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Rheinheimera pleomorphica sp. nov., a Novel Alkali-Tolerant Bacteria Isolated from Chilika Lake, India

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Abstract

A novel Gram-negative gamma-proteobacterium, non-sporulating motile, rod or coccus-shaped bacterium designated as strain PKS7T was isolated from a sediment sample collected from Chilika Lake, Odisha, India and characterized taxonomically using a polyphasic approach. The major quinone was Q8 and major cellular fatty acids were C16:0, C17:0, C15:1w8c, C17:1w8c, C12:03-OH. The chemotaxonomic features confirmed the isolate to be a member of genus Rheinheimera. 16SrRNA gene sequence of strain PKS7T was closest in similarity to R. aquimaris SW-353T (99.36% identity), R. muenzenbergensis E49T (98.63%), R. nanhaiensis E407-8T (98.35%), R. japonica KMM 9513T (98.35%) and R. baltica DSM-14885T (98.08%). The 16S rRNA gene sequence-based phylogenetic analysis and sequence similarity between the isolated strain and type strains also revealed its affiliation to genus Rheinheimera. DNA–DNA relatedness with closest type strain R. aquimaris SW-353T was 25.0% (±3.40) and in silico DDH showed values in the range of 17.7–37.1% with the type strains of the genus Rheinheimera for which whole genome sequence are available. Strain PKS7T was also distinguished by a multi-locus sequence analysis (MLST) by alingning gyrB gene sequences of the closest type strains of Rheinheimera. The draft genome of strain PKS7T contained 32 contigs of total size 3,963,569 bp comprising of 3763 predicted coding sequences with a G + C content of 50.7 mol%. Comparision of phenotypic and genotypic data with its closest neighbours and closely related species confirm the strain PKS7T to be recognised as a novel species within the genus Rheinheimera, for which the name Rheinheimera pleomorphica sp. nov. is proposed. The type strain is PKS7T (= KCTC 42365 = JCM 30460).

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Abbreviations

KCTC:

Korean Collection for type culture

DSMZ:

Deutsche Sammlung von Mikroorganismen und Zellkulturen

JCM:

Japan Collection of Microorganisms

FAME:

Fatty acid methyl ester

ZMB/ZMA:

Zobell’s marine broth/agar

L:

Lipid

PL:

Phospholipid

GL:

Glycolipid

AL:

Aminolipid

PE:

Phosphatidylethanolamine

PG:

Phosphatidylglycerol

PN:

Phosphoaminolipid

RAST:

Rapid annotation using subsystem technology

CDSs:

Coding sequences

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Acknowledgements

The authors are grateful to the staffs of Chilika Development Authority (CDA) for their help in collection of samples. We are grateful to Dr. Cathrin Spӧrer and Dr. Susanne Verbarg, DSMZ for their assistance with DNA–DNA hybridization and chemotaxonomic analysis.

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The authors received no specific grant from any funding agency.

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Authors and Affiliations

  1. School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India

    Ananta N. Panda, Lopamudra Ray, Samir Ranjan Mishra & Vishakha Raina

  2. School of Law, KIIT University, Bhubaneswar, Odisha, 751024, India

    Lopamudra Ray

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  1. Ananta N. Panda
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Correspondence to Vishakha Raina.

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The GenBank accession number for 16S rRNA gene sequence of Rheinheimera pleomorphica PKS7T is KJ563231. This whole genome shotgun project has been deposited at DDBJ/ENA/GenBank under the acession SIRR00000000. The version described in this paper is version SIRR01000000. The DPD No is T101043.

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Panda, A.N., Ray, L., Mishra, S.R. et al. Rheinheimera pleomorphica sp. nov., a Novel Alkali-Tolerant Bacteria Isolated from Chilika Lake, India. Curr Microbiol 77, 158–165 (2020). https://doi.org/10.1007/s00284-019-01802-9

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