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Chromosome Doubling and Recovery of Doubled Haploid Plants

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Haploids in Crop Improvement II

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 56))

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References

  • Afza R, Xie J, Shen M, Zapata-Arias FJ, Fundi HK, Lee K-S, Bobadilla-Mucino E, Kodym A (2001) Detection of androclonal variation in anther-cultured rice lines using RAPDs. In Vitro Cell Dev Biol Plant 37:644–647

    CAS  Google Scholar 

  • Antoine-Michard S, Beckert M (1997) Spontaneous versus colchicine-induced chromosome doubling in maize anther culture. Plant Cell Tissue Organ Cult 48:203–207

    Article  Google Scholar 

  • Arzani A, Darvey NL (2001) The effect of colchicine on triticale anther-derived plants: microspore pretreatment and haploid plant treatment using a hydroponic recovery system. Euphytica 122:235–241

    Article  CAS  Google Scholar 

  • Barclay IR (1975) High frequencies of haploid production in wheat (Triticum aestivum L.) by chromosome elimination. Nature 256:410–411

    Article  Google Scholar 

  • Barnabas B, Obert B, Kovacs G (1999) Colchicine, an efficient genome doubling agent for maize (Zea mays L.) microspores cultured in anthers. Plant Cell Rep 18:858–862

    CAS  Google Scholar 

  • Binarova P, Hause G, Cenklova V, Cordewener JHG, Van Lookeren Campagne MM (1997) A short severe heat shock is required to induce embryogenesis in late bicellular pollen of Brassica napus L. Sex Plant Reprod 10:200–208

    Article  Google Scholar 

  • Bingham E, Goose R, Woodfield D, Kidwell K (1994) Complementary gene interactions in alfalfa are greater in autotetraploids than in diploids. Crop Sci 34:823–829

    Article  Google Scholar 

  • Bishonoi U, Jain R, Rohilla J, Chowdhury V, Gupta K, Chowdhury J (2000) Anther culture of recalcitrant indica × basmati rice hybrids. Euphytica 114:93–101

    Google Scholar 

  • Bordes J, Dumas de Vaulx R, Lapierre A, Pollacsek M (1997) Haplodiploidization of maize (Zea mays L.) through induced gynogenesis assisted by glossy markers and its use in breeding. Agronomie 17:291–297

    Google Scholar 

  • Bouvier L, Guerif P, Djulbic M, Durel D, Chevreau E, Lespinasse Y (2002) Chromosome doubling of pear haploid plants and homozygosity assessment using isozyme and microsatellite markers. Euphytica 123:255–262

    Article  CAS  Google Scholar 

  • Chase SS (1949) The reproductive success of monoploid maize. Am J Bot 36:795–796

    Google Scholar 

  • Chase SS (1974) Utilization of haploids in plant breeding: breeding diploid species. In: Kasha KJ (ed) Haploids in higher plants, advances and potential. University of Guelph, Guelph, pp 211–230

    Google Scholar 

  • Chen CC, Howarth MJ, Peterson RL, Kasha KJ (1984) Ultrastructure of androgenic microspores of barley during the early stages of anther cultures. Can J Genet Cytol 26:484–491

    Google Scholar 

  • Chen JL, Beversdorf WD (1992) Production of spontaneous diploid lines from isolated microspores following cryopreservation in spring rapeseed (Brassica napus L). Plant Breed 108:324–327

    Google Scholar 

  • Coe EH Jr (1959) A line of maize with high haploid frequency. Am Nat 93:381–382

    Google Scholar 

  • Cordewener JHG, Custers JBM, Van Lookeran Campagne MM (1998) Microspore culture. A model for investigating the role of stress in the induction of embryogenesis. In: Chupeau Y, Caboche M, Henry Y (eds) Androgenesis and haploid plants. INRA edn. Springer, Berlin Heidelberg New York, pp 54–68

    Google Scholar 

  • De Fossard RA (1974) Terminology in ‘haploid’ work. In: Kasha KJ (ed) Haploids in higher plants: advances and potential. University of Guelph, Guelph, pp 403–410

    Google Scholar 

  • Devaux P, Kilian A, Kleinhofs A (1995) Comparative mapping of the barley genome with male and female-derived doubled haploid populations. Mol Gen Genet 249:600–608

