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Mapping diploid wheat homologues of Arabidopsis seed ABA signaling genes and QTLs for seed dormancy

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Abstract

Abscisic acid (ABA) sensitivity in embryos is one of the key factors in the seed dormancy of wheat. Many ABA signaling genes have been isolated in Arabidopsis, while only a few wheat homologues have been identified. In the present study, diploid wheat homologues to Arabidopsis ABA signaling genes were identified by in silico analysis, and mapped them using a population of diploid wheat recombinant inbred lines derived from a cross between Triticum monococcum (Tm) and T. boeoticum (Tb). Four diploid wheat homologues, TmVP1, TmABF, TmABI8 and TmERA1 were located on chromosome 3Am and TmERA3 was on the centromere region of chromosome 5Am. In two consecutive year trials, one major QTL on the long arm of 5Am, two minor QTLs on the long arm of 3Am and one minor QTL on the long arm of 4Am were detected. The 5Am QTL explained 20–27% of the phenotypic variations and the other three QTLs each accounted for approximately 10% of the phenotypic variations. Map positions of the loci of TmABF and TmABI8 matched the LOD peaks of the two QTLs on 3Am, indicating that these two homologues are possible candidate genes for seed dormancy QTLs. Moreover, we have found two SNPs result in amino acid substitutions in TmABF between Tb and Tm. Comparison of the marker positions of QTLs for seed dormancy of barley revealed that the largest QTL on 5Am may be orthologous to the barley seed dormancy QTL, SD1, whereas there seems no orthologous QTL to the corresponding barley SD2 locus.

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Acknowledgments

We thank Professor T. Sasakuma at the Kihara Institute of Yokohama City University and Professor K. Noda, Okayama University for providing us with diploid wheat RILs for mapping, Dr. M. Sameri at the National Institute of Agrobiological Sciences, and Dr. Y. Turuspekov at Montana State University for their kind advice, Dr. S. Utsugi at Okayama University and Professor R.R. Finkelstein at University of California, Santa Barbara for critical reading of the manuscript, and H. Morishige, K. Kanamaru and H. Miki for their technical assistance. This research was supported by a Brand Nippon grant from the Ministry of Agriculture, Forestry and Fisheries of Japan.

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Authors and Affiliations

  1. Wheat and Barley Biotechnology Research Team, National Institute of Crop Science, Tsukuba, 305-8518, Ibaraki, Japan

    Shingo Nakamura

  2. Plant Genome Research Unit, National Institute of Agrobiological Sciences, Tsukuba, 305-8602, Ibaraki, Japan

    Takao Komatsuda

  3. Department of Crop Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080-8555, Hokkaido, Japan

    Hideho Miura

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  1. Shingo Nakamura
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  2. Takao Komatsuda
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Correspondence to Shingo Nakamura.

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Communicated by M. Sorrells.

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Nakamura, S., Komatsuda, T. & Miura, H. Mapping diploid wheat homologues of Arabidopsis seed ABA signaling genes and QTLs for seed dormancy. Theor Appl Genet 114, 1129–1139 (2007). https://doi.org/10.1007/s00122-007-0502-8

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