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OsMLO12, encoding seven transmembrane proteins, is involved with pollen hydration in rice

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Abstract

Key message

MLO mediates pollen hydration.

Abstract

Hydration is the first step in pollen germination. However, the process is not well understood. OsMLO12 is highly expressed in mature pollen grains; plants containing alleles caused by transfer DNA insertions do not produce homozygous progeny. Reciprocal crosses between wild-type and OsMLO12/osmlo12 plants showed that the mutant alleles were not transmitted through the male gametophyte. Microscopic observations revealed that, although mutant grains became mature pollen with three nuclei, they did not germinate in vitro or in vivo due to a failure in hydration. The OsMLO12 protein has seven transmembrane motifs, with an N-terminal extracellular region and a C-terminal cytosolic region. We demonstrated that the C-terminal region mediates a calcium-dependent interaction with calmodulin. Our findings suggest that pollen hydration is regulated by MLO12, possibly through an interaction with calmodulin in the cytosol.

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Acknowledgments

We thank Kyungsook An and Sunghae Hong for managing the transgenic lines. This work was supported in part by grants from the Next-Generation BioGreen 21 Program (No. PJ008215), Rural Development Administration, Republic of Korea; the Basic Research Promotion Fund, Republic of Korea (NRF-2007-0093862); Kyung Hee University (20130214) to G.A.; and the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (2013R1A6A3A01026391) to J. Y.

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  1. Department of Plant Molecular Systems Biotechnology, Crop Biotech Institute, Kyung Hee University, 1732 Deogyeong-daero, Giheung, Yongin, Gyeonggi, Korea

    Jakyung Yi, Suyoung An & Gynheung An

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  1. Jakyung Yi
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  2. Suyoung An
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  3. Gynheung An
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Correspondence to Gynheung An.

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Communicated by Venkatesan Sundaresan.

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Yi, J., An, S. & An, G. OsMLO12, encoding seven transmembrane proteins, is involved with pollen hydration in rice. Plant Reprod 27, 169–180 (2014). https://doi.org/10.1007/s00497-014-0249-8

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