Abstract
Male (anther culture) and female (Hordeum bulbosum) derived, doubled haploid populations were used to map the barley genome and thus determine the different recombination rates occurring during meiosis in the F1 hybrid donor plants. The anther culture-derived (male recombination) population showed an 18% overall increase in recombination rate. This increased recombination rate was observed for every chromosome and most of the chromosome arms. Examination of linkage distances between individual markers revealed eight segments with significantly higher recombination in the anther culture-derived population, and one in the Hordeum bulbosum-derived population. Very strong distortions of single locus segregations were observed in the anther culture-derived population, but map distances were not affected significantly by these distortions. There were 1.047 and 0.912 recombinations per chromosome in the anther culture and Hordeum bulbosum-derived doubled haploid populations, respectively.
Similar content being viewed by others
References
Bojko M (1983) Human meiosis. VIII. Chromosome pairing and formation of the synaptonemal complex in oocytes. Carlsberg Res Commun 48:457–483
Busso CS, Liu CJ, Hash CT, Witcombe JR, Devos KM, deWet JMJ, Gale MD (1995) Analysis of recombination rate in female and male gemetogenesis in pearl millet (Pennisetum glaucum) using RFLP markers. Theor Appl Genet 90:242–246
Chen CC, Kasha KJ, Marsolais A (1984) Segmentation patterns and mechanisms of genome multiplication in cultured microspores of barley. Can J Genet Cytol 26:475–483
Chen F, Hayes PM (1989) A comparison of Hordeum bulbosum-mediated haploid production efficiency in barley using in vitro floret and tiller culture. Theor Appl Genet 77:701–704
Devaux P (1992) Haploidy in barley and wheat improvement. In: Datto Y, Dumas C, Gallais A (eds) Reproductive biology and plant breeding. Springer-Verlag, Berlin, pp 139–151
Devaux P, Zivy M (1994) Protein markers for anther culturability in barley. Theor Appl Genet 88:701–706
Devaux P, Hou L, Ullrich SE, Huang Z, Kleinhofs A (1993) Factors affecting anther culturability of recalcitrant barley genotypes. Plant Cell Rep 13:32–36
De Vicente MC, Tanksley SD (1991) Genome-wide reduction in recombination of backcross progeny derived from male versus female gametes in an interspecific cross of tomato. Theor Appl Genet 83:173–178
Donis-Keller H, Green P, Helms C, Cartinhour S, Weiffenbach B, Stephens K, Keith TP, Bowden DW, Smith DR, Lander E, Botstein D, Akots G, Rediker KS, Gravius T, Brown VA, Rising MB, Parker C, Powers JA, Watt DE, Kauffman ER, Bricker A, Phipps P, Muller Kahle H, Fulton TR, Ng S, Schumm JW, Braman JC, Knowlton RG, Barker DF, Crooks SM, Lincoln SE, Daly MJ, Abrahamson J (1987) A genetic linkage map of the human genome. Cell 51:319–337
Foroughi-Wehr B, Friedt W, Wenzel G (1982) Field experiments with anther-derived lines of barley (Hordeum vulgare) and rye (Secale cereale). In: Giles KL, Sen SK (eds) Plant cell culture in crop improvement. Plenum Publishing, New York
Franzone PM, Fishbeck G, Foroughi-Wehr B, Friedt W (1984) Analysis of origin of androgenetic plants of Hordeum vulgare L. by the use of balanced lethal systems. Z. Pflanzenzüchtg 93:320–330
Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G, Wenzel G, Herrmann RG (1991) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256
Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map of barley (Hordeum vulgare). Genome 34:437–447
Jensen J (1981) Effect of temperature on genetic recombination in barley. Hereditas 94:215–218
Julier C, Nakamura Y, Lathrop M, O'Connell P, Leppert M, Litt M, Mohandas T, Lalouel J-M, White R (1990a) A detailed genetic map of the long arm of chromosome 11. Genomics 7:335–345
Julier C, Nakamura Y, Lathrop M, O'Connell P, Leppert M, Mohandas T, Lalouel J-M, White R (1990b) A primary map of 24 loci on human chromosome 16. Genomics 6:419–427
Kasha KJ, Reinbergs E (1982) Recent developments in the production and utilization of haploids in barley. In: Asher MJC, Ellis RP, Whitehouse RNH (eds) Proceedings 4th International Barley Genetics Symposium, July 22–29, 1981. Edinburgh University Press, Edinburgh, pp 655–665
Kleinhofs A (1994) Barley Steptoe × Morex map. File available via Internet Gopher, host greengenes.cit.cornell.edu, port 70, menu “Grains files to browse”/“Barley Steptoe × Morex map”
Kleinhofs A, Kilian A, Kudrna D, Bollinger J, Hayes P, Chen FQ, Saghai Maroof MA, Lapitan N, Fenwick A, Blake TK, Kanazin V, Ananiev E, Dahleen L, Liu B, Sorrells M, Heun M, Franckowiak JD, Hoffman D, Skadsen R, Steffenson BJ, Knapp SJ (1993) A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86:705–712
