Abstract
The effect of 24-epibrassinolide (BR27) on cold resistance of rape seedlings was studied by ion leakage and photosynthetic pigment degradation measurements. Aqueous solutions of BR27 were injected into cotyledons or primary leaves of rape plants and these plants were incubated at 2 °C or 20 °C. Cold treatment (2 °C) without BR27 injection elevated the membrane permeability in both primary leaves and cotyledons significantly. Surprisingly, injection of leaves with water or 0.467 % aqueous ethanol solution led to a massive increase in membrane permeability after cold stress at 2 °C. The synergistic effect of leaf infiltration and cold on permeability was abolished by 0.05 and 1.00 µM of BR27 in primary leaves and by 1.00 µM of BR27 in cotyledons. On the other hand, BR27 solutions strongly elevated the membrane permeability at 20 °C, while water and ethanol solutions brought about only negligible increases. Water or ethanol infiltrations strongly reduced the leaf contents of chlorophyll (Chl) a, Chl b and carotenoids at 2 °C but less markedly at 20 °C. However, in seedlings exposed to 2 °C pigments content was significantly higher in BR27-treated leaves as compared to water/ethanol control. There were no differences between pigment contents of leaves injected with BR27 solutions or only water/ethanol at 20 °C. The above data strongly support the stress protecting effect of BR27.
Abbreviations
- BRs:
-
brassinosteroids
- BR27 :
-
24-epibrassinolide
- PAR:
-
photosynthetically active radiation
References
Anuradha, S., Rao, S.S.R.: Application of brassinosteroids to rice seeds (Oryza sativa L.) reduced the impact of salt stress on growth, prevented photosynthetic pigment loss and increased nitrate reductase activity.-Plant Growth Regul. 40: 29–32, 2003.
Barna, B., Ádám, A., Király, Z.: Juvenility and resistance of a superoxide-tolerant plant to diseases and other stresses.-Naturwissenschaften 80: 420–422, 1993.
Bilkisu, A.A., Xiao-Gang, G., Qing-Lei, G., Yong-Hua, Y.: Brassinolide amelioration of aluminum toxicity in mungbean seedling growth.-J. Plant Nutr. 26: 1725–1734, 2003.
Bishop, G.J., Yokota, T.: Plants steroid hormones, brassinosteroids: current highlights of molecular aspects on their synthesis/metabolism, transport, perception and response.-Plant Cell Physiol. 42: 114–120, 2001.
Dhaubhadel, S., Browning, K.S., Gallie, D.R., Krishna, P.: Brassinosteroid functions to protect the translational machinery and heat shock protein synthesis following thermal stress.-Plant J. 29: 681–691, 2002.
Dhaubhadel, S., Chaudhary, S., Dobinson, K.F., Krishna, P.: Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance of Brassica napus and tomato seedlings.-Plant mol. Biol. 40: 333–342, 1999.
Fariduddin, Q., Ahmad, A., Hayat, S.: Responses of Vigna radiata to foliar application of 28-homobrassinolide and kinetin.-Biol. Plant. 48: 465–468, 2004.
Grove, M.D., Spencer, G.F., Rohwedder, W.K., Mandawa, N., Worley, J.F., Warthen, J.D., Steffens, G.L., Flippen-Anderson, J.L., Cook, J.C.: Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen.-Nature 281: 216–217, 1979.
Hayat, S., Ahmad, A., Hussain, A., Mobin, M.: Growth of wheat seedlings raised from the grains treated with 28-homobrassinolide.-Acta Physiol. Plant. 23: 27–30, 2001.
Hayat, S., Ahmad, A., Mobin, M., Hussain, A., Fariduddin, Q.: Photosynthetic rate, growth, and yield of mustard plants sprayed with 28-homobrassinolide.-Photosynthetica 38: 469–471, 2000.
Hotta, Y., Tanaka, T., Luo, B.S., Takeuchi, Y., Konnai, M.: Improvement of cold resistance in rice seedlings by 5-aminolevulinic acid.-J. Pest. Sci. 23: 29–33, 1998.
