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Metabolic Engineering for the Biosynthesis of Terpenoids from Microbial Cell Factories

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Microbial Bioactive Compounds

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Abstract

Terpenes, terpenoids, or isoprenoids are the largest class of secondary metabolites produced by the natural system. Since ages the plants are being utilized as a source of flavor, fragrance, and therapeutics. Due to their higher energy density and other superior fuel properties, terpenoids are also foreseen as potential alternative to petroleum-derived fuels. The current market demand of terpenoids is fulfilled either by their plant-based extraction or by chemical synthesis. However, both the mythologies have their own set of limitations. Therefore, from the past two decades, the global research interest on terpenoid production has been shifted toward their biosynthesis from microbial routes. Microbes have provided an excellent platform for the biosynthesis of commodity chemicals due to their several advantages over plants, such as fastidious growth, low space and seasonal requirements, and simple growth medium. Moreover, the genetic modification in microbes can be done with a high success rate as compared to plants. However, microbes are not the natural producers of the majority of industrially important terpenoids. Therefore, they need to be engineered for the non-natural production of terpenoids. Advancement of synthetic and metabolic engineering approaches has enabled researchers to incorporate entire novel pathways and silence native pathways of the host microbes at the same time. Thus, provided an excellent platform not only for the non-native production of terpenoids but also improving the titers of the desired metabolites from engineered microbes. In this present chapter, we have summarized the metabolic engineering approaches for the sustainable production of terpenoid-based metabolites for microbial sources, with an emphasis on prokaryotic host organisms.

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Authors and Affiliations

  1. Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research, Lucknow, India

    Vibha Shukla

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India

    Vibha Shukla

  3. Department of Respiratory Medicine, King George Medical University, Lucknow, India

    Parul Gupta

  4. Department of Biotechnology, Govind Ballabh Pant Institute of Engineering and Technology, Ghurdauri, Pauri Garhwal, Uttarakhand, India

    Suresh Chandra Phulara

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  1. Vibha Shukla
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  2. Parul Gupta
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  3. Suresh Chandra Phulara
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Editors and Affiliations

  1. Department of Agricultural Microbiology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, India

    Ravindra Soni

  2. Vidyadayini Institute of Science, Management and Technology, Bhopal, India

    Deep Chandra Suyal

  3. Facultad de Ciencias Quimicas, Universidad Autonoma de Coahuila, Saltillo, Mexico

    Lourdes Morales-Oyervides

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Shukla, V., Gupta, P., Phulara, S.C. (2023). Metabolic Engineering for the Biosynthesis of Terpenoids from Microbial Cell Factories. In: Soni, R., Suyal, D.C., Morales-Oyervides, L. (eds) Microbial Bioactive Compounds. Springer, Cham. https://doi.org/10.1007/978-3-031-40082-7_4

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