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Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum

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Abstract

In this study, primer pairs of 15 microsatellite markers associated with sex determination of tilapia were selected and amplified in Wami tilapia, Oreochromis urolepis hornorum. While one marker, UNH168, on linkage group 3 (LG3) was associated (P < 0.001) with the phenotypic sex in the experimental population, nine genotypes were detected in both sexes. Only 99-bp allele was detected in the female samples, while 141, 149 and 157-bp alleles were present in both male and female samples. UNH168 was localized by fluorescence in situ hybridization (FISH) on the long arm of the largest tilapia chromosome pair (chromosome 1, equivalent to LG3). This sex-linked microsatellite marker could potentially be used for marker-assisted selection in tilapia breeding programmes to produce monosex male tilapia.

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Acknowledgements

This work was supported by grants from the National Key Technology R&D Programme (grant no. 2012BAD26B03), the National Natural Science Foundation of China (grant no. 31001108), the China Agriculture Research System (grant no. CARS-49) and Norway - China Government Scholarships (grant no. 229571/F11). We thank Dr X. Yu for providing BAC clones. Further, we are grateful to Dr X. Li for his assistance with microscopy techniques.

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Authors and Affiliations

  1. Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, People’s Republic of China

    HUAPING ZHU, ZHIGANG LIU, MAIXIN LU, FENGYING GAO, XIAOLI KE, DONGMEI MA, ZHANGHAN HUANG, JIANMENG CAO & MIAO WANG

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  1. HUAPING ZHU
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  2. ZHIGANG LIU
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  3. MAIXIN LU
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  4. FENGYING GAO
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  5. XIAOLI KE
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  6. DONGMEI MA
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  7. ZHANGHAN HUANG
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  8. JIANMENG CAO
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  9. MIAO WANG
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Correspondence to HUAPING ZHU or MAIXIN LU.

Additional information

[Zhu H., Liu Z., Lu M., Gao F., Ke X., Ma D., Huang Z., Cao J. and Wang M. 2016 Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum. J. Genet. 95, xx–xx]

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ZHU, H., LIU, Z., LU, M. et al. Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum . J Genet 95, 283–289 (2016). https://doi.org/10.1007/s12041-016-0653-y

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  • DOI: https://doi.org/10.1007/s12041-016-0653-y

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