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The wheat PKABA1-interacting factor TaABF1 mediates both abscisic acid-suppressed and abscisic acid-induced gene expression in bombarded aleurone cells

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Abstract

To investigate the crosstalk of abscisic acid (ABA) and gibberellin (GA) signaling in wheat (Triticum aestivum), we have focused on the transcription factor TaABF1. TaABF1 (a member of the ABA response element binding factor family) physically interacts with PKABA1, a signaling component in the ABA-suppression of GA-induced gene expression in cereal grains. Constitutive expression of TaABF1 in aleurone cells of imbibing grains completely eliminated GA-induced expression from the Amy32b promoter. In addition to its effect on Amy32b, TaABF1 strongly stimulated expression from the ABA-inducible HVA1 and HVA22 promoters. Overexpression of TaABF1 fully substituted for exogenous ABA in the induction of these two promoters. The introduction of a construct directing RNA inhibition (RNAi) of TaABF1 did not prevent either ABA-mediated or PKABA1-mediated suppression of Amy32b expression. Similarly, the RNAi construct did not prevent ABA-induction of HVA1. These results suggest that another protein may act redundantly with TaABF1 during cereal imbibition. Although TaABF1 mRNA was downregulated during imbibition of afterripened grains, transcript levels were not significantly altered by exogenous GA or ABA, suggesting that upregulation of TaABF1 at the mRNA level is not required for its role in ABA signaling. We propose a model in which TaABF1 is involved in two separate branches of ABA signaling. In this model, TaABF1 acts downstream of PKABA1 in ABA-suppression of GA-induced gene expression, and participates (independently of PKABA1) in the stimulation of ABA-induced genes.

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Abbreviations

ABA:

Abscisic acid

ABF:

Abscisic acid response element binding factor

ABRE:

Abscisic acid response element

ABRC:

Abscisic acid response complex

GA:

Gibberellin

nt:

Nucleotide

qRT-PCR:

Quantitative (real time) reverse transcription-polymerase chain reaction

RNAi:

RNA inhibition

SnRK2:

Snf1-Related kinase 2

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Acknowledgements

The authors gratefully acknowledge Xialou Zou and Angi Zhang for their help with particle bombardment. They also thank Peter Quail for providing plasmid pAHC17, Nam-Hai Chua for providing plasmid pSKint, and Camille Steber for providing Brevor wheat grains. This work was supported in part by a grant from the US National Science Foundation (IOB-0443676), by a US National Institutes of Health Grant (P20-RR-016463) from the INBRE program of the National Center for Research Resources, and by a Colby College Natural Sciences Division Research Grant. We also thank the Wattis Dumke Foundation for funding the purchase of a real time thermal cycler to facilitate qRT-PCR experiments.

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  1. Department of Biology, Colby College, 5723 Mayflower Hill, Waterville, ME, 04901, USA

    Russell R. Johnson

  2. Department of Biology, University of Nevada, 4505 Maryland Parkway, Las Vegas, NV, 89154, USA

    Margaret Shin & Jeff Q. Shen

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  1. Russell R. Johnson
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  2. Margaret Shin
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Correspondence to Russell R. Johnson.

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Johnson, R.R., Shin, M. & Shen, J.Q. The wheat PKABA1-interacting factor TaABF1 mediates both abscisic acid-suppressed and abscisic acid-induced gene expression in bombarded aleurone cells. Plant Mol Biol 68, 93–103 (2008). https://doi.org/10.1007/s11103-008-9354-0

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