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Therapeutic efficacy of poly (lactic-co-glycolic acid) nanoparticles encapsulated ivermectin (nano-ivermectin) against brugian filariasis in experimental rodent model

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Abstract

The present study reports on the antifilarial activity of poly (lactic-co-glycolic acid) nanoparticles encapsulated ivermectin (nano-IVM) against human lymphatic filariid Brugia malayi in rodent host Mastomys coucha. Nano-IVM was prepared and optimized by nanoprecipitation method. The selected nano-IVM (F5) showed a uniform spherical shape with 96 nm diameter and 74.12 % entrapment efficiency, and when used at a suboptimal dose of 100 μg/kg body weight, completely eliminated filarial parasites from systemic circulation on 60 days post-infection in animals inflicted with B. malayi. In contrast, the coadministration of nano-IVM (F5) along with standard filaricide diethylcarbamazine (DEC) was found to be competent enough to suppress microfilarial stage of parasites and successfully eliminated microfilaria at 45 days posttreatment. However, the free form of both the drugs alone or in combination was unable to impart such suppression and followed by recurrence of the infection. Interestingly, nano-IVM (F5) was also found to be effective against adult stage parasites causing 36.67 % worm mortality and 75.89 % in combination with DEC; however, female sterilization remain almost similar. Thus, the combination of entrapped IVM with DEC exhibited enhanced microfilaricidal and marginally better macrofilaricidal efficacy than any of the single formulation or drugs combination.

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Acknowledgments

The authors are thankful to the Indian Council of Medical Research (ICMR), New Delhi, India, for providing financial support in the form of Senior research fellowships to MA.

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Authors and Affiliations

  1. Nanomedicine Lab, Hamdard Nanobiotechnology Center for Advance Research, Faculty of Engineering & Interdisciplinary Sciences, Hamdard University (Jamia Hamdard), New Delhi, 110062, India

    Mohammad Ali

  2. Dabur Research and Development Centre, Dabur India limited, Ghaziabad, 201010, India

    Mohammad Afzal

  3. Division of Parasitology, Central Drug Research Institute (CDRI, CSIR), Lucknow, 226001, India

    Meenakshi Verma & Shailja Misra Bhattacharya

  4. Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University (Jamia Hamdard), New Delhi, 110062, India

    F. J. Ahmad

  5. Department of Chemistry, Faculty of Science, Hamdard University (Jamia Hamdard), New Delhi, 110062, India

    Mohammad Samim

  6. Department of Biotechnology, Faculty of Science, Hamdard University (Jamia Hamdard), New Delhi, 110062, India

    M. Z. Abidin

  7. Department of Pathology, All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi, 110029, India

    A. K. Dinda

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  1. Mohammad Ali
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Ali, M., Afzal, M., Verma, M. et al. Therapeutic efficacy of poly (lactic-co-glycolic acid) nanoparticles encapsulated ivermectin (nano-ivermectin) against brugian filariasis in experimental rodent model. Parasitol Res 113, 681–691 (2014). https://doi.org/10.1007/s00436-013-3696-5

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