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Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture

  • Cell Biology and Morphogenesis
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An Erratum to this article was published on 04 July 2006

Abstract

The objective of this study was to improve induction of embryogenesis in wheat microspore culture in order to obtain a high number of regenerable embryos. The arabinogalactan (AG) Larcoll and the arabinogalactan-protein (AGP) from gum arabic were tested on two spring genotypes to see if they could increase microspore viability and induce embryogenesis in the microspore culture. Adding Larcoll significantly decreased microspore mortality in both genotypes regardless of the presence or absence of ovaries in the culture. Similarly, gum arabic had a strong effect on the number of embryos produced and regenerated green plants. In fact, by using only gum arabic we were able to obtain green plants from wheat microspore cultures without the presence of ovaries. In addition to preventing a high mortality rate of the cells, our results show that the induction of embryogenesis in wheat microspore cultures is strongly affected by the use of both AG or AGP.

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Abbreviations

AGs :

Arabinogalactan

AGPs :

Arabinogalactan-protein

DHs :

Doubled haploid

ELSs :

Embryo-like-structures

PCDs :

Programmed cell death

References

  • Akiyama Y, Kato K (1981) An extracellular arabinogalactan-protein from Nicotiana tabacum. Phytochemistry 20:2507–2510

    Article  CAS  Google Scholar 

  • Anderson RL, Clarke AE, Jermyn MA, Knox RB, Stone BA (1977) A carbohydrate-binding arabinogalactan-protein from liquid suspension cultures of endosperm from Lolium multiflorum. Aust J Plant Physiol 4:143–158

    Article  CAS  Google Scholar 

  • Bajaj YPS (1990) In vitro production of haploids and their use in cell genetics and plant breeding. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Haploids in crop improvement, vol 12. Springer, Berlin Heidelberg New York, pp 1–44

    Google Scholar 

  • Baldwin TC, McCann MC, Roberts K (1993) A novel hydroxyproline-deficient arabinogalactan protein secreted by suspension-cultured cells of Daucus carota. Plant Physiol 103:115–123

    PubMed  CAS  Google Scholar 

  • Borderies G, le Béchec M, Rossignol M, Laflitte C, Deunnff E, Beckert M, Dumas C, Matthys-Rochon E (2004) Characterization of proteins secreted during maize microspore culture: arabinogalactan proteins (AGPs) stimulate embryo development. Eur J Cell Biol 83:205–212

    Article  PubMed  CAS  Google Scholar 

  • Bruins MBM, Rakoczy-Trojanowska M, Snijders CHA (1996) Isolated microspore culture in wheat (Triticum aestivum L): the effect of co-culture of wheat or barley ovaries on embryogenesis. Cereal Res Commun 24:401–408

    Google Scholar 

  • Carlson AR, Letarte J, Chen J, Kasha KJ (2001) Visual screening of microspore-derived transgenic barley (Hordeum vulgare L.) with green-fluorescent protein. Plant Cell Rep 20:331–337

    Article  CAS  Google Scholar 

  • Chaves I, Regalado AP, Chen M, Ricardo CP, Showalter AM (2002) Programmed cell death induced by (β-d-galactosyl)3 Yariv reagent in Nicotiana tabacum BY-2 suspension-cultured cells. Physiol Plant 116:548–553

    Article  CAS  Google Scholar 

  • Clarke AE, Anderson RL, Stone BA (1979) Form and function of arabinogalactans and arabinogalactan-proteins. Phytochemistry 18:521–540

    Article  CAS  Google Scholar 

  • Comeau A, Nadeau P, Plourde A, Simard R, Maës O, Kelly S, Harper L, Lettre J, Landry B, St-Pierre C-A (1992) Media for the in ovulo culture of proembryos of wheat and wheat-derived interspecific hybrids or haploids. Plant Sci 81:117–125

    Article  CAS  Google Scholar 

  • Egersdotter U, Von Arnold S (1995) Importance of arabinogalactan-proteins for the development of somatic embryos of Norway spruce (Picea abies). Physiol Plant 93:334–345

    Article  Google Scholar 

  • Folling L, Olesen A (2001) Transformation of wheat (Triticum aestivum L.) microspore-derived callus and microspores by particle bombardment. Plant Cell Rep 20:629–636

