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Biosynthesis of zeaxanthin in recombinant Pseudomonas putida

  • Applied Genetics and Molecular Biotechnology
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Abstract

Pseudomonas putida KT2440 strain was investigated for biosynthesis of the valuable xanthophyll zeaxanthin. A new plasmid was constructed harboring five carotenogenic genes from Pantoea ananatis and three genes from Escherichia coli under control of an l-rhamnose-inducible promoter. Pseudomonas putida KT2440 wild type hardly tolerated the plasmids for carotenoid production. Mating experiments with E. coli S17-1 strains revealed that the carotenoid products are toxic to the Pseudomonas putida cells. Several carotenoid-tolerant transposon mutants could be isolated, and different gene targets for relief of carotenoid toxicity were identified. After optimization of cultivation conditions and product processing, 51 mg/l zeaxanthin could be produced, corresponding to a product yield of 7 mg zeaxanthin per gram cell dry weight. The effect of various additives on production of hydrophobic zeaxanthin was investigated as well. Particularly, the addition of lecithin during cell cultivation increased volumetric productivity of Pseudomonas putida by a factor of 4.7 (51 mg/l vs. 239 mg/l).

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Acknowledgment

The authors want to thank the Institute of Food Chemistry, Universität Hohenheim for the carotenoids and wolfberries. The authors further thank the Landesstiftung Baden-Württemberg for the financial support.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute of Technical Biochemistry, Universitaet Stuttgart, Allmandring 31, 70569, Stuttgart, Germany

    Holger Beuttler, Bernhard Hauer, Rolf D. Schmid & Vlada B. Urlacher

  2. Institute of Industrial Genetics, Universitaet Stuttgart, Allmandring 31, 70569, Stuttgart, Germany

    Jana Hoffmann, Marcel Jeske & Josef Altenbuchner

  3. Institute of Biochemistry, Heinrich-Heine-University Düsseldorf, Universitätsstr.1, 40225, Düsseldorf, Germany

    Vlada B. Urlacher

Authors
  1. Holger Beuttler
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  2. Jana Hoffmann
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  3. Marcel Jeske
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  4. Bernhard Hauer
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  5. Rolf D. Schmid
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  6. Josef Altenbuchner
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  7. Vlada B. Urlacher
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Correspondence to Vlada B. Urlacher.

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Beuttler, H., Hoffmann, J., Jeske, M. et al. Biosynthesis of zeaxanthin in recombinant Pseudomonas putida . Appl Microbiol Biotechnol 89, 1137–1147 (2011). https://doi.org/10.1007/s00253-010-2961-0

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  • DOI: https://doi.org/10.1007/s00253-010-2961-0

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