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Isolation and Physical Mapping of Sex-Linked AFLP Markers in Nile Tilapia (Oreochromis niloticus L.)

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Abstract

Gynogenetically produced XX and YY Nile tilapia (Oreochromis niloticus) and diploid control groups were screened for amplified fragment length polymorphisms (AFLPs) to search for sex-linked or sex-specific markers. Family-level bulked segregant analysis (XX and YY gynogenetic family pools) and individual screening (XX and YY gynogenetics and XX and XY control individuals) identified 3 Y-linked (OniY425, OniY382, OniY227) and one X-linked (OniX420) AFLP markers. OniX420 and OniY425 were shown to be allelic. Single locus polymerase chain reaction assays were developed for these markers. Tight linkage was demonstrated between the AFLP markers and the sex locus within the source families. However, these markers failed to consistently identify sex in unrelated individuals, indicating recombination between the markers and the sex-determining loci. O. niloticus bacterial artificial chromosome clones, containing the AFLP markers, hybridized to the long arm of chromosome 1. This confirmed previous evidence, based on meiotic chromosome pairing and fluorescence in situ hybridization probes obtained through chromosome microdissection, that chromosome pair 1 is the sex chromosomes.

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Acknowledgments

S.C.H. was supported by a research grant from the Biotechnology and Biological Sciences Research Council of the United Kingdom (98/D11975) awarded to D.J.P., Professor N.R. Bromage (University of Stirling), and Dr. D.K. Griffin (Brunel University, U.K). C.B. was supported by a PhD award from the Royal Thai Government. We thank Dr. R. Griffiths for valuable discussion at the start of this research and Mr. K. Ranson for assistance in fish maintenance.

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Authors and Affiliations

  1. Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland, U.K.

    M. Tariq Ezaz, Simon C. Harvey, Chuta Boonphakdee, Alan J. Teale, Brendan J. McAndrew & David J. Penman

  2. Current address: Comparative Genomics Group, Research School of Biological Sciences, The Australian National University, Canberra, ACT 2601, Australia

    M. Tariq Ezaz

  3. Current address: School of Biological Sciences, University of Bristol, Bristol BS8 1UG, England, U.K.

    Simon C. Harvey

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  1. M. Tariq Ezaz
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Correspondence to David J. Penman.

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Ezaz, M.T., Harvey, S.C., Boonphakdee, C. et al. Isolation and Physical Mapping of Sex-Linked AFLP Markers in Nile Tilapia (Oreochromis niloticus L.). Mar Biotechnol 6, 435–445 (2004). https://doi.org/10.1007/s10126-004-3004-6

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