    Article  CAS  Google Scholar 

  • Gao M, Showalter AM (1999) Yariv reagent treatment induces programmed cell death in Arabidopsis cell cultures and implicates arabinogalactan protein involvement. Plant J 19:321–331

    Article  PubMed  CAS  Google Scholar 

  • Harwood WA, Bean SJ, Chen D-F, Mullineaux PM, Snape JW (1995) Transformation studies in Hordeum vulgare using a highly regenerable microspore system. Euphytica 85:113–118

    Article  Google Scholar 

  • Hoggart RM, Clarke AE (1984) Arabinogalactans are common components of angiosperm styles. Phytochemistry 8:1571–1573

    Article  Google Scholar 

  • Hu T, Kasha KJ (1997) Improvement of isolated microspore culture of wheat (Triticum aestivum L.) through ovary co-culture. Plant Cell Rep 16:520–525

    Article  CAS  Google Scholar 

  • Hu TC, Ziauddin A, Simion E, Kasha KJ (1995) Isolated microspore culture of wheat (Triticum aestivum L.) in a defined media. I. Effects of pretreatment, isolation methods, and hormones. In Vitro Cell Dev Biol 31:79–83

    Article  CAS  Google Scholar 

  • Ingram HM, Power JB, Lowe KC, Davey MR (1999) Optimization of procedures for microprojectile bombardment of microspore-derived embryos in wheat. Plant Cell Tissue Organ Cult 57:207–210

    Article  CAS  Google Scholar 

  • Jähne A, Lörz H (1995) Cereal microspore culture. Plant Sci 109:1–12

    Article  Google Scholar 

  • Kasha KJ, Maluszynski M (2003) Production of doubled haploids in crop plants (Introduction). In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants, a manual. Kluwer Academic, Dordrecht Boston London, pp 1–4

    Google Scholar 

  • Kasha KJ, Simion E (2001) Embryogenesis and plant regeneration from microspores. International Patent Application No. PCT/CAOO/01436. USA patent #6,821,028 issued Nov. 2, 2004

  • Kasha KJ, Simion E, Miner M, Letarte J, Hu TC (2003a) Haploid wheat isolated microspore culture protocol. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants, a manual. Kluwer Academic, Dordrecht Boston London, pp 77–81

    Google Scholar 

  • Kasha KJ, Simion E, Oro R, Shim YS (2003b) Barley isolated microspore culture protocol. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants, a manual. Kluwer Academic, Dordrecht Boston London, pp 43–48

    Google Scholar 

  • Kreuger M, van Holst GJ (1993) Arabinogalactan proteins are essential in somatic embryogenesis of Daucus carota L. Planta 189:243–248

    Article  CAS  Google Scholar 

  • Kreuger M, van Holst G-J (1995) Arabinogalactan protein epitopes in somatic embryogenesis of Daucus carota L. Planta 197:135–141

    Article  CAS  Google Scholar 

  • Kreuger M, Postma E, Brouwer V, van Holst G-J (1995) Somatic embryogenesis of Cyclamen persicum in liquid medium. Physiol Plant 94:605–612

    Article  CAS  Google Scholar 

  • Kunz C, Islam SMS, Berberat J, Peter SO, Büter B, Stamp P, Schmid JE (2000) Assessment and improvement of wheat microspore derived embryo induction regeneration. J Plant Physiol 156:190–196

    CAS  Google Scholar 

  • Majewska-Sawka A, Nothnagel EA (2000) The multiple role of arabinogalactan-proteins in plant development. Plant Physiol 122:3–9

    Article  PubMed  CAS  Google Scholar 

  • McCabe PF, Leaver CJ (2000) Programmed cell death in cell cultures. Plant Mol Biol 44:359–368

    Article  PubMed  CAS  Google Scholar 

  • McCabe PF, Valentine TA, Forsberg LS, Pennell RI (1997) Soluble signals from cells identified at the cell wall, establish a developmental pathway in carrot. Plant Cell 9:2225–2241

    Article  PubMed  CAS  Google Scholar 

  • Mentewab A, Letellier V, Marque C, Sarrafi A (1999) Use of anthocyanin biosynthesis stimulatory genes as markers for the genetic transformation of haploid embryos and isolated microspores in wheat. Cereal Res Commun 27:17–24