    Article  PubMed  CAS  Google Scholar 

  • Dewitte W, Murray JAH (2003) The plant cell cycle. Annu Rev Plant Biol 54:235–264

    Article  PubMed  CAS  Google Scholar 

  • Douches DS, Mass DL (1998) Comparison of FDR and SDR-derived tetraploid progeny from using haploids of Solanum tuberosum L. that produce mixed modes of 2n eggs. Theor Appl Genet 97:1307–1313

    Article  Google Scholar 

  • Eady C, Lindsay K, Twell D (1995) The significance of microspore division and division symmetry for vegetative cell-specific transcription and generative cell differentiation. Plant Cell 7:65–74

    Article  PubMed  CAS  Google Scholar 

  • Eder J, Chalyk S (2002) In vitro haploid induction in maize. Theor Appl Genet 104:703–708

    Article  PubMed  CAS  Google Scholar 

  • Engvild KC, Linde-Laursen I, Lundqvist A (1972) Anther cultures of Datura innoxia: floral bud stage and embryoid level of ploidy. Hereditas 72:331–332

    Google Scholar 

  • Fan Z, Armstrong K, Keller W (1988) Development of microspores in vivo and in vitro in Brassica napus. Protoplasma 147:191–199

    Article  Google Scholar 

  • Foisset N, Delourme R, Lucas MO, Renard M (1997) In vitro androgenesis and segregation distortion in Brassica napus L: spontaneous versus colchicine-doubled lines. Plant Cell Rep 16:464–468

    Article  CAS  Google Scholar 

  • Geoffriau E, Kahne R, Bellamy C, Rancillac M (1997) Ploidy stability and in vitro chromosome doubling in gynogenic clones of onion (Allium cepa L.). Plant Sci 122:201–208

    Article  CAS  Google Scholar 

  • Guerel S, Guerel E, Kaya Z (2000) Doubled haploid plant production from unpollinated ovules of sugar beet (Beta vulgaris L.). Plant Cell Rep 19:1155–1159

    Google Scholar 

  • Guo YD, Pulli S (2000a) An efficient androgenic embryogenesis and plant regeneration method through isolated microspore culture in timothy (Phleum pratense L.). Plant Cell Rep 19:761–767

    CAS  Google Scholar 

  • Guo YD, Pulli S (2000b) Isolated microspore culture and plant regeneration in rye (Secale cereale L). Plant Cell Rep 19:875–880

    CAS  Google Scholar 

  • Guha S, Maheshwari SC (1967) Development of embryoids from pollen grains of Datura in vitro. Phytomorphology 17:454–461

    Google Scholar 

  • Hansen NJP, Andersen SB (1996) In vitro chromosome doubling potential of colchicine, oryzalin, trifluralin and APM in Brassica napus microspore culture. Euphytica 88:159–164

    Article  CAS  Google Scholar 

  • Hansen NJP, Andersen SB (1998a) Efficient production of doubled haploid wheat plants by in vitro treatment of microspores with trifluralin or APM. Plant Breed 117:401–405

    CAS  Google Scholar 

  • Hansen NJP, Andersen SB (1998b) In vitro chromosome doubling with colchicine during microspore culture in wheat (Triticum aestivum L.). Euphytica 102:101–108

    Article  CAS  Google Scholar 

  • Hansen AL, Gertz A, Joersbo M, Andersen SB (2000) Chromosome doubling in vitro with amiprophos-methyl in Beta vulgaris ovule culture. Acta Agri Scand 50:89–95

    CAS  Google Scholar 

  • Heberle-Bors E (1989) Isolated pollen culture in tobacco: plant reproductive development in a nutshell. Sex Plant Reprod 2:1–10

    Article  Google Scholar 

  • Heberle-Bors E (1998) Experimental control of pollen development. In: Chupeau Y, Caboche M, Henry Y (eds) Androgenesis and haploid plants. INRA edn. Springer, Berlin Heidelberg New York, pp 38–53

    Google Scholar 

  • Herrera JC, Moreno LG, Acuna JR, De Pena M, Osorio D (2002) Colchicine-induced microspore embryogenesis in coffee. Plant Cell Tissue Organ Cult 71:89–92

    Article  CAS  Google Scholar 

  • Hoekstra S, van Bergen S, van Brouwershaven IR, Schilperoot RA, Wang M (1997) Androgenesis in Hordeum vulgare L.: effects of mannitol, calcium and abscisic acid on anther pretreatment. Plant Sci 126:211–218

