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Cloning Vectors for Rice

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Abstract

We developed various binary vectors that can be used for expressing a foreign gene in rice. Vectors pGA3426, pGA3436, and pGA3626 are intended for overexpression of a gene using the maize Ubiquitin promoter, whereas pGA3780 is for rather mild expression of a gene using the rice Actin1 promoter. Vector pGA3777 is for expressing two genes simultaneously. We also developed binary vectors for expressing a fusion protein with a tag. Four vectors (pGA3427, pGA3428, pGA3429, and pGA3438) are for protein tags with sGFP, HA, His, and Myc, respectively. Vector pGA3383 is for analyzing promoter activity using the GUS reporter. In this vector, multiple cloning sites in front of GUS can be utilized for accepting a promoter fragment. We also generated transient expression vectors for studying the subcellular localization of a protein. Vectors pGA3452, pGA3651, and pGA3652 are for GFP fusion; pGA3574 for RFP fusion; pGA3697 for Myc tag; and pGA3698 for HA tag. In addition, we generated pGA3506, pGA3516, pGA3592, and pGA3593 for facilitating the subcloning of full-length cDNA clones into our binary vectors.

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Acknowledgments

We thank Priscilla Licht for critical reading of the manuscript. This work was supported, in part, by grants from the Crop Functional Genomic Center, the 21st Century Frontier Program (Grant CG1111); from the Biogreen 21 Program (034-001-007-03-00), Rural Development Administration; from the Korea Science and Engineering Foundation (KOSEF) through the National Research Laboratory Program funded by the Ministry of Science and Technology (M10600000270-06J0000-27010); and from the Basic Research Promotion Fund by a Korea Research Foundation Grant (KRF-2007-341-C00028).

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Authors and Affiliations

  1. National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, Republic of Korea

    Sung-Ryul Kim, Dong-Yeon Lee, Jung-Il Yang, Sunok Moon & Gynheung An

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  1. Sung-Ryul Kim
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  2. Dong-Yeon Lee
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  5. Gynheung An
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Correspondence to Gynheung An.

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Kim, SR., Lee, DY., Yang, JI. et al. Cloning Vectors for Rice. J. Plant Biol. 52, 73–78 (2009). https://doi.org/10.1007/s12374-008-9008-4

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  • DOI: https://doi.org/10.1007/s12374-008-9008-4

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