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Sequence analysis and expression profiles of TaABI5, a pre-harvest sprouting resistance gene in wheat

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Abstract

Pre-harvest sprouting (PHS) is the germination of grains in the spike under wet conditions before harvest, which causes a severe reduction in crop yield and flour quality. The phytohormone abscisic acid plays a key role in regulating seed dormancy, which inhibits pre-harvest spouting or vivipary for many important crops. TaABI5, belonging to a family of basic leucine zipper transcription factors could regulate a subset of late embryogenesis abundant genes during seed development, was isolated and characterized. Phylogenetic analysis revealed that TaABI5 homologous gene belonged to the ABI5 subgroup of the ABF/AREB/ABI5 subfamily. TaABI5 consist of four conserved domains and one bZip domain. The expression pattern of TaABI5 indicated that it was seed-specific and accumulated at late stages of seed development. Accumulation of TaABI5 in synthetic hexaploid wheat SHW-L1 (PHS resistance) was 8.93-fold higher than that in wheat cultivar Chuanmai 32 (PHS susceptible) at 25 days post anthesis. Four expression quantitative trait loci (eQTL) of TaABI5 on chromosome 2DS, 4DS, 6DS, and 7DL were characterized from SHW-L1/Chuanmai 32 derived recombinant inbred line population.

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Acknowledgements

This work was supported by the National Basic Research Program of China (2014CB147200), the National Natural Science Foundation of China (31171555 and 31571654), the Research Fund for the Doctoral Program of Higher Education in China (20135103110008), and the Program for Scientific Research Team of Universities in Sichuan (KYTD201406).

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Authors and Affiliations

  1. College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, China

    Ke Zhou

  2. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, China

    Ke Zhou, Jian Yang, Zao-Xia Wang & Ji-Rui Wang

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  1. Ke Zhou
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  2. Jian Yang
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  3. Zao-Xia Wang
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  4. Ji-Rui Wang
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Correspondence to Ji-Rui Wang.

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Ke Zhou, Jian Yang, Zao-Xia Wang, and Ji-Rui Wang, declares that they have no conflict of interest.

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Ke Zhou and Jian Yang are Co-first authors.

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Zhou, K., Yang, J., Wang, ZX. et al. Sequence analysis and expression profiles of TaABI5, a pre-harvest sprouting resistance gene in wheat. Genes Genom 39, 161–171 (2017). https://doi.org/10.1007/s13258-016-0483-6

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