Production of haploids in barley has been widely used for many years for breeding and breeding method research. Initially the Bulbosum method was available and provided a good random sample of female gametes as plants. Anther culture (male gametes) improved more gradually but has been widely used and more recently, isolated microspore culture has been perfected for barley breeding programmes and research. Thus, barley is now considered the cereal model crop species for haploid production and research. Doubled haploid populations have been extensively utilized in molecular marker work in barley providing detailed chromosome maps. Barley microspores are often selected for biochemical and cytological investigations of androgenesis. With the advancement in molecular, biochemical and cytological tools, haploid research has shifted to sequencing of DNA and transcriptome analyses that reveals hundreds of genes are involved in induction and embryogenesis from microspores. These genes can be identified and associated with various biochemical pathways and the proteins and enzymes involved. Transformation in barley is progressing well using haploid systems
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References
Ayliffe MA, Pallotta M, Langridge P, Pryor AJ (2007) A barley activation tagging system. Plant Mol Biol 64:329–347
Boutilier K, Fiers A, Liu C-M, van der Geest AHM (2005) Biochemical and molecular aspects of haploid embryogenesis. In: Palmer D, Keller W, Kasha KJ (eds) Haploids in crop improvement II. Springer, Heidelberg, pp 73–95
Castillo AM, Vallés MP, Cistué L (2000) Comparison of anther and isolated microspore culture in barley. Effects of culture density and regeneration medium. Euphytica 113:1–8
Caredda S, Devaux P, Sangwan RS, Proult I, Clément C (2004) Plastid ultrastructure and DNA related to albinism in androgenetic embryos of various barley (Hordeum vulgare L.) cultivars. Plant Cell Tiss Org Cult 76:35–43
Carlson AR, Letarte J, Chen J, Kasha KJ (2001) Visual screening of microspore-derived trans-genic barley (Hordeum vulgare L.) with green fluorescent protein (GFP). Plant Cell Rep 20:331–337
Chase SS (1969) Monoploid and monoploid derivatives of maize (Zea mays L.). Bot Rev 35:117–167
Chen X-W, Cistue L, Muñoz-Amatriain M, Sanz M, Romagosa I, Castillo A-M Vallés M-P (2007) Genetic markers for doubled haploid response in barley. Euphytica 158:287–294
Cheng M, Lowe BA, Spencer TM, Ye X, Armstrong CL (2004) Factors influencing Agrobacterium-mediated transformation of monocotyledonous species. In Vitro Cell Dev Biol Plant 40:31–45
Coe EH (1959) A maize line with high haploidy frequency. Amer Nat 93:381–382
Choo TM, Reinbergs E, Kasha KJ (l985) Use of haploids in barley breeding. Plant Breed Rev 3:219–252
Cistué L, Ramos A, Castillo AM, Romagosa I (1994) Production of large numbers of doubled haploid plants from barley anthers pretreated with high concentrations of mannitol. Plant Cell Rep 13:709–712
Cistué L, Vallés MP, Echávarri B, Sanz JM, Castillo A (2003) Barley anther culture. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. A manual. Kluwer, Dordrecht, pp 29–34
Clapham D (1973) Haploid Hordeum plants from anthers in vitro. Z Pflanzenzücht 69:142–155
Clement C, Sangwan RS, Sangwan-Norreel B (2005) Microspore embryo induction and development in higher plants: Cytological and ulrastructural aspects. In: Palmer D, Keller W, Kasha KJ (eds) Haploids in crop improvement II. Springer, Heidelberg, pp 53–72
Close TJ, Wanamaker S, Caldo RA, Turner SM, Ashlock DA, Dickerson JA, Wing RA, Muehlbauer GJ, Kleinhofs A, Wise RP (2004) A new resource for cereal genomics: 22K barley GeneChip comes of age. Plant Physiol 134:960–968
