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Transcriptional analysis between two wheat near-isogenic lines contrasting in aluminum tolerance under aluminum stress

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Abstract

To understand the mechanisms of aluminum (Al) tolerance in wheat (Triticum aestivum L.), suppression subtractive hybridization (SSH) libraries were constructed from Al-stressed roots of two near-isogenic lines (NILs). A total of 1,065 putative genes from the SSH libraries was printed in a cDNA array. Relative expression levels of those genes were compared between two NILs at seven time points of Al stress from 15 min to 7 days. Fifty-seven genes were differentially expressed for at least one time point of Al treatment. Among them, 28 genes including genes for aluminum-activated malate transporter-1, ent-kaurenoic acid oxidase-1, β-glucosidase, lectin, histidine kinase, and phospoenolpyruvate carboxylase showed more abundant transcripts in Chisholm-T and therefore may facilitate Al tolerance. In addition, a set of genes related to senescence and starvation of nitrogen, iron, and sulfur, such as copper chaperone homolog, nitrogen regulatory gene-2, yellow stripe-1, and methylthioribose kinase, was highly expressed in Chisholm-S under Al stress. The results suggest that Al tolerance may be co-regulated by multiple genes with diverse functions, and those genes abundantly expressed in Chisholm-T may play important roles in enhancing Al tolerance. The down-regulated genes in Chisholm-S may repress root growth and restrict uptake of essential nutrient elements, and lead to root senescence.

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Acknowledgments

We gratefully acknowledge Drs. Patricia Ayoubi and Hua Wen for the technical assistance in array printing and data analysis. This paper reports the results of research only. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

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Authors and Affiliations

  1. College of Life Science, Guangzhou University, Guangzhou, 510006, China

    Peiguo Guo & Ronghua Li

  2. USDA-ARS—Plant Science and Entomology Research Unit, 4008 Throckmorton Hall, Kansas State University, Manhattan, KS, 66506, USA

    Guihua Bai

  3. Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

    Peiguo Guo, Brett Carver & Amy Bernardo

  4. International Center for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 5466, Aleppo, Syria

    Michael Baum

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  1. Peiguo Guo
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  2. Guihua Bai
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Correspondence to Guihua Bai.

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Communicated by J.-K. Zhu.

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Guo, P., Bai, G., Carver, B. et al. Transcriptional analysis between two wheat near-isogenic lines contrasting in aluminum tolerance under aluminum stress. Mol Genet Genomics 277, 1–12 (2007). https://doi.org/10.1007/s00438-006-0169-x

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