    Article  CAS  Google Scholar 

  • Horlow C, Raquin C (1998) A critical analysis of existing haploidization techniques. In: Chupeau Y, Caboche M, Henry Y (eds) Androgenesis and haploid plants. INRA edn. Springer, Berlin Heidelberg New York, pp 7–23

    Google Scholar 

  • Hu H (1983) Genetic stability and variability of pollen-derived plants. In: Sen SK, Giles KL (eds) Plant cell culture in crop improvement. Basic life science, vol 22. Plenum Press, New York, pp 145–157

    Google Scholar 

  • Hu H (1996) Chromosome engineering in the Triticeae using pollen-derived plants (CETPP). In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 2. Kluwer, Dordrecht, pp 203–223

    Google Scholar 

  • Hu T, Kasha KJ (1997) Improved embryogenesis from isolated microspore culture of wheat (Triticum aestivum L.) through ovary co-culture. Plant Cell Rep 16:520–525

    Article  CAS  Google Scholar 

  • Hu TC, Kasha KJ (1999) A cytological study of pretreatments used to improve isolated microspore cultures of wheat (Triticum aestivum L.) cv. Chris. Genome 42:432–441

    Article  Google Scholar 

  • Ilic-Grubor K, Attree SM, Fowke LC (1998) Induction of microspore-derived embryos of Brassica napus L with polyethylene glycol (PEG) as osmoticum in a low sucrose medium. Plant Cell Rep 17:329–333

    CAS  Google Scholar 

  • Indrianto A, Barinova I, Touraev A, Heberle-Bors E (2001) Tracking individual wheat microspores in vitro: identification of embryogenic microspores and body axis formation in the embryo. Planta 212:163–174

    Article  PubMed  CAS  Google Scholar 

  • Jain SM, Sopory SK, Veilleux RE (eds) (1996/1997) In vitro haploid production in higher plants, vol 1–5. Kluwer, Dordrecht

    Google Scholar 

  • Jensen CJ (1974) Chromosome doubling techniques in haploids. In: Kasha KJ (ed) Haploids in higher plants, advances and potential. University of Guelph, Guelph, pp 153–190

    Google Scholar 

  • Kao KN, Michayluk MR (1974) A method for high frequency intergeneric fusion of plant protoplasts. Planta 115:355–367

    Article  CAS  Google Scholar 

  • Kasha KJ (2003) Plants from haploid cells. In: Saxena PK (ed) Single cells to plants: concepts and applications. Oxford and IBH Publ Co Pvt Ltd, New Delhi

    Google Scholar 

  • Kasha KJ, Kao KN (1970) High frequency haploid production in barley (Hordeum vulgare L.). Nature 225:874–876

    Article  PubMed  CAS  Google Scholar 

  • Kasha KJ, Hu TC, Oro R, Simion E, Shim YS (2001) Nuclear fusion leads to chromosome doubling during mannitol pretreatment of barley (Hordeum vulgare L.) microspores. J Exp Bot 52:1227–1238

    Article  PubMed  CAS  Google Scholar 

  • Kato A (2002) Chromosome doubling of haploid maize seedlings using nitrous oxide gas at the flower primordial stage. Plant Breed 121:370–377

    Google Scholar 

  • Keller WA, Melchers G (1973) The effect of high pH and calcium on tobacco leaf protoplast fusion. Z Naturforsch 28c:737–741

    Google Scholar 

  • Kermicle JL (1969) Androgenesis conditioned by a mutation in maize. Science 166:1422–1424

    CAS  PubMed  Google Scholar 

  • Kiss J, Kondrak M, Toerjek O, Kiss E, Gyulai G, Mazik-Toekei K, Heszky L (2001) Morphological and RAPD analysis of poplar trees of anther culture origin. Euphytica 188:213–221

    Google Scholar 

  • Kiviharja E, Puolimatka M, Saastamoinen M, Pehu E (2000) Extension of anther culture to several genotypes of cultivated oats. Plant Cell Rep 19:674–679

    Google Scholar 

  • Laurie DA, Bennett MD (1986) Wheat by maize hybridization. Can J Genet Cytol 28:313–316

    Google Scholar 

  • Li H, Devaux P (2003) High frequency regeneration of barley doubled haploid plants from isolated microspore culture. Plant Sci 164:379–386