Davies PA (2003) Barley isolated microspore culture (IMC) method In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. A manual. Kluwer, Dordrecht, pp 49–52
Davies PA, Morton S (1998) A comparison of barley isolated microspore and anther culture and the influence of cell culture density. Plant Cell Rep 17:206–210
Devaux P (2003) The Hordeum bulbosum (L.) method. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. A manual. Kluwer, Dordrecht, pp 15–19
Devaux P, Pickering RA (2005) Haploids in the improvement of Poaceae. In: Palmer D, Keller W, Kasha KJ (eds) Haploids in crop improvement II. Springer, Heidelberg, pp 215–242
Devaux P, Zivy M (1994) Protein markers for anther culturability in barley. Theor Appl Genet 88:701–706
Devaux P, Kilian A, Kleinhofs A (1993) Anther culture and Hordeum bulbosum-derived barley doubled haploids: mutations and methylation. Mol Gen Genet 241:674–679
Devaux P, Kilian A, Kleinhofs A (1995) Comparative mapping of the barley genome with male and female recombination-derived, doubled haploid populations. Mol Gen Genet 249:600–608
Devaux P, Zivy M, Kilian A, Kleinhofs A (1996) Doubled haploids in barley. In: Scoles G, Rossnagel B (eds) V International Oat Conference & VII International Barley Genet Symp. Univ. Ext. Press, Saskatchewan, Saskatoon, Canada, pp 213–222
Forster BP, Thomas WTB (2005) Doubled haploids in plant breeding. Plant Breed Rev 25:57–88
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
González-Melendi P, Ramirez C, Testillano PS, Kumlehn J, Risueno MC (2005) Three dimensional confocal and electron microscopy imaging define the dynamics and mechanisms of diploidization at early stages of barley microspore-derived embryogenesis. Planta 222:47–57
Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G, Wenzel G, Herrmann RG (1991) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256
Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura .Nature 204:497
Guha S, Maheshwari SC (1966) Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Nature 212:97–98
Guzy-Wróbelska J, Labocha-Pawlowska A, Kwasniewski M, Szarejko I (2007) Different recombination frequencies in wheat doubled haploid populations obtained through maize pollination and anther culture. Euphytica 156:173–183
Hagberg A, Hagberg G (1980). High frequency of spontaneous doubled haploids in the progeny of an induced mutation in barley. Hereditas 93:341–343
Hayes P, Corey A, DeNoma J (2003) Doubled haploid production in barley using the Hordeum bulbosum (L.) technique. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. A manual. Kluwer, Dordrecht, pp 5–14
Hensel G, Kumlehn J (2004) Genetic transformation of barley (Hordeum vulgare L.) by co-culture of immature embryos with Agrobacterium. In: IS Curtis (ed) Transgenic crops of the world — essential protocols. Kluwer, Dordrecht, pp 35–45
Ho KM, Kasha KJ (1975) Genetic control of chromosome elimination during haploid formation in barley. Genetics 81(2):263–275
Hoekstra S, van Bergen S, van Brouwershaven IR, Schilperoort RA, Wang M (1997) Androgenesis in Hordeum vulgare L: effects of mannitol, calcium and abscisic acid on anther pretreatment. Plant Sci 126:211–218
Hunter CP (1987) Plant generation method. European Patent Application No. 87200773.7
Indrianto A, Barinova I, Touraev A, Heberle-Bors E (2001) Tracking individual wheat micro-spores in vitro: identification of embryogenic microspores and body axis formation in the embryo. Planta 212:163–174
Jacquard C, Wojnarowiez G, Clément C (2003) Anther culture in barley. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. A manual. Kluwer, Dordrecht, pp 21–27
