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Chryseolinea lacunae sp. nov. and Piscinibacter lacus sp. nov. Isolated from Artificial Pond Water

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Abstract

Two Gram stain-negative bacterial strains designated Jin1T and Jin2T were isolated from artificial pond water in the Republic of Korea and investigated in the present study using a polyphasic taxonomic approach. Phylogenetic analysis of the 16S rRNA gene sequences indicated that these two strains belonged to the genera Chryseolinea and Piscinibacter due to their highest similarities with the 16S rRNA gene sequences of Chryseolinea serpens RYGT (98.7%) and Piscinibacter aquaticus IMCC1728T (97.1%), respectively. The dDDH and ANI values were 18.1–20.5% and 68.9–76.8% between whole-genome sequences of strain Jin1T and type trains of the selected taxa, Chryseolinea species, and 20.1–20.2% and 75.5–76.1% between those of strain Jin2T and the type strains of the selected taxa, Piscinibacter species, respectively. A threshold AAI value of 44.7–64.8% for the species boundary (95–96%) was established for strains Jin1T, Jin2T, and type strains of other species involved in the system incidence, which confirms that strains Jin1T and Jin2T represent two new species of the genera Chryseolinea and Piscinibacter, respectively. Based on the phylogenetic, chemotaxonomic, and phenotypic analyses, strains Jin1T and Jin2T represent novel species of the genera Chryseolinea and Piscinibacter, respectively, for which the names Chryseolinea lacunae sp. nov. (type strain Jin1T = KCTC 82562T = NBRC 114837T) and Piscinibacter lacus sp. nov. (type strain Jin2T = KCTC 82556T = NBRC 114838T) have been proposed.

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Acknowledgements

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002203) and by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2022R1F1A1070108).

Funding

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002203) and by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2022R1F1A1070108).

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Authors and Affiliations

  1. Department of Life Science, Dongguk University-Seoul, Goyang, 10326, Republic of Korea

    Inhyup Kim, Geeta Chhetri, Jiyoun Kim, Yoonseop So & Taegun Seo

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  1. Inhyup Kim
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  2. Geeta Chhetri
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Contributions

IK, designed the study, isolated the bacterium, performed the phenotypic and biochemical experiments, analyzed the data, and wrote the original draft; GC, JK, and YS helped with the analysis of taxonomic data; TS designed and supervised the study and edited the original draft; TS designed and supervised the study and edited the original draft.

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Correspondence to Taegun Seo.

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Kim, I., Chhetri, G., Kim, J. et al. Chryseolinea lacunae sp. nov. and Piscinibacter lacus sp. nov. Isolated from Artificial Pond Water. Curr Microbiol 80, 25 (2023). https://doi.org/10.1007/s00284-022-03133-8

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