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Validation of quantitative trait loci for aluminum tolerance in Chinese wheat landrace FSW

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Abstract

Aluminum (Al) toxicity is one of the major constraints for wheat production in acidic soils worldwide and use of Al-tolerant cultivars is one of the most effective approaches to reduce Al damage in the acidic soils. A Chinese landrace, FSW, shows a high level of tolerance to Al toxicity and a mapping population of recombinant inbred lines (RILs) was developed from a cross between FSW and Al-sensitive US spring wheat cultivar Wheaton to validate the quantitative trait loci (QTL) previously identified in FSW. The mapping population was evaluated for net root growth (NRG) during Al stress in a nutrient solution culture and hematoxylin staining score (HSS) of root tips after Al stress. After 132 simple sequence repeat (SSR) markers from three chromosomes that were previously reported to have the QTLs were analyzed in the population, two QTLs for Al tolerance from FSW were confirmed. The major QTL on chromosome 4DL co-segregated with the Al-activated malate transporter gene (ALMT1), however, sequence analysis of the promoter region (Ups4) of ALMT1 gene indicated that FSW contained a marker allele that is different from the one that was reported to condition Al tolerance in the Brazilian source. Another QTL on chromosome 3BL showed a minor effect on Al tolerance in the population. The two QTLs accounted for about 74.9 % of the phenotypic variation for HSS and 72.1 % for NRG and demonstrated an epistatic effect for both HSS and NRG. SSR markers closely linked to the QTLs have potential to be used for marker-assisted selection (MAS) to improve Al tolerance in wheat breeding programs.

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Acknowledgments

This project was partially supported by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture and the scholarship to the first author from State Administration of Foreign Experts Affairs, China (no. CG2008320006). The authors would like to thank Dr. Paul St. Amand, USDA Central Small Grain Genotyping Center, and Dr. Chengsong Zhu, Department of Agronomy, Kansas State University, Manhattan KS, for technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. This is contribution no. 12-413-J from the Kansas Agricultural Experiment Station, Manhattan, KS.

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Authors and Affiliations

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People’s Republic of China

    Jian Dai

  2. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Jian Dai, Guihua Bai & Dadong Zhang

  3. USDA/ARS Hard Winter Wheat Genetics Research Unit and Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Guihua Bai

  4. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Jian Dai & Delin Hong

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  1. Jian Dai
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  2. Guihua Bai
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  3. Dadong Zhang
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  4. Delin Hong
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Correspondence to Guihua Bai.

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Dai, J., Bai, G., Zhang, D. et al. Validation of quantitative trait loci for aluminum tolerance in Chinese wheat landrace FSW. Euphytica 192, 171–179 (2013). https://doi.org/10.1007/s10681-012-0807-9

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  • DOI: https://doi.org/10.1007/s10681-012-0807-9

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