Jacquard C, Asakaviciute R, Hamalian AM, Sangwan RS, Devaux P, Clément C (2006) Barley anther culture: effects of annual cycle and spike position on microspore embryogenesis and albinism. Plant Cell Rep 25:375–381
Jähne A, Lörz H (1995). Cereal microspore culture. Plant Sci 109:1–12
Jähne A, Becker D, Brettschneider R, Lörz H (1994) Regeneration of transgenic, microspore-derived, fertile barley. Theor Appl Genet 89:525–533
Kasha KJ (ed.) (1974a) Haploids in higher plants: advances and potential. Proceedings of the 1st International Symposium, Guelph ON. Univ. Guelph. 421 pp
Kasha KJ (1974b) Haploids from Somatic cells. In: Kasha KJ (ed) Haploids in higher plants: advances and potential. Proceedings of the 1st International Symposium, Guelph ON, Univ. Guelph, pp 67–87
Kasha KJ (2005) Chromosome doubling and recovery of doubled haploid plants. In: Palmer D, Keller W, Kasha K (eds) Haploids in crop improvement II. Springer, Heidelberg, pp 123–152
Kasha KJ (2007) Barley. In: Pua EC, Davey MR (eds) Economic crop biotechnology II. Biotechnology in agriculture and forestry Vo l 59, Chapter 6: 129–150
Kasha KJ, Kao KN (1970) High frequency haploid production in barley (Hordeum vulgare L.) Nature 225:874–876
Kasha KJ, Maluszynski M (2003) Production of doubled haploids in crop plants. An introduction. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. A manual. Kluwer, Dordrecht, pp 1–4Kasha KJ, Simion E (2001) Embryogenesis and plant regeneration from microspores. International Patent Public. No. W0 01/41557. June 14, 2001
Kasha KJ, Simion E, Oro R, Yao QA, Hu TC, Carlson AR (2001a) An improved in vitro technique for isolated microspore culture of barley. Euphytica 120:379–385
Kasha KJ, Hu TC, Oro R, Simion E, Shim YS (2001b) Nuclear fusion leads to chromosome doubling during mannitol pretreatment of barley (Hordeum vulgare L.) microspores. J Exp Bot 52:1227–1238
Kasha KJ, Simion E, Oro R, Shim YS (2003) Barley isolated microspore culture protocol. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. A manual. Kluwer, Dordrecht, pp 43–47
Kleinhofs A, Killian A, Saghai-Maroof MA, Hayes PM, Chen FQ, Lapitan N, Fenwick A, Blake T, Kanazin V, Ananiewe E, Dahleen L, Kudrna D, Bollinger A, Knapp SJ, Liu B, Sorrells M, Heun M, Franckowiak JD, Hoffman D, Skadsen R, Steffenson BJ (1993) A molecular, isozyme and morphological map of the barley (Hordeum vulgare L.) genome. Theor Appl Genet 86:705–712
Koprek T, Rangel S, McElroy D, Louwerse JD, Williams-Carrier E, Lemaux PG (2001) Transposon-mediated single-copy gene delivery leads to increased transgene expression stability in barley. Plant Physiol 125:1354–1362
Kumlehn J, Serazetdinova L, Hensel G, Becker D, Lörz H (2006) Genetic transformation of barley (Hordeum vulgare L.) via infection of androgenetic pollen cultures with Agrobacterium tume-faciens. Plant Biotechnol J 4:251–261
Laurie DA, Bennett MD (1988) The production of haploid wheat plants from wheat x maize crosses. Theor Appl Genet 76:393–397
Lefebvre D, Devaux P (1996) Doubled haploids of wheat from wheat x maize crosses: genotypic influence, fertility and inheritance of the 1BL-1RS chromosome. Theor Appl Genet 93:1267–1273
Letarte J, Simion E, Miner M, Kasha KJ (2006). Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture. Plant Cell Rep 24: 691–698
Li H, Devaux P (2005) Isolated microspore culture over performs anther culture for green plant regeneration in barley (Hordeum vulgare L.). Acta Physiol Plant 27:611–619
Maluszynski M, Kasha KJ, Szarejko I (2003) Published protocols for other crop plant species. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants: A manual, Kluwer, Dordrecht, pp 309–336
Maraschin SF, Caspers M, Potokina E, Wülfert F, Graner A, Spaink HP, Wang M (2005a) cDNA array analysis of stress-induced gene expression in barley androgenesis. Physiol Plantarum 127:535–550
