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Quantitative trait loci for aluminum resistance in Chinese wheat landrace FSW

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Abstract

Aluminum (Al) toxicity is a major constraint for wheat production in acid soils worldwide. Chinese landrace FSW demonstrates a high level of Al resistance. A population of recombinant inbred lines (RILs) was developed from a cross between FSW and an Al-sensitive Chinese line, ND35, using single seed descent, to map quantitative trait loci (QTLs) for Al resistance. Wheat reaction to Al stress was measured by net root growth (NRG) in a nutrient solution culture containing Al3+ and hematoxylin staining score (HSS) of root after Al stress. After 1,437 simple sequence repeats (SSRs) were screened using bulk segregant analysis, three QTLs were identified to control Al resistance in FSW. One major QTL (Qalt.pser-4DL) was mapped on chromosome 4DL that co-segregated with Xups4, a marker for the promoter of the Al-activated malate transporter (ALMT1) gene. The other two QTLs (Qalt.pser-3BL, Qalt.pser-2A) were located on chromosomes 3BL and 2A, respectively. Together, the three QTLs accounted for up to 81.9% of the phenotypic variation for HSS and 78.3% of the variation for NRG. The physical positions of flanking markers for Qalt.pser-4DL and Qalt.pser-3BL were determined by analyzing these markers in corresponding nulli-tetrasomic, ditelosomic, and 3BL deletion lines of Chinese Spring. Qalt.pser-3BL is a novel QTL with a major effect on Al resistance discovered in this study. The two major QTLs on 4DL and 3BL demonstrated an additive effect. The SSR markers closely linked to the QTLs have potential to be used for marker-assisted selection (MAS) to improve Al resistance of wheat cultivars in breeding programs.

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Acknowledgments

This project is partly funded by the National Research Initiative of USDA’s Cooperative State Research, Education and Extension Service, CAP grant number 2006-55606-16629. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. This is contribution No. 08-172-J from the Kansas Agricultural Experiment Station, Manhattan, Kansas, USA.

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Authors and Affiliations

  1. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Shibin Cai, Gui-Hua Bai & Dadong Zhang

  2. Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Shibin Cai & Dadong Zhang

  3. USDA-ARS Plant Science and Entomology Research Unit, Manhattan, KS, 66506, USA

    Gui-Hua Bai

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  1. Shibin Cai
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  2. Gui-Hua Bai
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  3. Dadong Zhang
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Correspondence to Gui-Hua Bai.

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Communicated by J.-L. Jannink.

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Cai, S., Bai, GH. & Zhang, D. Quantitative trait loci for aluminum resistance in Chinese wheat landrace FSW. Theor Appl Genet 117, 49–56 (2008). https://doi.org/10.1007/s00122-008-0751-1

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  • DOI: https://doi.org/10.1007/s00122-008-0751-1

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