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Development and characterization of polymorphic microsatellite markers in Linum usitatissimum

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Abstract

Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum.

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Acknowledgments

This work was funded by National Special Funds for Public Benefit Research of China (No. nyhyzx07-018-03) and Modern Agricultural Industry R&D System in China (No nycytx-19-E14).

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Authors and Affiliations

  1. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, No. 348 West Xianjia Lake Road, 410205, Changsha, Hunan Province, China

    Xin Deng, Songhua Long, Yufu Wang & Xinbo Chen

  2. Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, 410128, Changsha, Hunan Province, China

    Dongfeng He, Xiang Li, Jia Liu & Xinbo Chen

Authors
  1. Xin Deng
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  2. Songhua Long
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  3. Dongfeng He
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  4. Xiang Li
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  5. Yufu Wang
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  6. Jia Liu
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  7. Xinbo Chen
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Correspondence to Xinbo Chen.

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Deng, X., Long, S., He, D. et al. Development and characterization of polymorphic microsatellite markers in Linum usitatissimum . J Plant Res 123, 119–123 (2010). https://doi.org/10.1007/s10265-009-0271-3

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  • DOI: https://doi.org/10.1007/s10265-009-0271-3

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