    CAS  Google Scholar 

  • Lim KB, Ramanna MS, de Jong JH, Jacobsen E, van Tuyl JM (2001) Indeterminate meiotic restitution (IMR): a novel type of meiotic nuclear restitution mechanism detected in interspecific lily hybrids by GISH. Theor Appl Genet 103:219–230

    Article  CAS  Google Scholar 

  • Lionneton E, Beuret W, Delaitre C, Ochatt S, Rancillac M (2001) Improved microspore culture and doubled-haploid plant regeneration in the brown condiment mustard (Brassica juncea). Plant Cell Rep 20:126–130

    Article  CAS  Google Scholar 

  • Liu W, Zheng MY, Konzak CF (2002) Improving green plant production via isolated microspore culture in bread wheat (Triticum aestivum L.). Plant Cell Rep 20:821–824

    CAS  Google Scholar 

  • Logue SJ (1996) Genetic stability in microspore-derived doubled haploids. In: Jain SM, Sopory SK, Veilleux R (eds) In vitro haploid production in higher plants, vol2. Kluwer, Dordrecht, pp 1–51

    Google Scholar 

  • Lough RC, Varrieur JM, Veilleux RE (2001) Selection inherent in monoploid derivation mechanisms for potato. Theor Appl Genet 103:178–184

    Article  CAS  Google Scholar 

  • Magnard J-L, Le Deunff E, Domenech J, Rogowsky PM, Testillano PS, Rougier M, Risueño MC, Vergne P, Dumas C (2000) Genes normally expressed in the endosperm are expressed at early stages of microspore embryogenesis in maize. Plant Mol Biol 44:559–574

    Article  PubMed  CAS  Google Scholar 

  • Martin B, Widholm JM (1996) Ploidy of small individual embryo-like structures from maize anther cultures treated with chromosome doubling agents and calli derived from them. Plant Cell Rep 15:781–785

    CAS  Google Scholar 

  • McCormick S (1993) Male gametophyte development. Plant Cell 5:1265–1275

    Article  PubMed  Google Scholar 

  • Mentewab A, Sarrafi A (1997) Androgenic ability and chromosome doubling by different colchicine treatments in anther culture of hexaploid wheat genotypes (Triticum aestivum L.). Cereal Res Commun 25:897–903

    CAS  Google Scholar 

  • Mochida K, Tsujimoto H (2001) Production of wheat haploids by pollination with Job’s tears (Coix lachryma-jobi L.). J Hered 92:81–83

    Article  PubMed  CAS  Google Scholar 

  • Mok D, Peloquin SJ (1975) Three mechanisms of 2n pollen formation in diploid potatoes. Can J Genet Cytol 17:217–225

    Google Scholar 

  • Muranty H, Sourdille P, Bernard S, Bernard M (2002) Genetic characterization of spontaneous diploid androgenetic wheat and triticale plants. Plant Breed 121:470–474

    CAS  Google Scholar 

  • Nitsch C (1974) Pollen culture — a new technique for mass production of haploid and homozygous plants. In: Kasha KJ (ed) Haploids in higher plants, advances and potential. University of Guelph, Guelph, pp 123–135

    Google Scholar 

  • Pechan PM, Keller WA (1988) Identification of potentially embryogenic microspores in Brassica napus. Physiol Plant 74:377–384

    Google Scholar 

  • Pechan PM, Smykal P (2001) Androgenesis: affecting the fate of the male gametophyte. Physiol Plant 111:1–8

    Article  CAS  Google Scholar 

  • Peloquin S, Yerk G, Werner J, Darmo E (1989) Potato breeding with haploids and 2n gametes. Genome 31:1000–1004

    Google Scholar 

  • Raghavan V (1976) Role of the generative cell in androgenesis in henbane. Science 191:338–339

    Google Scholar 

  • Rao PS, Suprasanna P (1996) Methods to double haploid chromosome numbers. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol1. Kluwer, Dordrecht, pp317–339

    Google Scholar 

  • Redha A, Islam SMS, Büter B, Stamp P, Schmid JE (2000) The improvement in regenerated doubled haploids from anther culture of wheat by anther transfer. Plant Cell Tissue Organ Cult 63:167–172

