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Modulation of gene expression in cold-induced sweetening resistant potato species Solanum berthaultii exposed to low temperature

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Abstract

Cold-induced sweetening (CIS) is a crucial factor influencing the processing quality of potato tubers. To better understand the molecular events of potato CIS and different CIS-sensitivity among various potato species, a suppression subtractive hybridization library and cDNA microarray gene filters were developed. A total of 188 genes were found to be differentially expressed (DE) in Solanum berthaultii (ber) upon cold stimulation. These functional genes were mostly related to cell rescue, defense and virulence, metabolism, energy and protein fate, included in various processes of plant defense against abiotic stresses. Four expression patterns of these DE genes were profiled by qRT-PCR using the cold-stored tubers of both CIS-resistant (ber) and CIS-sensitive (E-potato 3, a variety of S. tuberosum) potatoes. The expression pattern and abundance of many DE genes encoding proteins involved in metabolism were different in these two potato tubers, especially genes associated with amylolysis, sucrose decomposition and glycolysis pathways, indicating distinct regulatory mechanisms between ber and E3 in response to cold stress, which may be crucial for potato CIS. Further investigation of these cold-regulated genes will deepen our understanding of the regulatory mechanisms of potato CIS and direct approaches for the genetic improvement of potato processing quality.

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Acknowledgments

We thank Dr. Xun Liu for designing primers for qRT-PCR analysis, Hui Fang and Yongbin Ou for technical assistance in qRT-PCR and sugar measurements. We also thank Mr. Andy Z. Jiang and International Science Editing for language editing. This research was supported by grants from the National Science Foundation of China (30571181 and 31171602) and Chenguang Project of Wuhan City (201050231068).

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Authors and Affiliations

  1. National Centre for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China

    Xia Chen, Botao Song, Jun Liu, Jianwen Yang, Tianjiu He, Yuan Lin, Huiling Zhang & Conghua Xie

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  1. Xia Chen
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  2. Botao Song
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  3. Jun Liu
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  8. Conghua Xie
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Correspondence to Conghua Xie.

Additional information

Communicated by C. Gebhardt.

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Appendix S1: Lists of DE genes in four groups classified according to expression profiles.

Appendix S2: Genes differentially expressed in ber under cold conditions.

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Supplementary material 2 (XLS 66 kb)

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Chen, X., Song, B., Liu, J. et al. Modulation of gene expression in cold-induced sweetening resistant potato species Solanum berthaultii exposed to low temperature. Mol Genet Genomics 287, 411–421 (2012). https://doi.org/10.1007/s00438-012-0688-6

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  • DOI: https://doi.org/10.1007/s00438-012-0688-6

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