Maraschin SF, de Priestr WS, Spaink HP, Wang M (2005b). Androgenic switch: An example of plant embryogenesis from the male gametophyte perspective. J Exp Bot 56:1711–1726
Meng L, Ziv M, Lemaux PG (2006) Nature of stress and transgene locus influences transgene expression stability in barley. Plant Mol Biol 62:15–28
Morgensen HL (1982) Double fertilization in barley and the cytological explanation for haploid embryo formation, embryoless caryopses, and ovule abortion. Carlsberg Res Comm 47:313–354
Mordhorst AP, Lörz H (1993) Embryogenesis and development of isolated barley (Hordeum vulgare L.) microspores are influenced by the amount and composition of nitrogen sources in culture media. J Plant Physiol 142:485–492
Mujeeb-Kazi A, Riera-Lizarazu O (1996) Polyhaploid production in the Triticeae by sexual hybridization. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, Vol 1. Kluwer, Dordrecht, pp 275–296
Muñoz-Amatriain M, Svensson JT, Castillo A-M, Cistue L, Close TJ (2006) Transcriptome analysis of barley anthers: effect of mannitol treatment on microspore embryogenesis Physio Plantarum 127:551–560
Paire A, Devaux P, Lafitte C, Dumas C, Matthys-Rochon E (2003) Proteins produced by barley microspores and their derived androgenic structures promote in vitro zygotic maize embryo formation. Plant Cell Tiss Org Cult 73:167–176
Pickering RA (1983) The location of a gene for incompatibility between Hordeum vulgare L. and Hordeum bulbosum L. Heredity 51:455–459
Pickering RA (1985) Partial control of chromosome elimination by temperature in immature embryos of Hordeum vulgare L. x H. bulbosum L. Euphytica 34:869–874
Pickering RA, Devaux P (1992) Haploid production: approaches and use in plant breeding. In: Shewry PR (ed) Barley: genetics, molecular biology and biotechnology. CAB International, Wallingford, pp 511–539
Pickering RA, Rennie WF (1990) The evaluation of superior Hordeum bulbosum L. genotypes for use in a doubled haploid barley breeding programme. Euphytica 45:251–255
Reynolds TL (2000) Effects of calcium on embryogenic induction and the accumulation of absci-sic acid, and an early cysteine-labeled methallothionein gene in androgenic microspores of Triticum aestivum. Plant Sci 150:201–207
Roberts-Oehlschlager SL, Dunwell JM (1990) Barley anther culture: pre-treatment on mannitol stimulates production of microspore-derived embryos. Plant Cell Tiss Org Cult 20:235–240
San Noeum LH (1976) Haploïdes d'Hordeum vulgare L par culture in vitro d'ovaires non fecondés. Ann Amélior Plant 26:751–783
Saski C, Lee S-B, Fjelljeim S, Guda C, Jansen RK, Luo H, Tomkins J, Rognli OA, Daniell H, Clarke JL (2007). Complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolour and Agrostis stolonifera, and comparative analyses with other grass genomes. Theor Appl Genet 115:571–590
Schön C, Sanchez M, Blake T, Hayes PM (1990) Segregation of Mendelian markers in doubled haploid and F2 progeny of a barley cross. Hereditas 113:69–72
Shariatpanahi ME, Bal U, Heberle-Bors E, Touraev A (2006). Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiol Plantarum 127:519–534
Shim YS, Kasha KJ (2003) The influence of pretreatment on cell stage progression and the time of DNA synthesis in barley (Hordeum vulgare L.) uninucleate microspores. Plant Cell Rep 21:1065–1071
Shim YS (2006) Improvement of Microspore Transformation in Barley (Hordeum vulgare L). Ph D Thesis University of Guelph, pp170
Snape JW, Sitch LA, Simpson E, Parker BB (1988) Tests for the presence of gametoclonal variation in barley and wheat doubled haploids produced using the Hordeum bulbosum system. Theor Appl Genet 75:509–513