    Article  CAS  Google Scholar 

  • Reynolds TL (1997) Pollen embryogenesis. Plant Mol Biol 33:1–10

    Article  PubMed  CAS  Google Scholar 

  • Rose MD (1996) Nuclear fusion in the yeast Saccharomyces cerevisiae. Annu Rev Cell Dev Biol 12:663–695

    Article  PubMed  CAS  Google Scholar 

  • Saidi N, Chlyah O, Chlyah H (1998) Production of green haploid durum wheat plants by pollination of wheat by maize. Can J Bot 76:652–656

    Google Scholar 

  • Saisingtong S, Schmid JE, Stamp P, Büter B (1996) Colchicine-mediated chromosome doubling during anther culture of maize (Zea mays L.). Theor Appl Genet 92:1017–1023

    CAS  Google Scholar 

  • Sharma H, Yang Y, Ohm H (2002) An assessment of doubled haploid production in soft red winter wheat by wheat × corn wide crosses. Cereal Res Commun 30:269–275

    Google Scholar 

  • Shim YS, Kasha KJ (2003) The influence of pretreatment on cell stage progression and the time of DNA synthesis in barley (Hordeum vulgare L.) uninucleate microspores. Plant Cell Rep 21:1065–1071

    Article  PubMed  CAS  Google Scholar 

  • Sibi ML, Kobaissi A, Shekafandeh A (2001) Green haploid plants from unpollinated ovary culture in tetraploid wheat (Triticum durum Defs.). Euphytica 122:351–359

    Article  Google Scholar 

  • Simmonds DH, Keller WA (1999) Significance of preprophase bands of microtubules in the induction of microspore embryogenesis of Brassica napus. Planta 208:383–391

    Article  CAS  Google Scholar 

  • Stober A, Hess D (1997) Spike pretreatments, anther culture conditions, and anther culture response of 17 German varieties of spring wheat (Triticum aestivum L.). Plant Breed 116:443–447

    Google Scholar 

  • Subrahmanyam NC, Kasha KJ (1975) Chromosome doubling of barley haploids by nitrous oxide and colchicine treatments. Can J Genet Cytol 17:573–583

    CAS  Google Scholar 

  • Sun C-S (1978) Androgenesis of cereal crops. In: Proc Symp on Plant Tissue Culture. Science Press, Peking, pp 117–124

    Google Scholar 

  • Sunderland N (1974) Anther culture as a means of haploid production. In: Kasha KJ (ed) Haploids in higher plants, advances and potential. University of Guelph, Guelph, pp 91–122

    Google Scholar 

  • Sunderland N, Evans LJ (1980) Multicellular pollen formation in cultured barley anthers. J Exp Bot 31:501–514

    Google Scholar 

  • Sunderland N, Collins GB, Dunwell JM (1974) The role of nuclear fusion in pollen embryogenesis of Datura innoxia Mill. Planta (Berl) 117:227–241

    Article  Google Scholar 

  • Testillano PS, Ramirez C, Domenech J, Coronado MJ, Vergne P, Matthys Rochon E, Risueno MC (2002) Young microspore-derived maize embryos show two domains with defined features also present in zygotic embryogenesis. Int J Dev Biol 46:1035–1047

    PubMed  CAS  Google Scholar 

  • Thiebaut J, Kasha KJ (1978) Modification of the colchicine technique for chromosome doubling of barley haploids. Can J Genet Cytol 20:513–521

    CAS  Google Scholar 

  • Thiebaut J, Kasha KJ, Tsai A (1979) Influence of plant development stage, temperature and plant hormones on chromosome doubling of barley haploids using colchicine. Can J Bot 57:480–483

    CAS  Google Scholar 

  • Thomas WTB, Forster BP, Gertsson B (2003) Doubled haploids in breeding. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants, a manual. Kluwer, Dordrecht, pp 337–350

    Google Scholar 

  • Tosca A, Pandolfi R, Citterio S, Fasoli A, Sgorbati S (1996) Determination by flow cytometry of the chromosome doubling capacity of colchicine and oryzalin in gynogenetic haploids of Gerbera. Plant Cell Rep 14:455–458

    Google Scholar 

  • Touraev A, Pfosser M, Vincente O, Heberle-Bors E (1996) Stress as the major signal controlling the developmental fate of tobacco microspores: towards a unified model of induction of microspore/pollen embryogenesis. Planta 200:144–152

    Article  CAS  Google Scholar 

  • Touraev A, Vincente O, Heberle-Bors E (1997) Initiation of microspore embryogenesis by stress. Trends Plant Sci 2:297–302

