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Identification and validation of QTLs conferring resistance to sorghum downy mildew (Peronosclerospora sorghi) and Rajasthan downy mildew (P. heteropogoni) in maize

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Abstract

We have mapped the quantitative trait loci (QTLs) conferring resistance to sorghum downy mildew (Peronosclerospora sorghi; SDM) and Rajasthan downy mildew (P. heteropogoni; RDM), two species of DM prevalent throughout India. QTL mapping was carried out on a backcross population of 151 individuals derived from a cross between CM139 (susceptible parent) and NAI116 (highly resistant to both SDM and RDM). Heritability estimates were 0.74 for SDM and 0.67 for RDM. Composite interval mapping combined with a linkage map constructed with 80 simple sequence repeat (SSR) markers resulted in the identification of three QTLs (one each on chromosomes 2, 3 and 6) for SDM resistance and two QTLs (one each on chromosomes 3 and 6) for RDM resistance, all of which were contributed by NAI116. The significance of the major QTL on chromosome 6 (bin 6.05) that confers resistance to diverse DMs in tropical Asia, including SDM and RDM in India, was also verified. The results confirmed that some common QTLs contribute to both SDM and RDM resistance, while additional loci might specifically govern resistance to SDM. The QTL information generated in this study provide information that will aid in undertaking an integrated breeding strategy for the transfer of resistance to SDM and RDM in maize lines using marker-assisted selection.

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Acknowledgements

The study was carried out under the Asian Maize Biotechnology Network (AMBIONET) program in India, with financial support from the Asian Development Bank. We thank Mr. Govindraju and Dr. R. Kumar for their help with the field experiments. The first author (SKN) was also supported by a UGC Junior Research Fellowship and Senior Research Fellowship.

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Authors and Affiliations

  1. Maize Genetics Unit, Indian Agricultural Research Institute, New Delhi, 110012, India

    S. K. Nair, B. M. Prasanna & A. Garg

  2. Department of Plant Pathology, Maharana Pratap University of Agriculture and Technology, Udaipur, 313001, India

    R. S. Rathore

  3. Regional Research Station, University of Agricultural Sciences, Mandya, 571405, India

    T. A. S. Setty

  4. Directorate of Maize Research, Pusa Campus, New Delhi, 110012, India

    N. N. Singh

Authors
  1. S. K. Nair
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  2. B. M. Prasanna
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  3. A. Garg
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  4. R. S. Rathore
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  5. T. A. S. Setty
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  6. N. N. Singh
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Corresponding author

Correspondence to B. M. Prasanna.

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Communicated by D.A. Hoisington

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Nair, S.K., Prasanna, B.M., Garg, A. et al. Identification and validation of QTLs conferring resistance to sorghum downy mildew (Peronosclerospora sorghi) and Rajasthan downy mildew (P. heteropogoni) in maize. Theor Appl Genet 110, 1384–1392 (2005). https://doi.org/10.1007/s00122-005-1936-5

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  • DOI: https://doi.org/10.1007/s00122-005-1936-5

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