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Chromobacterium amazonense sp. nov. isolated from water samples from the Rio Negro, Amazon, Brazil

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Abstract

The taxonomic position of a bacterium isolated from water samples from the Rio Negro, in Amazon, Brazil, was determined by using a polyphasic approach. The organism formed a distinct phyletic line in the Chromobacterium 16S rRNA gene tree and had chemotaxonomic and morphological properties consistent with its classification in this genus. It was found to be closely related to Chromobacterium vaccinii DSM 25150T (98.6 % 16S rRNA gene similarity) and shared 98.5 % 16S rRNA gene similarity with Chromobacterium piscinae LGM 3947T. DNA–DNA relatedness studies showed that isolate CBMAI 310T belongs to distinct genomic species. The isolate was readily distinguished from the type strain of these species using a combination of phenotypic and chemotaxonomic properties. Thus, based on genotypic and phenotypic data, it is proposed that isolate CBMAI 310T (=DSM 26508T) be classified in the genus Chromobacterium as the type strain of a novel species, namely, Chromobacterium amazonense sp. nov.

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Acknowledgments

We thank Spartaco Astolfi Filho at the Universidade Federal do Amazonas, Brazil, by deposited of Chrombacterium sp strains for the Industrial and Environmental Microorganisms Brazilian Collection (CBMAI). This research was kindly supported by a grant from the 2005/60852-4, São Paulo Research Foundation (FAPESP) and National Council for Scientific and Technological Development (CNPq).

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Authors and Affiliations

  1. Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, UNICAMP, Avenida Alexandre Cazelatto, 999, Campinas, SP, CEP 13083-218, Brazil

    Claudia Beatriz Afonso Menezes & Fabiana Fantinatti-Garboggini

  2. Programa de Pós-Graduação Interunidades em Biotecnologia, USP, Avenida Lineu Prestes, 2415, ICB III, São Paulo, SP, CEP 05508-900, Brazil

    Claudia Beatriz Afonso Menezes & Fabiana Fantinatti-Garboggini

  3. Instituto Biológico, Secretaria de Agricultura e Abastecimento, Centro Experimental do Instituto Biológico Lab. de Bacteriologia Vegetal, Rodovia Heitor Penteado, km 3, Campinas, SP, CEP 13092-543, Brazil

    Mariana Ferreira Tonin, Daniele Bussioli Alves Corrêa & Suzete Aparecida Lanza Destéfano

  4. Embrapa Meio Ambiente, Rodovia SP 340 km 127.5, CP 69, Jaguariúna, SP, CEP 13820-000, Brazil

    Márcia Parma, Itamar Soares de Melo & Tiago Domingues Zucchi

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  1. Claudia Beatriz Afonso Menezes
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  2. Mariana Ferreira Tonin
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  8. Fabiana Fantinatti-Garboggini
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Correspondence to Fabiana Fantinatti-Garboggini.

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Menezes, C.B.A., Tonin, M.F., Corrêa, D.B.A. et al. Chromobacterium amazonense sp. nov. isolated from water samples from the Rio Negro, Amazon, Brazil. Antonie van Leeuwenhoek 107, 1057–1063 (2015). https://doi.org/10.1007/s10482-015-0397-3

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  • DOI: https://doi.org/10.1007/s10482-015-0397-3

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