    Article  Google Scholar 

  • Touraev A, Pfosser M, Heberle-Bors E (2001) The microspore: a haploid multipurpose cell. Adv Bot Res 35:53–109

    Google Scholar 

  • Turcotte EL, Feaster CV (1974) Semigametic production of cotton haploids. In: Kasha KJ (ed) Haploids in higher plants, advances and potential. University of Guelph, Guelph, pp 53–64

    Google Scholar 

  • Twell D, Howden R (1998) Mechanisms of asymmetric division and cell fate determination in developing pollen. In: Chupeau Y, Caboche M, Henry Y (eds) Androgenesis and haploid plants. INRA edn. Springer, Berlin Heidelberg New York, pp 69–103

    Google Scholar 

  • Twell D, Park SK, Lalanne E (1998) Asymmetric division and cell-fate determination in developing pollen. Trends Plant Sci 3:305–310

    Article  Google Scholar 

  • Vantard M, Cowling R, Delichère C (2000) Cell cycle regulation of the microtubular cytoskeleton. Plant Mol Biol 43:691–703

    Article  PubMed  CAS  Google Scholar 

  • Veilleux R (1985) Diploid and polyploid gametes in crop plants: mechanisms of formation and utilization in plant breeding. Plant Breed Rev 3:253–288

    Google Scholar 

  • Wedzony M, Marcinska I, Pontika A, Slusarkiewicz-Jarzina A, Wozna J (1998) Production of doubled haploids in triticale (× Triticosecale Wittin.) by means of crosses with maize (Zea mays L.) using picloram and dicamba. Plant Breed 117:211–215

    CAS  Google Scholar 

  • Wheatley WG, Marsolais AA, Kasha KJ (1986) Microspore growth and anther staging in barley anther culture. Plant Cell Rep 5:47–49

    Article  Google Scholar 

  • Wilson HM, Mix G, Foroughi-Wehr B (1978) Early microspore divisions and subsequent formation of microspore calluses at high frequency in anthers of Hordeum vulgare L. J Exp Bot 108:227–238

    Google Scholar 

  • XuHan X, Jing H-C, Cheng X-F, Iwanowska A, Kieft H, Bergervost JHW, Groot SPC, Bino RJ, van Lammeren AMM (1999) Polyploidization in embryogenic microspore cultures of Brassica napus L cv. Topas enables the generation of doubled haploid clones by somatic embryogenesis. Protoplasma 208:240–247

    Article  Google Scholar 

  • Zaki MAM, Dickenson HG (1991) Microspore-derived embryos in Brassica: the influence of division symmetry in pollen mitosis 1 to embryogenic development. Sex Plant Reprod 44:48–55

    Google Scholar 

  • Zamani I, Kovács G, Gouli-Vavdinoudi E, Roupakias DG, Barnabás B ( (2000) Regeneration o fertile doubled haploid plants from colchicine-supplemented media in wheat anther culture. Plant Breed 119:461–465

    Article  CAS  Google Scholar 

  • Zhao J-P, Simmonds DH, Newcombe W (1996) High frequency production of doubled haploid plants of Brassica napus cv. Topas derived from colchicine-induced microspore embryogenesis without heat shock. Plant Cell Rep 15:668–671

    CAS  Google Scholar 

  • Zhou WJ, Tang GX, Hagberg P (2002a) Efficient production of doubled haploid plants by immediate colchicine treatment of isolated microspores in winter Brassica napus. Plant Growth Regul 37:185–192

    Article  CAS  Google Scholar 

  • Zhou WJ, Hagberg P, Tang GX (2002b) Increasing embryogenesis and doubling efficiency by immediate colchicine treatment of isolated microspores in spring Brassica napus. Euphytica 128:27–34

    Article  CAS  Google Scholar 

  • Ziauddin A, Kasha KJ (1990) Genetic stability in haploid cell cultures. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 12. Haploids in crop improvement I. Springer, Berlin Heidelberg New York, pp 83–89

    Google Scholar 

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Kasha, K.J. (2005). Chromosome Doubling and Recovery of Doubled Haploid Plants. In: Don Palmer, C., Keller, W.A., Kasha, K.J. (eds) Haploids in Crop Improvement II. Biotechnology in Agriculture and Forestry, vol 56. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-26889-8_7

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