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Genetic and Physical Mapping of Sex-Linked AFLP Markers in Nile Tilapia (Oreochromis niloticus)

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Abstract

Identification of the sex-determining genes of the Nile tilapia (Oreochromis niloticus) has important implications for commercial aquaculture. We previously identified an XX/XY sex-determining locus in this species within a 10-cM interval between markers GM201 and UNH995 on linkage group one (LG1). In order to refine this region, we developed new AFLP markers using bulked segregant analysis of the mapping families. We identified three AFLP markers that showed a sex-specific pattern of segregation. All three mapped near, but just outside, the previously identified sex-determining region on LG1. Hybridization of BAC clones containing these markers to chromosome spreads confirmed that the XX/XY sex-determining locus is on one of the small chromosomes in O. niloticus.

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Acknowledgements

This project was supported by National Research Initiative competitive grant no. 2006-04830 from the USDA National Institute of Food and Agriculture Animal Genome Program and grant no. IS-3995-07 US-Israel Binational Agricultural Research and Development Fund.

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Authors and Affiliations

  1. Laboratory of Bioinformatics and Population Genetics, Department of Food and Animal Biotechnology, Seoul National University, Seoul, 151-742, Republic of Korea

    Bo-Young Lee

  2. Département Systématique et Evolution, Muséum national d’ Histoire naturelle, 57 rue Cuvier, 75231, Paris Cedex 05, France

    Jean-Pierre Coutanceau & Catherine Ozouf-Costaz

  3. CIRAD-Persyst, Aquaculture et Gestion des Resources Aquatiques, Montpellier, France

    Helena D’Cotta & Jean-Francois Baroiller

  4. Department of Biology, University of Maryland, College Park, MD, 20742, USA

    Bo-Young Lee & Thomas D. Kocher

Authors
  1. Bo-Young Lee
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  2. Jean-Pierre Coutanceau
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  3. Catherine Ozouf-Costaz
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  4. Helena D’Cotta
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  5. Jean-Francois Baroiller
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  6. Thomas D. Kocher
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Correspondence to Thomas D. Kocher.

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Lee, BY., Coutanceau, JP., Ozouf-Costaz, C. et al. Genetic and Physical Mapping of Sex-Linked AFLP Markers in Nile Tilapia (Oreochromis niloticus). Mar Biotechnol 13, 557–562 (2011). https://doi.org/10.1007/s10126-010-9326-7

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  • DOI: https://doi.org/10.1007/s10126-010-9326-7

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