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Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plastome and improved tolerance to salinity

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Abstract

In this study three somatic hybrid lines originating from protoplast fusion between Solanum tuberosum cv. BF15 and Solanum berthaultii were subjected to a detailed molecular analysis using the I-SSR-PCR technique based on 5′-anchored microsatellite primers. The data obtained revealed a polymorphism between the different lines, suggesting that they correspond to symmetric hybrids. The analysis of chloroplast genome of these hybrids showed that they are resulting from a recombination between parental plastomes. When transferred to a greenhouse, these hybrid lines displayed an improved vigour compared to the cultivated potato BF15 parent. Indeed, an important growth rate and high tuber yield and weight were obtained for these hybrids compared to the parent. Some of these hybrids showed also an improved ion homeostasis control and they seem to display a better tolerance to salt stress compared to the potato BF15 parent.

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Acknowledgment

This work was supported by the Tunisian Ministry of Higher Education, Scientific Research and Technology.

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Authors and Affiliations

  1. Laboratoire des Biotechnologies Végétales Appliquées à l’Amélioration des Cultures, Département de Génie Biologique, Ecole Nationale d’Ingénieurs de Sfax, PO Box W, 3038, Sfax, Tunisia

    Amira Bidani & Radhia Gargouri-Bouzid

  2. Département de Biologie et Géologie, Institut Préparatoire aux Etudes d’Ingénieurs de Sfax, PO Box 805, 3018, Sfax, Tunisia

    Oumèma Nouri-Ellouz

  3. Département de Biologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis, Tunisia

    Lilia Lakhoua

  4. Laboratoire d’Ecologie, Systématique et Evolution, Université Paris Sud, UPS (UMR 8079) Bât. 360, 91405, Orsay Cedex, France

    Darasinh Sihachakr

  5. Unité Mixte de Recherche 619 Physiologie et Biotechnologies Végétales, Institut National de la Recherche Agronomique, Université Bordeaux, 33883, Villenave d’Ornon, France

    Catherine Cheniclet

  6. Faculté des Sciences de Sfax, Université de Sfax, PO Box 802, Sfax, Tunisia

    Ali Mahjoub & Noureddine Drira

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  1. Amira Bidani
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  2. Oumèma Nouri-Ellouz
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  3. Lilia Lakhoua
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  4. Darasinh Sihachakr
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  5. Catherine Cheniclet
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  6. Ali Mahjoub
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  7. Noureddine Drira
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  8. Radhia Gargouri-Bouzid
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Correspondence to Radhia Gargouri-Bouzid.

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Bidani, A., Nouri-Ellouz, O., Lakhoua, L. et al. Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plastome and improved tolerance to salinity. Plant Cell Tiss Organ Cult 91, 179–189 (2007). https://doi.org/10.1007/s11240-007-9284-6

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  • DOI: https://doi.org/10.1007/s11240-007-9284-6

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