Janeczko, A., Filek, W., Biesaga-Kościelniak, J., Marcińska, I., Janeczko, Z.: The influence of animal sex hormones on the induction of flowering in Arabidopsis thaliana: comparison with the effect of 24-epibrassinolide.-Plant Cell Tissue Organ Cult. 72: 147–151, 2003.
Janeczko, A., Kościelniak, J., Pilipowicz, M., Szarek-Łukaszewska, G., Skoczowski, A.: Protection of winter rape photosystem 2 by 24-epibrassinolide under cadmium stress.-Photosynthetica 43: 293–298, 2005.
Jatimliansky, J.R., Garcia, M.D., Molina, M.C.: Response to chilling of Zea mays, Tripsacum dactyloides and their hybrid.-Biol. Plant. 48: 561–567, 2004.
Kęesy, J., Trzaskalska, A., Galoch, E., Kopcewicz, J.: Inhibitory effect of brassinosteroids on the flowering of the short-day plant Pharbitis nil.-Biol. Plant. 47: 597–600, 2003.
Krishna, P.: Brassinosteroid-mediated stress responses.-J. Plant Growth Regul. 22: 289–297, 2003.
Lichtenthaler, H.K., Wellburn, A.R.: Determinations of total carotenoids and chlorophyll a and b leaf extracts in different solvents.-Biochem. Soc. Trans. 603: 591–592, 1983.
Mandava, N.B.: Plant growth-promoting brassinosteroids.-Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 23–52, 1988.
Mazorra, L.M., Núñez, M.: Brassinosteroid analogues differentially modify peroxidase activity, superoxide dismutase activity and protein content in tomato seedlings.-Cult. Tropic. 21: 29–33, 2000.
Mazorra, L.M., Núñez, M., Hechavarria, M., Coll, F., Sanchez-Blanco, M.J.: Influence of brassinosteroids on antioxidant enzymes activity in tomato under different temperatures.-Biol. Plant. 45: 593–596, 2002.
Ramraj, V.M., Vyas, B.N., Godrej, N.B., Mistry, K.B., Swami, B.N., Singh, N.: Effects of 28-homobrassinolide on yields of wheat, rice, groundnut, mustard, potato and cotton.-J. agr. Sci. 128: 405–413, 1997.
Sasse, J.M.: Physiological actions of brassinosteroids: an update.-J. Plant Growth Regul. 22: 276–288, 2003.
Vardhini, B.V., Rao, S.S.R.: Effect of brassinosteroids on growth, metabolite content and yield of Arachis hypogaea.-Phytochemistry 48: 927–930, 1998.
Wilen, R.W., Sacco, M., Gusta, L.V., Krishna, P.: Effects of 24-epibrassinolide on freezing and thermotolerance of bromegrass (Bromus inermis) cell cultures.-Physiol. Plant. 95: 195–202, 1995.
Yoshioka, H., Numata, N., Nakajima, K., Katou, S., Kawakita, K., Rowland, O., Jones, J.D.G., Doke, N.: Nicotiana bethamiana gp91phox homologs NbrbohA and NbrboB participate in H2O2 accumulation and resistance to Phytophthora infestans.-Plant Cell 15: 706–718, 2003.
Yu, J.Q., Huang, L.F., Hu, W.H., Zhou, Y.H., Mao, W.H., Ye, S.F., Nogues, S.: A role for brassinosteroids in the regulation of photosynthesis in Cucumis sativus.-J. exp. Bot. 55: 1135–1143, 2004.
Zullo, M.A.T., Adam, G.: Brassinosteroid phytohormones — structure, bioactivity and applications.-Braz. J. Plant Physiol. 14: 83–121, 2002.
Zullo, M.A.T., Kohout, L.: Semisystematic nomenclature of brassinosteroids.-Plant Growth Regul. 42: 15–28, 2004.
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Janeczko, A., Gullner, G., Skoczowski, A. et al. Effects of brassinosteroid infiltration prior to cold treatment on ion leakage and pigment contents in rape leaves. Biol Plant 51, 355–358 (2007). https://doi.org/10.1007/s10535-007-0072-2
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DOI: https://doi.org/10.1007/s10535-007-0072-2