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Genetic diversity, population structure and association analysis in linseed (Linum usitatissimum L.)

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Abstract

The present investigation aimed to explore the level of genetic diversity, determine the population structure in a larger set of germplasm of linseed using microsatellite marker and identify linked markers through association mapping. A total of 168 accessions of linseed were evaluated for major agro-economic traits and SSRs markers deployed for diversity assessment. A total of 337 alleles were amplified by 50 SSRs ranging from 2 to 13 with an average of 6.74 ± 2.8 alleles per loci. The neighbor joining based clustering grouped all the accessions into three major clusters that were also confirmed by scatter plot of PCoA. While model based clustering determined four sub-populations (K = 4). Further, analysis of molecular variance analysis considering three population showed that maximum variation (79%) was within the population. We identified one putative SSR marker (Lu_3043) linked with days to 50% flowering through both GLM and MLM analysis of association mapping. The results of this preliminary study revealed genetic diversity, population structure in linseed and linked marker which could be utilized in future breeding program.

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Acknowledgements

Authors thank the Director, CSIR-NBRI, Lucknow for providing the facilities to carry out the present investigation. Financial support in form of DST-INSPIRE Fellowship to Chandrawati is gratefully acknowledged.

Author’s contribution

HKY, SAR, RK and SK conceived and designed the research. C, NS, carried out the experiments. PKS provided genetic materials. HKY, VKY, SAR and RK analyzed data. C, NS, SK and HKY wrote the manuscript. All authors read and approved the manuscript.

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Authors and Affiliations

  1. CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, UP, 226001, India

    Chandrawati, Neha Singh, V. K. Yadav, S. A. Ranade & Hemant Kumar Yadav

  2. Academy of Scientific and Innovative Research, New Delhi, India

    Chandrawati, V. K. Yadav & Hemant Kumar Yadav

  3. Uttar Pradesh Council of Agricultural Research (UPCAR), Vibhuti Khand, Gomatinagar, Lucknow, 226010, India

    Rajendra Kumar & Sujit Kumar

  4. AICRP on Linseed, CSAUA&T, Kanpur, India

    P. K. Singh

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  1. Chandrawati
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  2. Neha Singh
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  3. Rajendra Kumar
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  4. Sujit Kumar
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  5. P. K. Singh
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  6. V. K. Yadav
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  7. S. A. Ranade
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  8. Hemant Kumar Yadav
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Correspondence to Hemant Kumar Yadav.

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Chandrawati, Singh, N., Kumar, R. et al. Genetic diversity, population structure and association analysis in linseed (Linum usitatissimum L.). Physiol Mol Biol Plants 23, 207–219 (2017). https://doi.org/10.1007/s12298-016-0408-5

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