Lagercrantz U, Lydiate D (1995) RFLP mapping in Brassica nigra indicates different recombination rates in male and female meioses. Genome 38:255–264
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Liu BH, Knapp SJ (1990) GMENDEL: a program for Mendelian segregation and linkage analysis of individual or multiple progeny populations using log-likelihood ratios. J Hered 81: 407
Maeda T (1939) Chiasma studies in the silk worm, Bombyx mori L. Jpn J Genet 15:118–127
Moore G, Abbo S, Cheung W, Foote T, Gale MD, Koebner R, Leitch A, Leitch I, Money T, Stancombe P, Yano M, Flavell R (1993) Key features of cereal genome organization as revealed by the use of cytosine-methylation sensitive restriction endonucleases. Genomics 15:472–482
Morgan TH (1914) No crossing over in the male of Drosophila of genes in the second and third pair of chromosomes. Biol Bull (Woods Hole) 26:195–204
Nakamura Y, Lathrop M, O'Connell P, Leppert M, Barker D, Wright E, Skolnick M, Kandoleon S, Litt M, Lalouel J-M, White R (1989) A mapped set of DNA markers for human chromosome 17. Genomics 2:302–309
Nilsson N-O, Pelger S (1991) The relationship between natural variation in chiasma frequencies and recombination frequencies in barley. Hereditas 115:121–126
Nilsson N-O, Sall T, Bengtsson BO (1993) Chiasma and recombination data in plants: are they compatible? Trends Gene 9:344–348
O'Connell P, Lathrop M, Law M, Leppert M, Nakamura Y, Hoff M, Kumlin E, Thomas W, Elsner T, Ballard L, Goodman P, Azen E, Sadler J, Cai G, Laloul J-M, White R (1987) A primary genetic linkage map for human chromosome 12. Genomics 1:89–102
Pickering RA, Devaux P (1992) Haploid production: approaches and use in plant breeding. In: Shewry PR (ed) Barley: genetics, molecular biology and biotechnology. CAB Press, Wallingford, pp 511–539
Powell JB, Nilan RA (1963) Influence of temperature on crossing over in an inversion heterozygote in barley. Crop Sci 3:11–13
Powell W, Borrino EM, Allison MJ, Griffiths DW, Asher MJC, Dunwell JM (1986) Genetical analysis of microspore-derived plants of barley (Hordeum vulgate). Theor Appl Genet 72:619–626
Reeves RH, Crowley MR, O'hara BF, Gearhart JD (1990) Sex, strain, and species differences affect recombination across an evolutionarily conserved segment of mouse chromosome 16. Genomics 8:141–148
Robertson DS (1984) Different frequency in the recovery of crossover products from male and female gametes of plants hypoploid for B-A translocations in maize. Genetics 107:117–134
Rouyer F, De La Chapelle A, Andersson M, Weissenbach J (1990) An interspersed repeated sequence specific for human subtelomeric regions. EMBO J 9:505–514
Saccone S, De Sarino A, Della Valle G, Bernardi G (1992) The highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes. Proc Natl Acad Sci USA 89:4913–4917
Sall T, Flink J, Bengtsson BO (1990) Genetic control of recombination in barley. Hereditas 112:157–170
Shiroishi T, Sagai T, Hanzawa N, Gotoh H, Moriwaki K (1991) Genetic control of sex-dependent meiotic recombination in the major histocompatibility complex of the mouse. EMBO J 10:681–686
Sinha RP, Helgason SB (1969) The action of actinomycin D and diepoxybutane on recombination of two closely linked loci in Hordeum. Can J Genet Cytol 11:745–751
Sokoloff A (1964) Sex and crossing over in Tribolium castaneum. Genetics 50:491–496
Sved JA, Blackman LM, Gilchrist AS, Engels WR (1991) High levels of recombination induced by homologous P elements in Drosophila melanogaster. Mol Gen Genet 225:443–447
Thompson DM, Chalmers K, Waugh R, Foster BP, Thomas WTB, Caligari PDS, Powell W (1991) The inheritance of genetic markers in microspore-derived plants of barley Hordeum vulgare L. Theor Appl Genet 81:487–492
Watson ID, Callan HG (1963) The form of bivalent chromosomes in newt oocytes at first metaphase of meiosis. Quart J Microsc Sci 104:281–295
Weiffenbach B, Falls K, Bricker A, Hall L, McMahon J, Wasmuth J, Funanage V, Donis-Keller H (1991) A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10:173–185
Weitkamp LR, Guttormsen SA, Costello Leary P (1982) Equine gene mapping: a sex difference in recombination frequency for linkage group II. Animal Blood Groups Biochem Genet 13:305–306
Zetka MC, Rose AM (1990) Sex-related differences in crossing over in Caenorhabditis elegans. Genetics 126:355–363
Zivy M, Devaux P, Blaisonneau J, Jean R, Thiellement H (1992) Segregation distortion and linkage studies in microspore-derived double haploid lines of Hordeum vulgare L. Theor Appl Genet 83:919–924
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Devaux, P., Kilian, A. & Kleinhofs, A. Comparative mapping of the barley genome with male and female recombination-derived, doubled haploid populations. Molec. Gen. Genet. 249, 600–608 (1995). https://doi.org/10.1007/BF00418029
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00418029