Simpson E, Snape JW, Finch RA (1980) Variation between Hordeum bulbosum genotypes in their ability to produce haploids of barley, Hordeum vulgare. Z Pflanzenzüchtg 85:205–211
Subrahmanyam NC, Kasha KJ (1975) Chromosome doubling of barley haploids by nitrous oxide and colchicine treatments. Can J Genet Cytol 17:573–583
Sunderland N (1974) Anther culture as a means of haploid induction. In: Kasha KJ (ed) Haploids in higher plants — advances and potential, Univ of Guelph, pp 91–122
Szarejko I (2003) Anther culture for double haploid production in barley (Hordeum vulgare L) In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants: A manual. Kluwer, Dordrecht, pp 35–42
Szarejko I, Kasha KJ (1991) Induction of anther culture derived doubled haploids in barley. Cereal Res Commun 19:219–237
Thomas WTB, Forster BP, Gertsson B (2003) Doubled haploids in breeding. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants: A manual. Kluwer, Dordrecht, pp 337–350
Tingay S, McElroy D, Kalla R, Fieg S, Wang M, Thornton S, Brettell R (1997) Agrobacterium tumefaciens-mediated barley transformation. Plant J 11:1369–1376
Travella S, Ross SM, Harden J, Everett C, Snape JW, Harwood WA (2005) A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques. Plant Cell Rep 23:780–789
Varshney RK, Sigmund R, Borner A, Korzun V, Stein N, Sorrells ME, Langridge P, Graner A (2005) Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice. Plant Sci 168:195–202
Varshney RK, Marcel TC, Ramsay L, Russell J, Roder MS, Stein N, Waugh R, Langridge P, Niks RE, Graner A (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theor Appl Genet 114:1091–1103
von Wettstein D (2004) Transgenic barley. In: Spunar J, Janikova J (eds) Proceedings of the 9th International Barley Genetics Symposium, Brno CZ, pp 144–150
Vrinten PL, Nakamura T, Kasha KJ (1999) Characterization of cDNAs expressed in the early stages of microspore embryogenesis in barley (Hordeum vulgare L.). Plant Mol Biol 41(4):455–463
Wang M, Hoekstra S, van Bergen S, Lamers GEM, Oppedijk BJ, van der Heijden MW, de Priester W, Schilperoort RA (1999) Apoptosis in developing anthers and the role of ABA in this process during androgenesis in Hordeum vulgare L. Plant Mol Biol 39:489–501
Yao QA, Simion E, William M, Krochko J, Kasha KJ (1997) Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.) Genome 40:570–581
Yean-San LH (1987) Gynogenèse in vitro. Variabilité des haploides doublés issus d'androgenèse in vitro et de croisement interspécifique chez Hordeum vulgare L. Thèse Doctorat d'Etat, Université de Paris-sud, France
Zhang H, Sreenivasulu N, Weschke W, Stein N, Rudd S, Radchuk V, Potkina E, Scholz U, Schweizer P, Zierold U, Langridge P, Varshney RK, Wobus U, Graner A (2004) Large-scale analysis of the barley transcriptome based on expressed sequence tags. Plant J 40: 276–290
Ziauddin A, Marsolais A, Simion E, Kasha KJ (1992) Improved plant regeneration from wheat anther and barley microspore culture using phenylaceticacid (PAA). Plant Cell Rep 11:489–498
Zivy M, Devaux P, Blaisonneau J, Jean R, Thiellement H (1992) Segregation distortion and linkage studies in microspore derived doubled haploid lines of Hordeum vulgare L. Theor Appl Genet 83:919–924
Zoriniants S, Tashpulatov AS, Heberle-Bors E, Touraev A (2005) The role of stress in the induction of haploid microspore embryogenesis. In: Palmer D, Keller W, Kasha K (eds) Haploids in crop improvement II. Springer, Heidelberg, pp 35–52
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Devaux, P., Kasha, K.J. (2009). Overview of Barley Doubled Haploid Production. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_3
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