Abstract
A novel Gram-positive, oval-shaped, non-motile bacterium designated strain 16F1LT was isolated from sediment collected from the Han River in Seoul, Republic of Korea. Based on the 16S rRNA gene sequence (1,448 bp), this strain was identified as a member of the genus Deinococcus that belongs to the class Deinococci. Similarities in the 16S rRNA gene sequence were shown with Deinococcus daejeonensis MJ27T (99.0%), D. grandis DSM 3963T (98.1%), D. radiotolerans C1T (97.5%), and D. caeni Ho-08T (97.2%). Strain 16F1LT was classified as a different genomic species from closely related Deinococcus members, based on less than 70% DNA-DNA relatedness. Genomic DNA G+C content of strain 16F1LT was 67.2 mol%. Strain 16F1LT was found to grow at temperatures of 10–37°C (optimum 25°C) and pH 7–8 (optimum pH 7) on R2A medium, and was catalase-positive and oxidase-negative. Strain 16F1LT showed resistance to gamma radiation (D10 > 2 kGy). In addition, this strain had the following chemotaxonomic characteristics: the major fatty acids were C15:1 ω6c and C16:1 ω7c; the polar lipid profile contained phosphoglycolipids, unknown aminophospholipids, an unknown aminoglycolipid, unknown aminolipids, an unknown glycolipid, an unknown phospholipid, and an unknown polar lipid; the major quinone was MK-8. Phylogenetic, genotypic, phenotypic, and chemotaxonomic characteristics indicated that strain 16F1LT represents a novel species within the genus Deinococcus, for which the name Deinococcus sedimenti sp. nov. is proposed. The type strain is 16F1LT (=KCTC 33796T =JCM 31405T).
Similar content being viewed by others
References
Brooks, B.W. and Murray, R.G.E. 1981. Nomenclature for “Micrococcus radiodurans” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int. J. Syst. Evol. Microbiol. 31, 353–360.
Cappuccino, J.G. and Sherman, N. 2010. Microbiology: a Laboratory Manual, 9th edn, pp. 69–74 & 161–164. Benjamin Cummings, San Francisco, USA.
Cha, S., Srinivasan, S., Seo, T., and Kim, M.K. 2014. Deinococcus radiotolerans sp. nov., a gamma-radiation-resistant bacterium isolated from gamma ray-irradiated soil. Antonie van Leeuwenhoek 105, 229–235.
Ezaki, T., Hashimoto, Y., and Yabuuchi, E. 1989. Fluorometric DNA-DNA hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Evol. Microbiol. 39, 224–229.
Felsenstein, J. 1985. Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool. 20, 406–416.
Frank, J.A., Reich, C.I., Sharma, S., Weisbaum, J.S., Wilson, B.A., and Olsen, G.J. 2008. Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl. Environ. Microbiol. 74, 2461–2470.
Gerhardt, P., Murray, R.G.E., Wood, W.A., and Krieg, N.R. 1994. Methods for General and Molecular Bacteriology. American Society for Microbiology, Washington, DC, USA.
Hiraishi, A., Ueda, Y., Ishihara, J., and Mori, T. 1996. Comparative lipoquinone analysis of influent sewage and activated sludge by high performance liquid chromatography and photodiode array detection. J. Gen. Appl. Microbiol. 42, 457–469.
Im, W.T., Jung, H.M., Ten, L.N., Kim, M.K., Bora, N., Goodfellow, M., Lim, S., Jung, J., and Lee, S.T. 2008. Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 58, 2348–2353.
Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H., et al. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62, 716–721.
Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press, Cambridge, UK.
Lai, W.A., Kämpfer, P., Arun, A.B., Shen, F.T., Huber, B., Rekha, P.D., and Young, C.C. 2006. Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L. Int. J. Syst. Evol. Microbiol. 56, 787–791.
Mattimore, V. and Battista, J.R. 1996. Radioresistance of Deinococcus radiodurans: functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. J. Bacteriol. 178, 633–637.
Mesbah, M., Premachandran, U., and Whitman, W.B. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39, 159–167.
Minnikin, D.E., O’Donnell, A.G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A., and Parlett, J.H. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241.
Oyaizu, H., Stackebrandt, E., Schleifer, K.H., Ludwig, W., Pohla, H., Ito, H., Hirata, A., Oyaizu, Y., and Komagata, K. 1987. A radiation resistant rod-shaped bacterium, Deinobacter grandis gen. nov., sp. nov., with peptidoglycan containing ornithine. Int. J. Syst. Bacteriol. 37, 62–67.
Peng, F., Zhang, L., Luo, X., Dai, J., An, H., Tang, Y., and Fang, C. 2009. Deinococcus xinjiangensis sp. nov., isolated from desert soil. Int. J. Syst. Evol. Microbiol. 59, 709–713.
Rainey, F.A., Nobre, M.F., Schuma, P., Stackebrandt, E., and Da Costa, M.S. 1997. Phylogenetic diversity of the Deinococci as determined by 16S ribosomal DNA sequence comparison. Int. J. Syst. Bacteriol. 47, 510–514.
Rainey, F.A., Ray, K., Ferreira, M., Gatz, B.Z., Nobre, M.F., Bagaley, D., Rash, B.A., Park, M.J., Earl, A.M., Shank, N.C., et al. 2005. Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. Appl. Environ. Microbiol. 71, 5225–5235.
Saitou, N. and Nei, M. 1987. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.
Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI Inc., Newark, DE,USA.
Shashidhar, R. and Bandekar, J.R. 2009. Deinococcus piscis sp. nov., a radiation-resistant bacterium isolated from a marine fish. Int. J. Syst. Evol. Microbiol. 59, 2714–2717.
Srinivasan, S., Kim, M.K., Lim, S., Joe, M., and Lee, M. 2012. Deinococcus daejeonensis sp. nov., isolated from sludge in a sewage disposal plant. Int. J. Syst. Evol. Microbiol. 62, 1265–1270.
Stackebrandt, E. and Goebel, B.M. 1994. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44, 846–849.
Tamaoka, J. and Komagata, K. 1984. Determination of DNA base composition by reversed phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25, 125–128.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725–2729.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24, 4876–4882.
Wayne, L.G., Brenner, D.J., Colwell, R.R., Grimont, P.A.D., Kandler, O., Krichevsky, M.I., Moore, L.H., Moore, W.E.C., Murray, R.G.E., Stackebrandt, E., et al. 1987. International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464.
Weisburg, W.G., Barns, S.M., Pelletier, D.A., and Lane, D.J. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697–703.
Wilson, K. 1997. Preparation of Genomic DNA from Bacteria. Current Protocols in Molecular Biology. 2.4.1-2.4.5. John Wiley and Sons, Inc.
Yoo, S.H., Weon, H.Y., Kim, S.J., Kim, Y.S., Kim, B.Y., and Kwon, S.W. 2010. Deinococcus aerolatus sp. nov. and Deinococcus aerophilus sp. nov., isolated from air samples. Int. J. Syst. Evol. Microbiol. 60, 1191–1195.
Author information
Authors and Affiliations
Corresponding author
Additional information
Supplemental material for this article may be found at http://www.springerlink.com/content/120956.
The NCBI GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 16F1LT is KU758885.
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Lee, JJ., Lee, YH., Park, SJ. et al. Deinococcus sedimenti sp. nov. isolated from river sediment. J Microbiol. 54, 802–808 (2016). https://doi.org/10.1007/s12275-016-6361-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12275-016-6361-8