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Does exogenous application of 24-epibrassinolide ameliorate salt induced growth inhibition in wheat (Triticum aestivum L.)?

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Abstract

A greenhouse experiment was conducted to assess whether exogenously applied 24-epibrassionlide (24-epiBL) could alleviate the adverse effects of salt on wheat. Two hexaploid wheat (Triticum aestivum L.) cultivars, S-24 (salt tolerant) and MH-97 (moderately salt sensitive), were grown under control (0 mM NaCl in full strength Hoagland’s nutrient solution) or saline conditions (150 mM of NaCl in full strength Hoagland’s nutrient solution). After 41 days growth of wheat plants under saline conditions, 24-epiBL was applied as a foliar spray. Four levels of BR were used as 0 (water spray), 0.0125, 0.025, and 0.0375 mg l−1. Application of 24-epiBL increased plant biomass and leaf area per plant of both cultivars under non-saline conditions. However, under saline conditions, improvement in growth due to exogenous 24-epiBL was observed only in S-24. Photosynthetic rate was reduced due to salt stress in both cultivars, but this inhibitory effect was ameliorated significantly by the exogenous application of 24-epiBL. Exogenously applied 24-epiBL also enhanced the photosystem-II efficiency in both cultivars measured as F v /F m ratio. Although the activities of antioxidant enzymes, Superoxide dismutase (SOD), Peroxidase (POD) and Catalase (CAT) were increased due to salt stress in both wheat cultivars, exogenously applied 24-epiBL had a varying effect on the antioxidant system. The activity of SOD remained unaffected in both cultivars due to 24-epiBL application, but that of POD and CAT was promoted in the salt stressed plants of cv. S-24 only. In conclusion, improvement in growth in both wheat cultivars due to foliar applied 24-epiBL was found to be associated with 24-epiBL-induced enhancement in photosynthetic capacity. The 24-epiBL-induced regulation of antioxidant enzymes or growth under saline conditions was cultivar specific.

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Authors and Affiliations

  1. Department of Botany, University of Agriculture, Faisalabad, Pakistan

    Muhammad Shahbaz & Muhammad Ashraf

  2. Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan

    Habib-ur-Rehman Athar

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  1. Muhammad Shahbaz
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Correspondence to Muhammad Shahbaz.

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Shahbaz, M., Ashraf, M. & Athar, HuR. Does exogenous application of 24-epibrassinolide ameliorate salt induced growth inhibition in wheat (Triticum aestivum L.)?. Plant Growth Regul 55, 51–64 (2008). https://doi.org/10.1007/s10725-008-9262-y

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