ēriks Kupče
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  3. Bernhard Brutscher
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Correspondence to Bernhard Brutscher.

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Schanda, P., Kupče, Ē. & Brutscher, B. SOFAST-HMQC Experiments for Recording Two-dimensional Deteronuclear Correlation Spectra of Proteins within a Few Seconds. J Biomol NMR 33, 199–211 (2005). https://doi.org/10.1007/s10858-005-4425-x

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  • DOI: https://doi.org/10.1007/s10858-005-4425-x

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