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nothnagel EA (1997) Proteoglycans and related components in plant cells. Int Rev Cytol 174:195–291

    Article  PubMed  CAS  Google Scholar 

  • Osman ME, Williams PA, Menzies AR, Phillips GO (1993) Characterization of commercial samples of GA. J Agric Food Chem 41:71–77

    Article  CAS  Google Scholar 

  • Puolimatka M, Pauk J (1999) Impact of explant type, duration, and initiation time on the co-culture effect in isolated microspore culture of wheat (Triticum aestivum L.). J Plant Physiol 154:367–373

    Google Scholar 

  • Raghavan V (1986) Embryogenesis in angiosperms: a developmental and experimental study. Cambridge University Press, New York, 303 pp

    Google Scholar 

  • Redha A, Islam SMS, Büter B, Stamp P, Schmid JE (2000) The improvement in regenerated doubled haploids from anther culture of wheat by anther transfer. Plant Cell Tissue Organ Cult 63:167–172

    Article  CAS  Google Scholar 

  • Showalter AM (2001) Arabinogalactan-proteins: structure, expression, and function. Cell Mol Life Sci 58:1399–1417

    Article  PubMed  CAS  Google Scholar 

  • Toonen MAJ, Schmidt EDL, van Kammen A, de Vries SC (1997) Promotive and inhibitory effects of diverse arabinogalactan-proteins on Daucus carota L. somatic embryogenesis. Planta 203:188–195

    CAS  Google Scholar 

  • Touraev A, Vicente O, Herberle-Bors E (1997) Initiation of microspore embryogenesis by stress. Trends Plant Sci 2:285–303

    Article  Google Scholar 

  • Vrinten PL, Nakamura T, Kasha KJ (1999) Characterization of cDNAs expressed in the early stages of microspore embryogenesis in barley Hordeum vulgare L. Plant Mol Biol 41:455–463

    Article  PubMed  CAS  Google Scholar 

  • Wheatley WG, Marsolais AA, Kasha KJ (1986) Microspore growth and anther staging in wheat anther culture. Plant Cell Rep 5:47–49

    Article  Google Scholar 

  • Yao QA, Simion E, William M, Krochko J, Kasha KJ (1997) Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.). Genome 40:570–581

    Article  PubMed  Google Scholar 

  • Yariv J, Rapport MM, Graf L (1962) The interaction of glycosides and saccharides with antibody to the corresponding phenylazo glycoside. Biochem J 85:383–388

    PubMed  CAS  Google Scholar 

  • Yariv J, Lis H, Katchalski E (1967) Precipitation of arabic acid and some seed polysaccharides by glycosylphenylazo dyes. Biochem J 105:1c–2c

    PubMed  CAS  Google Scholar 

  • Zheng MY, Liu W, Weng Y, Polle E, Konzak CF (2001) Culture of freshly isolated wheat (Triticum aestivum L.) microspores treated with inducer chemicals. Plant Cell Rep 20:685–690

    Article  CAS  Google Scholar 

  • Zheng MY, Weng Y, Liu W, Konzak CF (2002) The effect of ovary-conditioned medium on microspore embryogenesis in common wheat (Triticum aestivum L.). Plant Cell Rep 20:802–807

    Article  CAS  Google Scholar 

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Acknowledgements

The authors gratefully acknowledge funding provided by NSERC, Monsanto and the Ontario Ministry of Agriculture and Food

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Authors and Affiliations

  1. Department of Plant Agriculture, University of Guelph, Ontario, Canada, N1G 2W1

    Jocelyne Letarte, Ecaterina Simion, Mai Miner & Ken J. Kasha

Authors
  1. Jocelyne Letarte
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  2. Ecaterina Simion
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  3. Mai Miner
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  4. Ken J. Kasha
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Corresponding author

Correspondence to Ken J. Kasha.

Additional information

Communicated by M. C. Jordan

An erratum to this article can be found at http://dx.doi.org/10.1007/s00299-006-0180-z

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Letarte, J., Simion, E., Miner, M. et al. Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture. Plant Cell Rep 24, 691–698 (2006). https://doi.org/10.1007/s00299-005-0013-5

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  • DOI: https://doi.org/10.1007/s00299-005-0013-5

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