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GmFtsH9 expression correlates with in vivo photosystem II function: chlorophyll a fluorescence transient analysis and eQTL mapping in soybean

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Abstract

Filamentation temperature-sensitive H (FtsH) is an ATP-dependent zinc metalloprotease involved in diverse biological functions. There are 12 FtsH proteins in Arabidopsis, among which AtFtsH2 plays an important role in regulating the turnover of photosystem II (PSII) reaction center D1 protein and the development of the photosynthetic apparatus. Here, we have identified 11 FtsH genes in the soybean genome by a bioinformatics approach. These soybean FtsH genes corresponded to seven Arabidopsis FtsH genes, suggesting that the main characteristics of soybean FtsH genes were formed before the evolutionary split of soybean and Arabidopsis. Phylogenetic analyses allowed us to clone a soybean AtFtsH2-like gene designated as GmFtsH9. The predicted protein of GmFtsH9 consists of 690 amino acids and contains three typical FtsH proteins conserved domains. The expression level of GmFtsH9 was determined in a soybean recombinant inbred line population under a pot experiment conducted for measuring chlorophyll a fluorescence transient parameters, photosynthetic CO2 fixation rate (P N), and seed yield. Expression quantitative trait loci (eQTL) mapping revealed two trans-acting eQTLs for GmFtsH9. The significant correlation of gene expression level with chlorophyll a fluorescence transient parameters and the presence of overlapping eQTL (QTL) between gene expression level and chlorophyll a fluorescence transient parameters indicated that GmFtsH9 could be involved in regulating PSII function. These results further lead to the understanding of the mechanism underlying FtsH gene expression, and contribute to the development of marker-assisted selection breeding programs for modulating soybean FtsH gene expression.

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Abbreviations

ABS/RC:

Light energy absorbed by per RC

eQTL:

Expression quantitative trait locus

ETo/TRo (ψEo):

Probability that a trapped exciton moves an electron into the electron transport chain beyond Q −A

Fv/Fm:

Ratio of variable fluorescence to maximum fluorescence in the dark-adapted state, related to maximum quantum yield of PSII primary photochemistry

Fv′/Fm′:

Ratio of variable fluorescence to maximum fluorescence in the light-adapted state, related to the maximum quantum yield of PSII primary photochemistry

JIP-test:

A procedure for quantification of prompt fluorescence transients

PIABS :

Performance index on absorption basis, PIABS = (RC/ABS)[φPo/(1 − φPo)][ψEo/(1 − ψEo)]

P N :

Photosynthetic CO2 fixation rate

ΦPSII:

Actual quantum yield of PSII primary photochemistry in the light-adapted state

qN:

Non-photochemical quenching coefficient

qP:

Photochemical quenching coefficient

QTL:

Quantitative trait locus

RC:

Reaction center of PSII

REo/ETo (δRo):

Probability that an electron beyond Q −A reduces photosystem I acceptors

RIL:

Recombinant inbred line

TRo/ABS (φPo):

Flux ratio of trapping per absorption, φPo ≡ 1 – F 0/F M = F V/F M

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Acknowledgments

This work was supported in part by the National Key Basic Research Program of China (973 Program) (2010CB125906, 2009CB118400), the National Natural Science Foundation of China (31000718, 30800692). We thank Dr. Han Zhao from the Jiangsu Academy of Agricultural Science for assistance in bioinformatics analysis of soybean FtsH genes, and the two anonymous reviewers for their valuable comments and discussions.

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Authors and Affiliations

  1. National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Zhitong Yin, Fanfan Meng, Haina Song, Xiaolin Wang, Maoni Chao, Guozheng Zhang & Deyue Yu

  2. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225009, China

    Zhitong Yin & Dexiang Deng

  3. Photosynthesis Research Laboratory, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Xiaoming Xu

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  1. Zhitong Yin
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  9. Deyue Yu
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Correspondence to Deyue Yu.

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Z. Yin and F. Meng contributed equally to this work.

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Yin, Z., Meng, F., Song, H. et al. GmFtsH9 expression correlates with in vivo photosystem II function: chlorophyll a fluorescence transient analysis and eQTL mapping in soybean. Planta 234, 815–827 (2011). https://doi.org/10.1007/s00425-011-1445-5

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