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Investigation of the response to salinity and to oxidative stress of interspecific potato somatic hybrids grown in a greenhouse

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Abstract

Salinity is one of the major stresses threatening potato plants (Solanum tuberosum L.) by affecting their growth and yield. It leads to oxidative stress by the production of reactive oxygen species responsible for alteration of macromolecules. To improve the tolerance of potato to salt stress, we have used somatic hybridization to produce interspecific potato hybrids by protoplast fusion between the BF15 variety and the wild Solanum berthaultii species. These hybrids showed an improved tolerance to salt stress when cultivated in vitro. The present work aims to analyze the response of the hybrids to salt stress in greenhouse conditions. Thus, the development of plants and their antioxidant capacity in response to salt stress were followed. All hybrids showed better growth and stable chlorophyll content compared to those of the BF15 parent plant. Membrane lipid peroxidation, evaluated by measuring the malondialdehyde accumulation (MDA) in plant organs, showed low levels in the hybrids. Higher antioxidant enzyme activities were measured in the roots of the hybrids when compared to those of the BF15 parent. These hybrids also showed an improved control of Na+ accumulation and a stable K+/Na+ ratio. These results therefore confirm the better tolerance of these hybrids to salt stress when compared to their BF15 parent.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

GPX:

Glutathione peroxidase

GR:

Glutathione reductase

GSH:

Glutathione

MDA:

Malondialdehyde

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgments

This work was financed by the Tunisian Ministry of Higher Education and Scientific Research. Hybrid production was performed at the Laboratory of Experimental Plant Morphogenesis, University of Southern Paris, Building 360, Orsay, France under the supervision of Dr. Darasinh Sihachakr. The authors are grateful to Dr. Anne-Lise Haenni from Institute Jacques (France) for reading and improving the manuscript and to Moufida Bouaziz-Kanoun from the “Institut Supérieur d’Administration des Affaires de Sfax” (Tunisia) for editing the paper. They wish to extend their thanks to Mr. Jamil Jaoua, Founder and Former Head of the English Unit at the Sfax Faculty of Science for a final proofreading of this paper.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Sfax Faculty of Science, PB 1171, 3038, Sfax, Tunisia

    Rania Jbir-Koubaa & Noureddine Drira

  2. Sfax National Engineering School, PB 1173, 3038, Sfax, Tunisia

    Safa Charfeddine, Wiem Ellouz, Mohamed Najib Saidi & Radhia Gargouri-Bouzid

  3. Sfax Preparatory Institute for Engineering Studies, PB 1172, 3038, Sfax, Tunisia

    Oumèma Nouri-Ellouz

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  1. Rania Jbir-Koubaa
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  2. Safa Charfeddine
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  3. Wiem Ellouz
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  5. Noureddine Drira
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Correspondence to Rania Jbir-Koubaa.

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Jbir-Koubaa, R., Charfeddine, S., Ellouz, W. et al. Investigation of the response to salinity and to oxidative stress of interspecific potato somatic hybrids grown in a greenhouse. Plant Cell Tiss Organ Cult 120, 933–947 (2015). https://doi.org/10.1007/s11240-014-0648-4

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