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Construction of high-density linkage map and identification of QTLs for resistance to sorghum downy mildew in maize (Zea mays L.)

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Abstract

Sorghum downy mildew (SDM), caused by obligate biotrophic fungi Peronosclerospora sorghi, is an economically important disease of maize. The genetics of resistance was reported to be polygenic thereby necessitating identification of QTLs for resistance to SDM to initiate effective marker-assisted selection programs. During post-rainy and winter season of 2012, 645 F2:3 progeny families from the cross CML153 (susceptible) × CML226 (resistant) were screened for their reaction to SDM. Characterization of QTLs affecting resistance to SDM was undertaken using the genetic linkage map with 319 polymorphic SSR and SNP marker loci and the phenotypic data of F2:3 families. Three QTLs conferring resistance to SDM were consistently identified on chromosomes 2, 3 and 6 in both seasons. The resistant parent CML226 contributed all the QTL alleles conferring resistance to SDM. The major QTL located on chromosome 2 explained 38.68% of total phenotypic variation in the combined analysis with a LOD score of 9.12. All the three QTL showed partially dominant gene effects in combined analysis. The detection of more than one QTL supports the hypothesis that quantitative genes control resistance to P. sorghi. The generation was advanced to F6 using markers linked to major QTLs on chromosomes 2 and 3 to derive 33 SDM resistant maize inbred lines.

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Acknowledgements

The authors are thankful to the Department of Biotechnology, Ministry of Science and Technology, Government of India and Pioneer Hi-Bred Pvt. Ltd. for their financial help. The first author is highly indebted to Pioneer Hi-Bred Pvt. Ltd. for providing him with Pioneer Hi-Bred Research International Scholarship.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, College of Agriculture, V.C. Farm, Mandya, Karnataka, 571 405, India

    Nagabhushan & H. C. Lohithaswa

  2. Pioneer Hi-Bred Pvt. Ltd, Kallinayakanahalli 561 213, Chikkaballapur, Gauribidanur, Karnataka, India

    Anand S. Pandravada

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  1. Nagabhushan
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  2. H. C. Lohithaswa
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  3. Anand S. Pandravada
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Correspondence to H. C. Lohithaswa.

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The experiments were performed following standard protocols.

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A high-density genetic linkage map of maize was constructed and identified QTLs for resistance to sorghum downy mildew. Marker-assisted selection was practised using markers linked to major QTLs on chromosomes 2 and 3, to derive 33 resistant inbreds.

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Nagabhushan, Lohithaswa, H.C. & Pandravada, A.S. Construction of high-density linkage map and identification of QTLs for resistance to sorghum downy mildew in maize (Zea mays L.). Mol Breeding 37, 2 (2017). https://doi.org/10.1007/s11032-016-0601-9

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