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The effect of 24-epibrassinolide and clotrimazole on the adaptation of Cajanus cajan (L.) Millsp. to salinity

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Abstract

The objective of this study was to evaluate the effects of one of brassinosteroids (24-epibrassinolide) and clotrimazole, (an inhibitor of brassinosteroid synthesis) on plant growth parameters, parameters related to leaf gas exchange (photosynthetic and transpiration rates; stomatal conductance; water use efficiency), photosynthetic pigment content and osmolyte (sugars and proline) content in Cajanus cajan exposed to salinity. Salt stress—caused by NaCl treatment—affected values of all parameters analyzed. The effects were ameliorated by 24-epibrassinolide and intensified by clotrimazole. The hormone increased fresh mass of the plant, shoot dry mass, leaf area, water content of leaves and roots, photosynthetic pigments, sugar concentration, photosynthetic rate, and water use efficiency. The effects of hormone were less evident in the absence of salt. However, under this condition the application of clotrimazole affected the values of parameters studied, indicating the importance of brassinosteroid synthesis for the normal development of the plant.

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Acknowledgments

We wish to thank the Fundação de Amparo à Pesquisa do Estado de São Paulo for financial support of the project (08/50165-8). Ronaldo J. D. Dalio acknowledges the Conselho Nacional de Desenvolvimento Científico e Tecnológico for the grant received and we thank Dr. Elaine B. Wutke for seeds of C. cajan.

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Authors and Affiliations

  1. Department of Plant Biology, Institute of Biology, State University of Campinas-UNICAMP, CP 6109, Campinas, SP, 13083-970, Brazil

    Ronaldo José Durigan Dalio, Ladaslav Sodek & Claudia Regina Baptista Haddad

  2. Departament of Statistics, Institute of Mathematics, Statistics and Scientific Computation, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil

    Hildete Prisco Pinheiro

Authors
  1. Ronaldo José Durigan Dalio
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  2. Hildete Prisco Pinheiro
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  3. Ladaslav Sodek
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  4. Claudia Regina Baptista Haddad
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Correspondence to Ronaldo José Durigan Dalio.

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Communicated by W. Filek.

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Dalio, R.J.D., Pinheiro, H.P., Sodek, L. et al. The effect of 24-epibrassinolide and clotrimazole on the adaptation of Cajanus cajan (L.) Millsp. to salinity. Acta Physiol Plant 33, 1887–1896 (2011). https://doi.org/10.1007/s11738-011-0732-x

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  • DOI: https://doi.org/10.1007/s11738-011-0732-x

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